Animal and vegetable fats and oils - Determination of anisidine value (ISO 6885:2016)

ISO 6885:2016 specifies a method for the determination of the anisidine value in animal and vegetable fats and oils. This is a measure of the amount of aldehydes present (principally α, β-unsaturated aldehydes).
Milk and milk products (or fat coming from milk and milk products) are excluded from the scope of this International Standard.

Tierische und pflanzliche Fette und Öle - Bestimmung der Anisidinzahl (ISO 6885:2016)

Diese Internationale Norm legt ein Verfahren zur Bestimmung der Anisidinzahl in tierischen und pflanzlichen Fetten und Ölen fest. Diese ist ein Maß für die Menge an vorhandenen Aldehyden (vorwiegend α, β-unge¬sättigte Aldehyde).
Milch und Milcherzeugnisse (oder Fett aus Milch und Milcherzeugnissen) sind vom Anwendungsbereich dieser Internationalen Norm ausgenommen.

Corps gras d'origines animale et végétale - Détermination de l'indice d'anisidine (ISO 6885:2016)

ISO 6885:2016 spécifie une méthode de détermination de l'indice d'anisidine qui correspond à un mesurage de la quantité d'aldéhydes (principalement des aldéhydes α, β insaturés) présents dans les corps gras d'origines animale et végétale.
Le lait et les produits laitiers (ou les corps gras issus du lait et des produits laitiers) sont exclus du domaine d'application de la présente Norme internationale.

Rastlinske in živalske maščobe in olja - Določevanje anisidinskega števila (ISO 6885:2016)

Ta mednarodni standard določa metodo za določevanje anisidinske vrednosti rastlinskih in živalskih maščob ter olj. To je meritev količine prisotnih aldehidov (v glavnem α, β-nenasičenih aldehidov). Mleko in mlečni izdelki (ali maščoba iz mleka in mlečnih izdelkov) niso zajeti v tem mednarodnem standardu.

General Information

Status
Published
Publication Date
10-Apr-2016
Current Stage
6060 - National Implementation/Publication (Adopted Project)
Start Date
23-Mar-2016
Due Date
28-May-2016
Completion Date
11-Apr-2016

Relations

Overview

EN ISO 6885:2016 (ISO 6885:2016) specifies a standardized chemical method for the determination of the anisidine value in animal and vegetable fats and oils. The anisidine value quantifies aldehydes (principally α,β‑unsaturated aldehydes) formed during lipid oxidation and is reported as 100 times the increase in absorbance at 350 nm (10 mm cell) for a defined test solution. Milk and milk products (and fats from milk) are explicitly excluded from the scope.

Key topics and technical requirements

  • Principle: Reaction of the oil/fat test solution in isooctane with p‑anisidine (in glacial acetic acid) and measurement of the increase in absorbance at 350 nm.
  • Reagents & safety: Use analytical‑grade reagents; p‑anisidine is toxic and must be handled with appropriate precautions and stored refrigerated in the dark. Isooctane with low UV absorbance is required.
  • Apparatus: Single‑ or double‑beam spectrometer capable of 350 nm measurements using 10 mm cells; matched cells recommended for double‑beam.
  • Sample preparation: Follow ISO 661 for preparation of test sample; dry samples >0.10% moisture with anhydrous sodium sulfate. Typical test portions range 0.4 g to 4.0 g in a 25 ml volumetric flask.
  • Procedure controls: Reaction times and temperatures are specified (reaction ~10 min at (23 ± 3) °C); blank and unreacted controls are required. The anisidine value calculation and expression are standardized.
  • Precision and validation: The standard includes repeatability/reproducibility data from interlaboratory testing and reporting requirements.
  • Normative references: ISO 661, ISO 3696 and (sampling guidance) ISO 5555 are referenced.

Applications and users

  • Quality control in edible oil, fat and oilseed processing: monitor secondary oxidation products and rancidity.
  • Shelf‑life and storage testing: assess accumulation of aldehydes during storage or processing.
  • Regulatory and compliance testing: standardized reporting for trade and certification in the food industry.
  • Research and formulation: evaluate antioxidant performance, refining processes, and product stability.
  • Typical users: food testing laboratories, oil refiners, edible oil manufacturers, certification bodies, and academic researchers.

Related standards

  • ISO 661 - Preparation of test sample (fats & oils)
  • ISO 3696 - Water for analytical laboratory use
  • ISO 5555 - Sampling of animal and vegetable fats and oils (recommended)
  • Supersedes: EN ISO 6885:2007 (updated technically in 2016)

This standard is essential for consistent, comparable measurement of aldehyde content in fats and oils and is frequently referenced in oil quality assessment, rancidity monitoring and product specification testing.

Standard
SIST EN ISO 6885:2016
English language
14 pages
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Standards Content (Sample)


SLOVENSKI STANDARD
01-maj-2016
1DGRPHãþD
SIST EN ISO 6885:2007
5DVWOLQVNHLQåLYDOVNHPDãþREHLQROMD'RORþHYDQMHDQLVLGLQVNHJDãWHYLOD ,62

Animal and vegetable fats and oils - Determination of anisidine value (ISO 6885:2016)
Tierische und pflanzliche Fette und Öle - Bestimmung der Anisidinzahl (ISO 6885:2016)
Corps gras d'origines animale et végétale - Détermination de l'indice d'anisidine (ISO
6885:2016)
Ta slovenski standard je istoveten z: EN ISO 6885:2016
ICS:
67.200.10 5DVWOLQVNHLQåLYDOVNH Animal and vegetable fats
PDãþREHLQROMD and oils
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

EN ISO 6885
EUROPEAN STANDARD
NORME EUROPÉENNE
March 2016
EUROPÄISCHE NORM
ICS 67.200.10 Supersedes EN ISO 6885:2007
English Version
Animal and vegetable fats and oils - Determination of
anisidine value (ISO 6885:2016)
Corps gras d'origines animale et végétale - Tierische und pflanzliche Fette und Öle - Bestimmung
Détermination de l'indice d'anisidine (ISO 6885:2016) der Anisidinzahl (ISO 6885:2016)
This European Standard was approved by CEN on 30 January 2016.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this
European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by
translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management
Centre has the same status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania,
Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and
United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

CEN-CENELEC Management Centre: Avenue Marnix 17, B-1000 Brussels
© 2016 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 6885:2016 E
worldwide for CEN national Members.

Contents Page
European foreword . 3
European foreword
This document (EN ISO 6885:2016) has been prepared by Technical Committee ISO/TC 34 “Food
products” in collaboration with Technical Committee CEN/TC 307 “Oilseeds, vegetable and animal fats
and oils and their by-products - Methods of sampling and analysis” the secretariat of which is held by
AFNOR.
This European Standard shall be given the status of a national standard, either by publication of an
identical text or by endorsement, at the latest by September 2016, and conflicting national standards
shall be withdrawn at the latest by September 2016.
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. CEN [and/or CENELEC] shall not be held responsible for identifying any or all such patent
rights.
This document supersedes EN ISO 6885:2007.
According to the CEN-CENELEC Internal Regulations, the national standards organizations of the
following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria,
Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia,
France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta,
Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland,
Turkey and the United Kingdom.
Endorsement notice
The text of ISO 6885:2016 has been approved by CEN as EN ISO 6885:2016 without any modification.

INTERNATIONAL ISO
STANDARD 6885
Fourth edition
2016-02-15
Animal and vegetable fats and oils —
Determination of anisidine value
Corps gras d’origines animale et végétale — Détermination de
l’indice d’anisidine
Reference number
ISO 6885:2016(E)
©
ISO 2016
ISO 6885:2016(E)
© ISO 2016, Published in Switzerland
All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized otherwise in any form
or by any means, electronic or mechanical, including photocopying, or posting on the internet or an intranet, without prior
written permission. Permission can be requested from either ISO at the address below or ISO’s member body in the country of
the requester.
ISO copyright office
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CH-1214 Vernier, Geneva, Switzerland
Tel. +41 22 749 01 11
Fax +41 22 749 09 47
copyright@iso.org
www.iso.org
ii © ISO 2016 – All rights reserved

ISO 6885:2016(E)
Contents Page
Foreword .iv
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Principle . 1
5 Reagents . 1
6 Apparatus . 2
7 Sampling . 2
8 Preparation of test sample . 2
9 Procedure. 3
9.1 Test portion and preparation of test solution . 3
9.2 Unreacted test solution . 3
9.3 Reacted test solution . 3
9.4 Blank . 3
9.5 Spectrometric measurement . 3
9.6 Absorbance range . 3
10 Expression of results . 4
11 Precision . 4
11.1 Interlaboratory tests . 4
11.2 Repeatability . 4
11.3 Reproducibility . 4
12 Test report . 5
Annex A (informative) Results of the interlaboratory test . 6
Bibliography . 7
ISO 6885:2016(E)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards
bodies (ISO member bodies). The work of preparing International Standards is normally carried out
through ISO technical committees. Each member body interested in a subject for which a technical
committee has been established has the right to be represented on that committee. International
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ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of
electrotechnical standardization.
The procedures used to develop this document and those intended for its further maintenance are
described in the ISO/IEC Directives, Part 1. In particular the different approval criteria needed for the
different types of ISO documents should be noted. This document was drafted in accordance with the
editorial rules of the ISO/IEC Directives, Part 2 (see www.iso.org/directives).
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. ISO shall not be held responsible for identifying any or all such patent rights. Details of
any patent rights identified during the development of the document will be in the Introduction and/or
on the ISO list of patent declarations received (see www.iso.org/patents).
Any trade name used in this document is information given for the convenience of users and does not
constitute an endorsement.
For an explanation on the meaning of ISO specific terms and expressions related to conformity
assessment, as well as information about ISO’s adherence to the WTO principles in the Technical
Barriers to Trade (TBT) see the following URL: F
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Frequently Asked Questions

SIST EN ISO 6885:2016 is a standard published by the Slovenian Institute for Standardization (SIST). Its full title is "Animal and vegetable fats and oils - Determination of anisidine value (ISO 6885:2016)". This standard covers: ISO 6885:2016 specifies a method for the determination of the anisidine value in animal and vegetable fats and oils. This is a measure of the amount of aldehydes present (principally α, β-unsaturated aldehydes). Milk and milk products (or fat coming from milk and milk products) are excluded from the scope of this International Standard.

ISO 6885:2016 specifies a method for the determination of the anisidine value in animal and vegetable fats and oils. This is a measure of the amount of aldehydes present (principally α, β-unsaturated aldehydes). Milk and milk products (or fat coming from milk and milk products) are excluded from the scope of this International Standard.

SIST EN ISO 6885:2016 is classified under the following ICS (International Classification for Standards) categories: 67.200.10 - Animal and vegetable fats and oils. The ICS classification helps identify the subject area and facilitates finding related standards.

SIST EN ISO 6885:2016 has the following relationships with other standards: It is inter standard links to SIST EN ISO 6885:2007. Understanding these relationships helps ensure you are using the most current and applicable version of the standard.

You can purchase SIST EN ISO 6885:2016 directly from iTeh Standards. The document is available in PDF format and is delivered instantly after payment. Add the standard to your cart and complete the secure checkout process. iTeh Standards is an authorized distributor of SIST standards.