Fat and oil derivatives - Fatty Acid Methyl Esters (FAME) - Determination of phosphorus content by inductively coupled plasma (ICP) emission spectrometry

This European Standard specifies an inductively coupled plasma (ICP) emission spectrometry method for the determination of phosphorus content between 4 mg/kg and 20 mg/kg in Fatty Acid Methyl Esters, hereinafter referred as FAME.
This method aims to evaluate the FAME quality, in terms of transesterification by-products content such as phosphorus, whose concentration may affect the fuel behaviour.
WARNING - The use of this method may involve hazardous equipment, materials and operations. This method does not purport to address to all of the safety problems associated with its use, but it is the responsibility of the user to search and establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

Erzeugnisse aus pflanzlichen und tierischen Fetten und Ölen - Fettsäure-Methylester (FAME) - Bestimmung des Phosphorgehaltes durch Emissionsspektrometrie mit induktiv gekoppeltem Plasma (ICP)

Diese Europäische Norm legt ein Verfahren zur Bestimmung des Gehaltes von Phosphor mit Hilfe der Emissionsspektrometrie mit induktiv gekoppeltem Plasma (ICP) im Bereich von 4 mg/kg bis 20 mg/kg in Fettsäure-Methylestern, nachfolgend als FAME bezeichnet, fest.
Die Anwendung dieses Verfahrens dient dazu, die Qualität des FAME zu beurteilen, bezüglich der aus dem Veresterungsprozess entstehenden Nebenprodukte wie Phosphor, dessen Konzentration die Kraftstoffqualität beeinflussen kann.
WARNUNG - Die Anwendung dieses Verfahrens kann den Einsatz gefährlicher Stoffe, Arbeitsgänge und Geräte mit sich bringen. Diese Norm gibt nicht vor, alle mit ihrer Anwendung verbundenen Sicherheitsprobleme anzusprechen. Der Anwender dieser Norm ist dafür verantwortlich, vorher angemessene Maßnahmen zu ergreifen und die Anwendbarkeit einschränkender Vorschriften zu ermitteln.

Produits dérivés des corps gras - Esters méthyliques d'acides gras (EMAG) - Détermination de la teneur en phosphore par spectrométrie d'émission de plasma induit par haute fréquence (méthode ICP)

La présente Norme européenne spécifie une méthode de dosage du phosphore dans les esters méthyliques d'acides gras, ci-après appelés EMAG, par spectrométrie d'émission de plasma induit par haute fréquence à des teneurs comprises entre 4 mg/kg et 20 mg/kg.
L'objet de cette méthode est d'évaluer la qualité des EMAG en termes de teneur en sous-produits de transestérification tels que le phosphore dont la concentration peut affecter le comportement du carburant.
AVERTISSEMENT   La présente méthode peut mettre en jeu des substances, des opérations et des équipements dangereux. Elle ne prétend pas prendre en compte tous les problèmes de sécurité associés à son utilisation. Il est de la responsabilité de l'utilisateur de la présente méthode de rechercher et de mettre en place les mesures appropriées de sécurité et de protection de la santé, et de déterminer l'applicabilité des dispositions faisant l'objet de la méthode avant utilisation.

Derivati maščob in olj - Metil estri maščobnih kislin (FAME) - Določevanje fosforja z emisijsko spektrometrijo z induktivno sklopljeno plazmo (ICP)

General Information

Status
Published
Publication Date
31-Oct-2003
ICS
67.200.10 - Animal and vegetable fats and oils
Technical Committee
KŽP - Agricultural food products
Current Stage
6060 - National Implementation/Publication (Adopted Project)
Start Date
01-Nov-2003
Due Date
01-Nov-2003
Completion Date
01-Nov-2003
Mandate
M/245 - Mandate to CEN for the elaboration and adoption of standards concerning minimum requirement specifications including test methods for Fatty Acid MethylEster (FAME) as fuel for diesel engines and for space heating
Ref Project
EN 14107:2003 - Fat and oil derivatives - Fatty Acid Methyl Esters (FAME) - Determination of phosphorus content by inductively coupled plasma (ICP) emission spectrometry

Overview

EN 14107:2003 (CEN) specifies an analytical method for the determination of phosphorus in Fatty Acid Methyl Esters (FAME) using inductively coupled plasma (ICP) emission spectrometry. The method covers phosphorus content in the range 4 mg/kg to 20 mg/kg and is intended to evaluate FAME quality by measuring transesterification by‑products (residual phosphorus from phospholipids) that can affect fuel behaviour. The standard includes sample preparation, calibration, measurement and performance criteria.

Keywords: EN 14107:2003, FAME analysis, phosphorus content, ICP emission spectrometry, biodiesel quality, transesterification by‑products.

Key topics and technical requirements

  • Scope and detection range: Phosphorus determination in FAME between 4–20 mg/kg.
  • Analytical principle: Samples and standards are nebulised into an argon ICP and phosphorus emission is measured (commonly at 213.6 nm or 178.3 nm).
  • Sample preparation: Weigh ~2.5 g sample, dilute at least 10× with xylene (polypropylene/disposable pipettes to avoid contamination). Use a stock oil addition to standards to match viscosity with samples.
  • Standards and calibration: Calibration solutions typically prepared at 0, 0.5, 1, 2, 4 mg/L (based on an intermediate 100 mg/L intermediate solution); linear regression to build calibration curve.
  • Calculation: Convert measured concentration (c, mg/L) to sample phosphorus (C, mg/kg) using C = c · V / m (V = solution volume in mL, m = sample mass in g).
  • Precision criteria: Repeatability-absolute difference not greater than 0.6 mg/kg (more than once out of 20). Reproducibility given by R = 0.192·C + 0.025. Interlaboratory data are provided in informative annex A.
  • Apparatus & reagents: ICP spectrometer with quartz torch and nebulizer, peristaltic pump (0.5–3 mL/min), xylene solvent, phosphorus organic standards and stabilizer. Special glassware washing (HCl rinse) to avoid phosphate contamination.
  • Safety: Method involves hazardous solvents (xylene) and high‑energy plasma equipment - users must follow lab safety and regulatory requirements.

Practical applications and users

  • Quality control laboratories in biodiesel and FAME production assessing residual phosphorus that can harm catalysts, engine components or fuel injection systems.
  • Fuel suppliers and blending facilities verifying FAME feedstocks and finished fuels.
  • Certification and regulatory bodies evaluating compliance of FAME batches.
  • R&D and analytical service providers developing process optimization and troubleshooting transesterification.

Related standards

  • EN ISO 661 - Preparation of test portion for animal and vegetable fats and oils (referenced normative).
  • EN ISO 5555 - Sampling for fats and oils (recommended sampling method).
  • EN ISO 4259 - Application of precision data (used for interlaboratory statistics).

Note: The method may require instrument vendor‑specific parameter adjustments; always follow manufacturer guidance for organic solvent operation and ensure appropriate safety measures.

Frequently Asked Questions

SIST EN 14107:2003 is a standard published by the Slovenian Institute for Standardization (SIST). Its full title is "Fat and oil derivatives - Fatty Acid Methyl Esters (FAME) - Determination of phosphorus content by inductively coupled plasma (ICP) emission spectrometry". This standard covers: This European Standard specifies an inductively coupled plasma (ICP) emission spectrometry method for the determination of phosphorus content between 4 mg/kg and 20 mg/kg in Fatty Acid Methyl Esters, hereinafter referred as FAME. This method aims to evaluate the FAME quality, in terms of transesterification by-products content such as phosphorus, whose concentration may affect the fuel behaviour. WARNING - The use of this method may involve hazardous equipment, materials and operations. This method does not purport to address to all of the safety problems associated with its use, but it is the responsibility of the user to search and establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

This European Standard specifies an inductively coupled plasma (ICP) emission spectrometry method for the determination of phosphorus content between 4 mg/kg and 20 mg/kg in Fatty Acid Methyl Esters, hereinafter referred as FAME. This method aims to evaluate the FAME quality, in terms of transesterification by-products content such as phosphorus, whose concentration may affect the fuel behaviour. WARNING - The use of this method may involve hazardous equipment, materials and operations. This method does not purport to address to all of the safety problems associated with its use, but it is the responsibility of the user to search and establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

SIST EN 14107:2003 is classified under the following ICS (International Classification for Standards) categories: 67.200.10 - Animal and vegetable fats and oils. The ICS classification helps identify the subject area and facilitates finding related standards.

SIST EN 14107:2003 is associated with the following European legislation: Standardization Mandates: M/245. When a standard is cited in the Official Journal of the European Union, products manufactured in conformity with it benefit from a presumption of conformity with the essential requirements of the corresponding EU directive or regulation.

SIST EN 14107:2003 is available in PDF format for immediate download after purchase. The document can be added to your cart and obtained through the secure checkout process. Digital delivery ensures instant access to the complete standard document.

Standards Content (Sample)


2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.Erzeugnisse aus pflanzlichen und tierischen Fetten und Ölen - Fettsäure-Methylester (FAME) - Bestimmung des Phosphorgehaltes durch Emissionsspektrometrie mit induktiv gekoppeltem Plasma (ICP)Produits dérivés des corps gras - Esters méthyliques d'acides gras (EMAG) - Détermination de la teneur en phosphore par spectrométrie d'émission de plasma induit par haute fréquence (méthode ICP)Fat and oil derivatives - Fatty Acid Methyl Esters (FAME) - Determination of phosphorus content by inductively coupled plasma (ICP) emission spectrometry67.200.10Animal and vegetable fats and oilsICS:Ta slovenski standard je istoveten z:EN 14107:2003SIST EN 14107:2003en01-november-2003SIST EN 14107:2003SLOVENSKI
STANDARD
EUROPEAN STANDARDNORME EUROPÉENNEEUROPÄISCHE NORMEN 14107April 2003ICS 67.200.10English versionFat and oil derivatives - Fatty Acid Methyl Esters (FAME) -Determination of phosphorus content by inductively coupledplasma (ICP) emission spectrometryProduits dérivés des corps gras - Esters méthyliquesd'acides gras (EMAG) - Détermination de la teneur enphosphore par spectrométrie d'émission de plasma induitpar haute fréquence (méthode ICP)Erzeugnisse aus pflanzlichen und tierischen Fetten undÖlen - Fettsäure-Methylester (FAME) - Bestimmung desPhosphorgehaltes durch Emissionsspektrometrie mitinduktiv gekoppeltem Plasma (ICP)This European Standard was approved by CEN on 2 January 2003.CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this EuropeanStandard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such nationalstandards may be obtained on application to the Management Centre or to any CEN member.This European Standard exists in three official versions (English, French, German). A version in any other language made by translationunder the responsibility of a CEN member into its own language and notified to the Management Centre has the same status as the officialversions.CEN members are the national standards bodies of Austria, Belgium, Czech Republic, Denmark, Finland, France, Germany, Greece,Hungary, Iceland, Ireland, Italy, Luxembourg, Malta, Netherlands, Norway, Portugal, Slovakia, Spain, Sweden, Switzerland and UnitedKingdom.EUROPEAN COMMITTEE FOR STANDARDIZATIONCOMITÉ EUROPÉEN DE NORMALISATIONEUROPÄISCHES KOMITEE FÜR NORMUNGManagement Centre: rue de Stassart, 36
B-1050 Brussels© 2003 CENAll rights of exploitation in any form and by any means reservedworldwide for CEN national Members.Ref. No. EN 14107:2003 ESIST EN 14107:2003
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