iTeh StandardsiTeh Standards
EURUSD
Your shopping cart is empty.
SIST

SIST EN ISO 21572:2013

(Main)

Foodstuffs - Molecular biomarker analysis - Protein-based methods (ISO 21572:2013)

Foodstuffs - Molecular biomarker analysis - Protein-based methods (ISO 21572:2013)

This International Standard provides general guidelines and performance criteria for methods for the detection and/or quantification of specific proteins or protein(s) of interest [POI(s)] in a specified matrix. These general guidelines address existing antibody based methods. Methods other than those described in Annex A or Annex B can also detect the POI. The same criteria as outlined in this International Standard apply generally.

Lebensmittel - Untersuchung von molekularen Biomarkern - Proteinverfahren (ISO 21572:2013)

Diese Internationale Norm legt allgemeine Leitlinien und Leistungskriterien für Verfahren zum Nachweis
und/oder zur quantitativen Bestimmung von spezifischem, aus gentechnisch verändertem (GV) pflanzlichen
Material stammenden Proteinen in einer spezifischen Matrix fest.
Diese allgemeinen Leitlinien gelten für vorhandene auf Antikörpern basierenden Verfahren. Auch andere Verfahren
als die im Anhang A oder Anhang B aufgeführten können Proteine nachweisen. Die in dieser Norm
umrissenen Kriterien werden allgemein angewendet.

Produits alimentaires - Analyse des biomarqueurs moléculaires - Méthodes basées sur les protéines (ISO 21572:2013)

L'ISO 21572:2013 énonce des lignes directrices et des critères de performances généraux pour les méthodes de détection et/ou de quantification de protéines spécifiques ou de protéine(s) d'intérêt [POI] dans une matrice donnée.
Ces lignes directrices générales concernent les méthodes basées sur des anticorps existants. Il existe d'autres méthodes que celles décrites en Annexe A ou B susceptibles de détecter des POI. Les mêmes critères que ceux répertoriés dans l'ISO 21572:2013 sont applicables de façon générale.

Živila - Analiza molekulskih biomarkerjev - Metode na osnovi proteinov (ISO 21572:2013)

Ta mednarodni standard zagotavlja splošne smernice in merila učinkovitosti za metode za odkrivanje in/ali kvantifikacijo posameznih zadevnih proteinov ali proteina(-ov) (POI) v določeni matrici. Te splošne smernice obravnavajo obstoječe metode na osnovi protiteles. Za odkrivanje zadevnih proteinov se lahko uporabljajo tudi metode, ki niso opisane v dodatku A ali B. Na splošno se uporabljajo merila iz tega mednarodnega standarda.

General Information

Status
Withdrawn
Public Enquiry End Date
14-Apr-2011
Publication Date
21-May-2013
Withdrawal Date
10-Dec-2019
ICS
67.050 - General methods of tests and analysis for food products
Technical Committee
KŽP - Agricultural food products
Current Stage
9900 - Withdrawal (Adopted Project)
Start Date
11-Dec-2019
Due Date
03-Jan-2020
Completion Date
11-Dec-2019
Ref Project
EN ISO 21572:2013 - Foodstuffs - Molecular biomarker analysis - Protein-based methods (ISO 21572:2013)

Relations

Replaces
SIST EN ISO 21572:2004 - Foodstuffs - Methods for the detection of genetically modified organisms and derived products - Protein based methods (ISO 21572:2004)
Effective Date
08-Jun-2022
Replaces
SIST EN ISO 21572:2004/AC:2005 - Foodstuffs - Methods for the detection of genetically modified organisms and derived products - Protein based methods (ISO 21572:2004)
Effective Date
01-Jun-2013
Replaces
SIST EN ISO 21572:2004 - Foodstuffs - Methods for the detection of genetically modified organisms and derived products - Protein based methods (ISO 21572:2004)
Effective Date
01-Jun-2013
Replaced By
SIST EN ISO 21572:2020 - Foodstuffs - Molecular biomarker analysis - Immunochemical methods for the detection and quantification of proteins (ISO 21572:2019)
Effective Date
08-Jun-2022
Replaced By
SIST EN ISO 21572:2020 - Foodstuffs - Molecular biomarker analysis - Immunochemical methods for the detection and quantification of proteins (ISO 21572:2019)
Effective Date
01-Jan-2020

Buy Standard

EN ISO 21572:2013EN ISO 21572:2013
PreviousNext
Standard
EN ISO 21572:2013
English language
36 pages
sale 10% off
Preview
sale 10% off
Preview
e-Library read for
1 day

Standards Content (Sample)

SLOVENSKI STANDARD
SIST EN ISO 21572:2013
01-junij-2013
1DGRPHãþD
SIST EN ISO 21572:2004
SIST EN ISO 21572:2004/AC:2005
Živila - Analiza molekulskih biomarkerjev - Metode na osnovi proteinov (ISO
21572:2013)
Foodstuffs - Molecular biomarker analysis - Protein-based methods (ISO 21572:2013)
Lebensmittel - Untersuchung von molekularen Biomarkern - Proteinverfahren (ISO
21572:2013)
Produits alimentaires - Analyse des biomarqueurs moléculaires - Méthodes basées sur
les protéines (ISO 21572:2013)
Ta slovenski standard je istoveten z: EN ISO 21572:2013
ICS:
67.050 Splošne preskusne in General methods of tests and
analizne metode za živilske analysis for food products
proizvode
SIST EN ISO 21572:2013 en
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

---------------------- Page: 1 ----------------------

SIST EN ISO 21572:2013

---------------------- Page: 2 ----------------------

SIST EN ISO 21572:2013


EUROPEAN STANDARD
EN ISO 21572

NORME EUROPÉENNE

EUROPÄISCHE NORM
February 2013
ICS 67.050 Supersedes EN ISO 21572:2004
English Version
Foodstuffs - Molecular biomarker analysis - Protein-based
methods (ISO 21572:2013)
Produits alimentaires - Analyse des biomarqueurs Lebensmittel - Untersuchung von molekularen Biomarkern -
moléculaires - Méthodes basées sur les protéines (ISO Proteinverfahren (ISO 21572:2013)
21572:2013)
This European Standard was approved by CEN on 12 January 2013.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European
Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national
standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN member.

This European Standard exists in three official versions (English, French, German). A version in any other language made by translation
under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management Centre has the same
status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania,
Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and United
Kingdom.





EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

Management Centre: Avenue Marnix 17, B-1000 Brussels
© 2013 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 21572:2013: E
worldwide for CEN national Members.

---------------------- Page: 3 ----------------------

SIST EN ISO 21572:2013
EN ISO 21572:2013 (E)
Contents Page
Foreword .3
2

---------------------- Page: 4 ----------------------

SIST EN ISO 21572:2013
EN ISO 21572:2013 (E)
Foreword
This document (EN ISO 21572:2013) has been prepared by Technical Committee ISO/TC 34 "Food products"
in collaboration with Technical Committee CEN/TC 275 “Food analysis - Horizontal methods” the secretariat of
which is held by DIN.
This European Standard shall be given the status of a national standard, either by publication of an identical
text or by endorsement, at the latest by August 2013, and conflicting national standards shall be withdrawn at
the latest by August 2013.
Attention is drawn to the possibility that some of the elements of this document may be the subject of patent
rights. CEN [and/or CENELEC] shall not be held responsible for identifying any or all such patent rights.
This document supersedes EN ISO 21572:2004.
According to the CEN-CENELEC Internal Regulations, the national standards organizations of the following
countries are bound to implement this European Standard: Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech
Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece,
Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal,
Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and the United Kingdom.
Endorsement notice
The text of ISO 21572:2013 has been approved by CEN as EN ISO 21572:2013 without any modification.

3

---------------------- Page: 5 ----------------------

SIST EN ISO 21572:2013

---------------------- Page: 6 ----------------------

SIST EN ISO 21572:2013
INTERNATIONAL ISO
STANDARD 21572
Second edition
2013-02-01
Foodstuffs — Molecular biomarker
analysis — Protein-based methods
Produits
...

Questions, Comments and Discussion

Ask us and Technical Secretary will try to provide an answer. You can facilitate discussion about the standard in here.

This May Also Interest You

SIST
SIST EN 17851:2023(Main)
Foodstuffs - Determination of elements and their chemical species - Determination of Ag, As, Cd, Co, Cr, Cu, Mn, Mo, Ni, Pb, Se, Tl, U and Zn in foodstuffs by inductively coupled plasma mass spectrometry (ICP-MS) after pressure digestion
EN 17851:2023

This document specifies a procedure for the determination of Ag, As, Cd, Co, Cr, Cu, Mn, Mo, Ni, Pb, Se, Tl,
U and Zn in foodstuffs by inductively coupled plasma mass spectrometry (ICP-MS) after pressure
digestion.
The following foodstuffs were analysed for the elements listed in Table 1 in an interlaboratory study:
Banana (deep-frozen), Cocoa powder, Wheat noodle powder, Currant nectar (deep-frozen), Milk powder,
Oyster (dried), Celery (dried), Dogfish liver (dried), Liver (deep-frozen), Kale (dried).

  • Standard
    39 pages
    English language
    sale 10% off
    e-Library read for
    1 day
SIST
SIST EN ISO 20813:2023(Main)
Molecular biomarker analysis - Methods of analysis for the detection and identification of animal species in foods and food products (nucleic acid-based methods) - General requirements and definitions (ISO 20813:2019)
EN ISO 20813:2022

This document specifies minimum requirements of performance characteristics for the detection of nucleic acid sequences (DNA) by molecular methods, such as the polymerase chain reaction (PCR), including different post-PCR detection methods, real-time PCR, single and/or multiple probe-based detection techniques as well as the combination of such methods.
The document is applicable to the detection, identification and quantification of DNA from animal species of higher and lower taxonomic groups in foodstuffs, and the validation of applicable methods.
It is applicable to mammals, birds, reptiles, amphibians, fishes, molluscs, crustaceans and insects. Typical examples for each are listed in Annex A.

  • Standard
    34 pages
    English language
    sale 10% off
    e-Library read for
    1 day
SIST
SIST EN 15634-3:2023(Main)
Foodstuffs - Detection of food allergens by molecular biological methods - Part 3: Hazelnut (Corylus avellana) - Qualitative detection of a specific DNA sequence in chocolate by real-time PCR
EN 15634-3:2023

This method describes a procedure for the qualitative detection of hazelnut (Corylus avellana) in
chocolate. DNA is extracted from the chocolate and a specific DNA sequence for hazelnut
detected from the gene for corA 1.

  • Standard
    16 pages
    English language
    sale 10% off
    e-Library read for
    1 day
SIST
SIST EN 15634-5:2023(Main)
Foodstuffs - Detection of food allergens by molecular biological methods - Part 5: Mustard (Sinapis alba) and soya (Glycine max) - Qualitative detection of a specific DNA sequence in cooked sausages by real-time PCR
EN 15634-5:2023

This method specifies a procedure for the qualitative detection of species specific DNA from
white mustard (Sinapis alba) and soya (Glycine max) in cooked sausages using singleplex realtime
PCR based on the genes MADS-D (mustard) and lectin (soya). A mustard content of 10
mg/kg or greater and a soya content of 10 mg/kg or greater can be detected with a probability of
> 95 %.

  • Standard
    17 pages
    English language
    sale 10% off
    e-Library read for
    1 day
SIST
SIST EN 15634-4:2023(Main)
Foodstuffs - Detection of food allergens by molecular biological methods - Part 4: Peanut (Arachis hypogaea) - Qualitative detection of a specific DNA sequence in chocolate by real-time PCR
EN 15634-4:2023

This method describes a procedure for the qualitative detection of peanut (Arachis hypogaea) in
chocolate using real-time PCR based on the gene for the peanut allergen Ara h 2 [4, 5].

  • Standard
    16 pages
    English language
    sale 10% off
    e-Library read for
    1 day
SIST
SIST EN ISO 22753:2023(Main)
Molecular biomarker analysis - Method for the statistical evaluation of analytical results obtained in testing sub-sampled groups of genetically modified seeds and grains - General requirements (ISO 22753:2021, Corrected version 2022-11)
EN ISO 22753:2022

This document describes general requirements, procedures and performance criteria for evaluating the content of genetically modified (GM) seeds/grains in a lot by a group testing strategy that includes qualitative analysis of sub-sampled groups followed by statistical evaluation of the results.
This document is applicable to group testing strategy estimating the GM content on a percentage seed/grain basis for purity estimation, testing towards a given reject/accept criterion and for cases where seed/grain lots are carrying stacked events.
This document is not applicable to processed products.
NOTE       Description of the use of group testing strategy are available in References [1], [7], [8], [18], [19] and [20].

  • Standard
    34 pages
    English language
    sale 10% off
    e-Library read for
    1 day
SIST
SIST EN 17641:2022(Main)
Foodstuffs - Multimethod for the determination of aflatoxins, deoxynivalenol, fumonisins, ochratoxin A, T-2 toxin, HT-2 toxin and zearalenone by LC-MS/MS
EN 17641:2022

This document describes an isotope dilution method for the quantitative determination of aflatoxins B1, B2, G1, G2 and M1 (AFB1, AFB2, AFG1, AFG2 and AFM1), ochratoxin A (OTA), deoxynivalenol (DON), zearalenone (ZEN), T-2 and HT-2 toxins (T-2 and HT-2) and fumonisins B1 and B2 (FB1 and FB2) in foods by liquid chromatography (LC) coupled with tandem mass spectrometry (MS/MS).
A specific immunoaffinity column (IAC) clean-up is needed for aflatoxins (AFs) and OTA in infant foods (e.g. infant cereals, milk-based powders), in spices, in dried fruits and in nuts.
The method has been validated through an intercollaborative study on different commodity groups: cereals and cereal-based products including food for infant and young children, nuts, spices, dried fruits and milk powder. The ranges of concentrations of each mycotoxin in these naturally contaminated and/or spiked food samples were:
-   aflatoxin B1:   0,0857 µg/kg - 11,4 µg/kg;
-   aflatoxin B2:   0,0792 µg/kg - 12,5 µg/kg;
-   aflatoxin G1:   0,0628 µg/kg - 20,9 µg/kg;
-   aflatoxin G2:   0,0520 µg/kg - 15,0 µg/kg;
-   aflatoxin M1:   0,0342 µg/kg - 0,110 µg/kg;
-   ochratoxin A:   0,448 µg/kg - 17,2 µg/kg;
-   deoxynivalenol:   45,2 µg/kg - 743 µg/kg;
-   zearalenone:   9,57 µg/kg - 131 µg/kg;
-   T-2 toxin:      10,3 µg/kg - 57,9 µg/kg;
-   HT-2 toxin:   9,50 µg/kg - 81,8 µg/kg;
-   fumonisin B1:   31,1 µg/kg - 4 262 µg/kg;
-   fumonisin B2:   44,2 µg/kg - 1 299 µg/kg.
The measuring ranges of the method for each mycotoxin/matrix combination are given in Table 7.

  • Standard
    49 pages
    English language
    sale 10% off
    e-Library read for
    1 day
SIST
SIST EN 17644:2022(Main)
Foodstuffs - Detection of food allergens by liquid chromatography - mass spectrometry (LC-MS) methods - General considerations
EN 17644:2022

This document establishes an overall framework covering qualitative and quantitative methods for the determination of food allergens and allergenic ingredients using mass spectrometry-based methods for the determination of specific peptides/proteins. This document provides general guidelines and performance criteria applicable to this methodology. Guidelines, minimum requirements and performance criteria laid down in this document are intended to ensure that comparable and reproducible results are obtained by different analysts, instrumentation and laboratories.

  • Standard
    20 pages
    English language
    sale 10% off
    e-Library read for
    1 day
SIST
SIST EN 13708:2022(Main)
Foodstuffs - Detection of irradiated foodstuff containing crystalline sugar by ESR spectroscopy
EN 13708:2022

This European Standard specifies a method for the detection of foods containing crystalline sugars which have been treated with ionizing radiation, by analysing the electron spin resonance (ESR) spectrum, also called electron paramagnetic resonance (EPR) spectrum, of the food.
Interlaboratory studies have been successfully carried out on dried figs, dried mangoes, dried papayas and raisins.

  • Standard
    12 pages
    English language
    sale 10% off
    e-Library read for
    1 day
SIST
SIST EN 1787:2022(Main)
Foodstuff - Detection of irradiated foodstuff containing cellulose by ESR spectroscopy
EN 1787:2022

This European Standard specifies a method for the detection of foods containing cellulose which have been treated with ionizing radiation, by analysing the electron spin resonance (ESR) spectrum, also called electron paramagnetic resonance (EPR) spectrum, of the food.
Interlaboratory studies have been successfully carried out with pistachio nut shells, paprika powder and fresh strawberries. However, it has been shown that false positive results can appear when analysing bleached nuts. For further information, see Clause 7 on limitations.

  • Standard
    16 pages
    English language
    sale 10% off
    e-Library read for
    1 day