SIST EN ISO 6888-2:2021/A1:2024
(Amendment)Microbiology of the food chain - Horizontal method for the enumeration of coagulase-positive staphylococci (Staphylococcus aureus and other species) - Part 2: Method using rabbit plasma fibrinogen agar medium - Amendment 1 (ISO 6888-2:2021/Amd 1:2023)
Microbiology of the food chain - Horizontal method for the enumeration of coagulase-positive staphylococci (Staphylococcus aureus and other species) - Part 2: Method using rabbit plasma fibrinogen agar medium - Amendment 1 (ISO 6888-2:2021/Amd 1:2023)
Mikrobiologie der Lebensmittelkette - Horizontales Verfahren für die Zählung von koagulase-positiven Staphylokokken (Staphylococcus aureus und andere Spezies) - Teil 2: Verfahren mit Kaninchenplasma-Fibrinogen-Agar-Medium - Änderung 1 (ISO 6888-2:2021/Amd 1:2023)
No scope available
Microbiologie de la chaîne alimentaire - Méthode horizontale pour le dénombrement des staphylocoques à coagulase positive (Staphylococcus aureus et autres espèces) - Partie 2: Méthode utilisant le milieu gélosé au plasma de lapin et au fibrinogène - Amendement 1 (ISO 6888-2:2021/Amd 1:2023)
Mikrobiologija v prehranski verigi - Horizontalna metoda za štetje koagulazno pozitivnih stafilokokov (Staphylococcus aureus in drugih vrst) - 2. del: Metoda uporabe agarja z zajčjo plazmo s fibrinogeni - Dopolnilo A1 (ISO 6888-2:2021/Amd 1:2023)
General Information
- Status
- Published
- Public Enquiry End Date
- 27-Feb-2023
- Publication Date
- 08-Feb-2024
- Technical Committee
- KŽP - Agricultural food products
- Current Stage
- 6060 - National Implementation/Publication (Adopted Project)
- Start Date
- 19-Dec-2023
- Due Date
- 23-Feb-2024
- Completion Date
- 09-Feb-2024
Relations
- Effective Date
- 01-Mar-2024
Overview
SIST EN ISO 6888-2:2021/A1:2024 is an important amendment to the international standard for microbiological analysis within the food chain. This document specifies a horizontal method for the enumeration of coagulase-positive staphylococci, including Staphylococcus aureus and other related species. Part 2 of this standard focuses on the method that employs rabbit plasma fibrinogen agar medium to detect and enumerate these bacteria.
The amendment, published by CEN in September 2023 and effective from March 2024, provides clarifications and updates that enhance the original ISO 6888-2:2021, ensuring improved accuracy and applicability in food microbiology testing. This standard aligns with international efforts to maintain food safety and hygiene, providing laboratories a reliable procedure to identify coagulase-positive staphylococci in various food products.
Key Topics
Enumeration of Coagulase-Positive Staphylococci: The standard details procedures for counting staphylococci that produce the enzyme coagulase, particularly Staphylococcus aureus, known for its role in foodborne illnesses.
Method Using Rabbit Plasma Fibrinogen Agar Medium: This specialized medium supports the growth and identification of coagulase-positive staphylococci by detecting fibrin clot formation resulting from coagulase activity.
Amendment Highlights:
- Enhanced suitability for fermented products and raw milk cheeses, which may contain complex microbiota including Staphylococcus xylosus.
- Consideration of background microbiota and atypical colony morphologies, which can affect detection accuracy on traditional Baird-Parker agar.
- Introduction of measurement uncertainty estimation in line with ISO 19036:2019 guidelines, improving quantitative testing reliability.
Reference Standard: The amendment incorporates ISO 19036:2019 for estimation of measurement uncertainty, promoting better quality control and laboratory consistency.
Applications
This standard serves crucial roles in:
Food Safety Testing: Monitoring coagulase-positive staphylococci in food products like dairy and meat to prevent staphylococcal food poisoning and ensure consumer safety.
Quality Control in Fermented Foods: Accurate enumeration supports the evaluation of products containing technological microbiota, such as certain cheeses and fermented meats, where Staphylococcus species contribute to product characteristics.
Clinical and Environmental Microbiology: Although food-focused, this method is relevant in broader contexts where reliable identification of coagulase-positive staphylococci is essential.
Regulatory Compliance: Enables laboratories to comply with national and international food safety regulations by providing standardized testing protocols.
Research and Development: Facilitates microbial studies related to food chain contamination, aiding in developing improved preservation techniques and microbial risk assessments.
Related Standards
ISO 6888 Series:
- Part 1 - Horizontal method for enumeration using Baird-Parker agar medium.
- Part 3 - Confirmation and identification of coagulase-positive staphylococci.
ISO 19036:2019 - Microbiology of the food chain - Estimation of measurement uncertainty for quantitative determinations, referenced for improving test result accuracy.
CEN/TC 463 Standards - Microbiology of the food chain, encompassing a range of methods for microbial detection and enumeration in food products.
ISO/IEC Directives Parts 1 and 2 - Guidelines on the development, maintenance, and editorial aspects of ISO standards, ensuring consistent quality and reliability.
By adopting SIST EN ISO 6888-2:2021/A1:2024, food microbiology laboratories contribute to enhanced safety of the food supply chain through standardized enumeration of coagulase-positive staphylococci. This ensures early detection of potential health hazards such as Staphylococcus aureus, supporting public health initiatives and fostering consumer confidence.
Frequently Asked Questions
SIST EN ISO 6888-2:2021/A1:2024 is a amendment published by the Slovenian Institute for Standardization (SIST). Its full title is "Microbiology of the food chain - Horizontal method for the enumeration of coagulase-positive staphylococci (Staphylococcus aureus and other species) - Part 2: Method using rabbit plasma fibrinogen agar medium - Amendment 1 (ISO 6888-2:2021/Amd 1:2023)". This standard covers: No scope available
No scope available
SIST EN ISO 6888-2:2021/A1:2024 is classified under the following ICS (International Classification for Standards) categories: 07.100.30 - Food microbiology. The ICS classification helps identify the subject area and facilitates finding related standards.
SIST EN ISO 6888-2:2021/A1:2024 has the following relationships with other standards: It is inter standard links to SIST EN ISO 6888-2:2021. Understanding these relationships helps ensure you are using the most current and applicable version of the standard.
You can purchase SIST EN ISO 6888-2:2021/A1:2024 directly from iTeh Standards. The document is available in PDF format and is delivered instantly after payment. Add the standard to your cart and complete the secure checkout process. iTeh Standards is an authorized distributor of SIST standards.
Standards Content (Sample)
SLOVENSKI STANDARD
01-marec-2024
Mikrobiologija v prehranski verigi - Horizontalna metoda za štetje koagulazno
pozitivnih stafilokokov (Staphylococcus aureus in drugih vrst) - 2. del: Metoda
uporabe agarja z zajčjo plazmo s fibrinogeni - Dopolnilo A1 (ISO 6888-2:2021/Amd
1:2023)
Microbiology of the food chain - Horizontal method for the enumeration of coagulase-
positive staphylococci (Staphylococcus aureus and other species) - Part 2: Method using
rabbit plasma fibrinogen agar medium - Amendment 1 (ISO 6888-2:2021/Amd 1:2023)
Mikrobiologie der Lebensmittelkette - Horizontales Verfahren für die Zählung von
koagulase-positiven Staphylokokken (Staphylococcus aureus und andere Spezies) - Teil
2: Verfahren mit Kaninchenplasma-Fibrinogen-Agar-Medium - Änderung 1 (ISO 6888-
2:2021/Amd 1:2023)
Microbiologie de la chaîne alimentaire - Méthode horizontale pour le dénombrement des
staphylocoques à coagulase positive (Staphylococcus aureus et autres espèces) - Partie
2: Méthode utilisant le milieu gélosé au plasma de lapin et au fibrinogène - Amendement
1 (ISO 6888-2:2021/Amd 1:2023)
Ta slovenski standard je istoveten z: EN ISO 6888-2:2021/A1:2023
ICS:
07.100.30 Mikrobiologija živil Food microbiology
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.
EN ISO 6888-2:2021/A1
EUROPEAN STANDARD
NORME EUROPÉENNE
September 2023
EUROPÄISCHE NORM
ICS 07.100.30
English Version
Microbiology of the food chain - Horizontal method for the
enumeration of coagulase-positive staphylococci
(Staphylococcus aureus and other species) - Part 2:
Method using rabbit plasma fibrinogen agar medium -
Amendment 1 (ISO 6888-2:2021/Amd 1:2023)
Microbiologie de la chaîne alimentaire - Méthode Mikrobiologie der Lebensmittelkette - Horizontales
horizontale pour le dénombrement des staphylocoques Verfahren für die Zählung von koagulase-positiven
à coagulase positive (Staphylococcus aureus et autres Staphylokokken (Staphylococcus aureus und andere
espèces) - Partie 2: Méthode utilisant le milieu gélosé Spezies) - Teil 2: Verfahren mit Kaninchenplasma-
au plasma de lapin et au fibrinogène - Amendement 1 Fibrinogen-Agar-Medium - Änderung 1 (ISO 6888-
(ISO 6888-2:2021/Amd 1:2023) 2:2021/Amd 1:2023)
This amendment A1 modifies the European Standard EN ISO 6888-2:2021; it was approved by CEN on 16 September 2023.
CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for inclusion of
this amendment into the relevant national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.
This amendment exists in three official versions (English, French, German). A version in any other language made by translation
under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management Centre has the
same status as the official versions.
CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Türkiye and
United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION
EUROPÄISCHES KOMITEE FÜR NORMUNG
CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2023 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 6888-2:2021/A1:2023 E
worldwide for CEN national Members.
Contents Page
European foreword . 3
European foreword
This document (EN ISO 6888-2:2021/A1:2023) has been prepared by Technical Committee ISO/TC 34
"Food products" in collaboration with Technical Committee CEN/TC 463 “Microbiology of the food
chain” the secretariat of which is held by AFNOR.
This Amendment to the European Standard EN ISO 6888-2:2021 shall be given the status of a national
standard, either by publication of an identical text or by endorsement, at the latest by March 2024, and
conflicting national standards shall be withdrawn at the latest by March 2024.
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. CEN shall not be held responsible for identifying any or all such patent rights.
Any feedback and questions on this document should be directed to the users’ national standards
body/national committee. A complete listing of these bodies can be found on the CEN website.
According to the CEN-CENELEC Internal Regulations, the national standards organizations of the
following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria,
Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland,
Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Republic of
North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Türkiye and the
United Kingdom.
Endorsement notice
The text of ISO 6888-2:2021/Amd 1:2023 has been approved by CEN as EN ISO 6888-2:2021/A1:2023
without any modification.
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