SIST EN ISO 10519:2026
(Main)Rapeseed - Determination of chlorophyll content - Spectrometric method (ISO 10519:2026)
- Abstract
This document specifies a spectrometric method for the determination of the chlorophyll content of rapeseed. It is not applicable to the determination of chlorophyll in oils.
- Status
- Published
- Public Enquiry End Date
- 30-Jul-2026
- Publication Date
- 02-Nov-2026
- Technical Committee
- KŽP - Agricultural food products
- Current Stage
- 6060 - National Implementation/Publication (Adopted Project)
- Start Date
- 02-Nov-2026
- Due Date
- 07-Jan-2027
- Completion Date
- 03-Nov-2026
SIST EN ISO 10519:2026 is the Slovenian adoption of International Organization for Standardization (ISO) 10519:2026, and it specifies a spectrometric method for determining the chlorophyll content of rapeseed. It is intended for laboratories and other users who need a reproducible way to measure chlorophyll in rapeseed samples; it does not apply to chlorophyll in oils.
What does SIST EN ISO 10519:2026 specify?
SIST EN ISO 10519:2026 covers one analytical method for rapeseed and is organized into 12 clauses plus two informative annexes. The method is built around sample preparation, solvent extraction, spectrometric measurement, calculation of chlorophyll content, and a reportable test result.
| Annex | What it covers |
|---|---|
| Annex A | Results of an interlaboratory test used to summarize precision data |
| Annex B | Preparation of the calibration curve used to determine the k factor |
The scope is narrow and practical: it is for rapeseed testing, not for oil analysis. That matters because sample type and matrix affect extraction and the way absorbance is interpreted.
What are the key requirements of SIST EN ISO 10519:2026?
SIST EN ISO 10519:2026 sets out a workflow that starts with representative sampling, then moves through grinding, extraction, measurement, and calculation. The main purpose is to make chlorophyll results comparable between laboratories.
Sampling and sample preparation
Clause 7 says sampling is outside the method itself, and refers users to ISO 542 for a recommended sampling method. Clause 8 requires preparation of the test sample in accordance with ISO 664, after impurities are removed.
For wetter seed, Clause 8 requires drying when moisture is above a mass fraction of 10 % before grinding. This reduces the risk of destroying chlorophyll pigments, which matters if the sample has been stored or transported in damp conditions.
Extraction and measurement
Clause 9 uses a 2 g test portion and 30 ml of extraction solvent. The extract is made with anhydrous ethanol combined with iso-octane, n-heptane, or petroleum ether, then shaken either for 1 hour in a tube with steel balls or for 20 minutes in a commercial ball mill.
The extract is allowed to settle, filtered, and measured in a spectrometer at 665 nm, 705 nm, and 625 nm. The extra wavelengths are used for baseline correction, so the result is based on corrected absorbance rather than a single raw reading.
Calculation and calibration
Clause 10 expresses chlorophyll content in milligrams per kilogram of product as received. The calculation uses corrected absorbance, optical path length, sample mass, solvent volume, and the constant k.
Clause 10 also states that k is 13 under the specified conditions, but if the spectrophotometer bandwidth varies, k may need to be determined for the chosen instrument and fixed setting. Annex B shows how to prepare the calibration curve for that purpose. In practice, this means users must know their instrument’s bandwidth and verify the calibration if needed.
Precision and reporting
Clause 11 gives precision guidance from an interlaboratory study: repeatability and reproducibility are stated as relative limits for comparing results. That helps laboratories judge whether two results are close enough to be considered consistent.
Clause 12 requires the test report to identify the sampling method if known, the method used, the result or results, the final quoted result if repeatability was checked, the test date, and any operating details or incidents that could have affected the outcome. This makes the report traceable and usable in quality records.
What terms does SIST EN ISO 10519:2026 define?
SIST EN ISO 10519:2026 defines a small set of technical terms for the method.
- Chlorophyll content - the mass fraction of substances in the sample that absorb near 665 nm, expressed as chlorophyll A in milligrams per kilogram.
- Corrected absorbance - the absorbance value used in the final calculation after baseline correction from the reference wavelengths.
- Test portion - the weighed portion of prepared sample used for extraction and measurement.
- Repeatability - the closeness of results from the same laboratory, operator, equipment, and short time interval.
- Reproducibility - the closeness of results from different laboratories using the same method on the same material.
- Bandwidth - the spectrometer characteristic that affects measurement resolution and the k factor used in the calculation.
Who uses SIST EN ISO 10519:2026?
SIST EN ISO 10519:2026 is used by analytical laboratories, oilseed testing labs, seed processors, quality managers, and buyers who need to compare rapeseed lots on a chlorophyll basis. It is also relevant to organizations that control raw material quality before further processing, because chlorophyll content is a measurable quality attribute in rapeseed.
The method is suited to routine laboratory work where the same procedure must be applied consistently across samples and across sites. Its precision section and test report requirements are especially useful for quality control, disputes, and specification checking.
What changed in SIST EN ISO 10519:2026 from the previous edition?
SIST EN ISO 10519:2026 cancels and replaces ISO 10519:2015. The document states that it is a minor revision and that it also incorporates ISO 10519:2015/Amd 1:2025.
The change listed in the foreword is the addition of Annex B, which gives the preparation of the calibration curve to determine the k factor.
Which standards are used with SIST EN ISO 10519:2026?
SIST EN ISO 10519:2026 refers to several companion standards that support sampling, sample reduction, and moisture determination.
- ISO 648 - specifies the pipettes used for accurate 30 ml dispensing.
- ISO 664 - gives the laboratory-sample-to-test-sample reduction step used in preparation.
- ISO 665 - is used when chlorophyll content needs to be expressed relative to dry product.
- ISO 542 - is cited as a recommended sampling method.
- ISO 5725:1986 - underpins the interlaboratory precision study summarized in Annex A.
What does the SIST EN ISO 10519:2026 document contain?
SIST EN ISO 10519:2026 contains the full analytical sequence: scope, references, term definition, principle, reagents, apparatus, sampling, sample preparation, procedure, calculation, precision, and reporting. It also includes an interlaboratory precision summary in Annex A and a calibration-curve procedure in Annex B.
The document provides a practical laboratory method with specified solvent composition, equipment requirements, wavelengths, extraction times, and reporting items. For users, the useful parts are the procedure, the calculation approach, the precision information, and the calibration guidance for instruments with variable bandwidth.
Relations
- Effective Date
- 01-Dec-2026
- Effective Date
- 01-Dec-2026
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Frequently Asked Questions
SIST EN ISO 10519:2026 is a standard published by the Slovenian Institute for Standardization (SIST). Its full title is "Rapeseed - Determination of chlorophyll content - Spectrometric method (ISO 10519:2026)". This standard covers: This document specifies a spectrometric method for the determination of the chlorophyll content of rapeseed. It is not applicable to the determination of chlorophyll in oils.
This document specifies a spectrometric method for the determination of the chlorophyll content of rapeseed. It is not applicable to the determination of chlorophyll in oils.
SIST EN ISO 10519:2026 is classified under the following ICS (International Classification for Standards) categories: 67.200.20 - Oilseeds. The ICS classification helps identify the subject area and facilitates finding related standards.
SIST EN ISO 10519:2026 has the following relationships with other standards: It is inter standard links to SIST EN ISO 10519:2015/A1:2025, SIST EN ISO 10519:2015. Understanding these relationships helps ensure you are using the most current and applicable version of the standard.
SIST EN ISO 10519:2026 is available in PDF format for immediate download after purchase. The document can be added to your cart and obtained through the secure checkout process. Digital delivery ensures instant access to the complete standard document.
Standards Content (Sample)
SLOVENSKI STANDARD
01-december-2026
Nadomešča:
SIST EN ISO 10519:2015
SIST EN ISO 10519:2015/A1:2025
Seme oljne repice - Določanje klorofila - Spektrometrijska metoda (ISO
10519:2026)
Rapeseed - Determination of chlorophyll content - Spectrometric method (ISO
10519:2026)
Rapssamen - Bestimmung des Chlorophyllgehaltes - Spektrometrisches Verfahren (ISO
10519:2026)
Graines de colza - Détermination de la teneur en chlorophylle - Méthode
spectrométrique (ISO 10519:2026)
Ta slovenski standard je istoveten z: EN ISO 10519:2026
ICS:
67.200.20 Oljnice Oilseeds
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.
EN ISO 10519
EUROPEAN STANDARD
NORME EUROPÉENNE
October 2026
EUROPÄISCHE NORM
ICS 67.200.20 Supersedes EN ISO 10519:2015,
EN ISO 10519:2015/A1:2025
English Version
Rapeseed - Determination of chlorophyll content -
Spectrometric method (ISO 10519:2026)
Graines de colza - Détermination de la teneur en Rapssamen - Bestimmung des Chlorophyllgehaltes -
chlorophylle - Méthode spectrométrique (ISO Spektrometrisches Verfahren (ISO 10519:2026)
10519:2026)
This European Standard was approved by CEN on 27 September 2026.
CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this
European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by
translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management
Centre has the same status as the official versions.
CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Türkiye and
United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATIO N
EUROPÄISCHES KOMITEE FÜR NORMUN G
CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2026 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 10519:2026 E
worldwide for CEN national Members.
Contents Page
European foreword . 3
European foreword
This document (EN ISO 10519:2026) has been prepared by Technical Committee ISO/TC 34 "Food
products" in collaboration with Technical Committee CEN/TC 307 “Oilseeds, vegetable and animal fats
and oils and their by-products - Methods of sampling and analysis” the secretariat of which is held by
AFNOR.
This European Standard shall be given the status of a national standard, either by publication of an
identical text or by endorsement, at the latest by April 2027, and conflicting national standards shall be
withdrawn at the latest by April 2027.
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. CEN shall not be held responsible for identifying any or all such patent rights.
This document supersedes EN ISO 10519:2015, EN ISO 10519:2015/A1:2025.
Any feedback and questions on this document should be directed to the users’ national standards
body/national committee. A complete listing of these bodies can be found on the CEN website.
According to the CEN-CENELEC Internal Regulations, the national standards organizations of the
following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria,
Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland,
Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Republic of
North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Türkiye and the
United Kingdom.
Endorsement notice
The text of ISO 10519:2026 has been approved by CEN as EN ISO 10519:2026 without any modification.
International
Standard
ISO 10519
Fourth edition
Rapeseed — Determination
2026-10
of chlorophyll content —
Spectrometric method
Graines de colza — Détermination de la teneur en chlorophylle —
Méthode spectrométrique
Reference number
ISO 10519:2026(en) © ISO 2026
ISO 10519:2026(en)
© ISO 2026
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may
be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting on
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Email: copyright@iso.org
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Published in Switzerland
ii
ISO 10519:2026(en)
Contents Page
Foreword .iv
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Principle . 1
5 Reagent . 1
6 Apparatus . 2
7 Sampling . 2
8 Preparation of test sample . 3
9 Procedure . 3
9.1 Test portions .3
9.2 Extraction .3
9.3 Determination .3
10 Expression of results . 4
11 Precision . 4
11.1 Repeatability .4
11.2 Reproducibility .4
12 Test report . 4
Annex A (informative) Results of interlaboratory test . 6
Annex B (informative) Preparation of the calibration curve to determine the k factor . 7
Bibliography . 9
iii
ISO 10519:2026(en)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards
bodies (ISO member bodies). The work of preparing International Standards is normally carried out through
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patent(s). ISO takes no position concerning the evidence, validity or applicability of any claimed patent
rights in respect thereof. As of the date of publication of this document, ISO had not received notice of (a)
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This document was prepared by Technical Committee ISO/TC 34, Food products, Subcommittee
SC 2, Oleaginous seeds and fruits and oilseed meals, in collaboration with the European Committee for
Standardization (CEN) Technical Committee CEN/TC 307, Oilseeds, vegetable and animal fats and oils and their
by-products - Methods of sampling and analysis, in accordance with the Agreement on technical cooperation
between ISO and CEN (Vienna Agreement).
This fourth edition cancels and replaces the third edition (ISO 10519:2015), of which it constitutes a minor
revision. It incorporates the Amendment ISO 10519:2015/Amd 1:2025.
The changes are as follows:
— Annex B “Preparation of the calibration curve to determine the k factor” has been added.
Any feedback or questions on this document should be directed to the user’s national standards body. A
complete listing of these bodies can be found at www.iso.org/members.html.
iv
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