Animal feeding stuffs - Determination of semduramicin content - Liquid chromatographic method using a "tree" analytical approach

This European standard specifies a high-performance liquid chromatographic (HPLC) method for the determination of the semduramicin content at authorized level in animal feeding stuffs [2], using mass spectrometry detection or post-column derivatization and (UV)-VIS detection (hereinafter UV detection). This method is applicable to poultry feed. The limit of quantitation is 1,0 mg/kg when mass spectrometry is used for detection and 3,0 mg/kg when the detection is performed by UV with post-column derivatization. Lower limits of quantitation are achievable but this is to be validated by the user. The method allows the discrimination of semduramicin from monensin, salinomycin, narasin, maduramicin and lasalocid.

Futtermittel - Bestimmung des Semduramicingehalts - Flüssigkeitschromatographisches Verfahren mit verzweigter analytischer Vorgehensweise

Die vorliegende Internationale Norm legt ein hochleistungsflüssigkeitschromatographisches (HPLC) Verfahren zur Bestimmung des Semduramicingehaltes bei einem zugelassenen Gehalt in Futtermitteln mittels massen-spektrometrischer Detektion oder Nachsäulenderivatisierung und UV Detektion fest. Das Verfahren gilt für Geflügelfutter. Bei der Anwendung von Massenspektrometrie zum Nachweis beträgt die Bestimmungsgrenze 1,0 mg  kg–1 und beim Nachweis mittels UV mit Nachsäulenderivatisierung 3,0 mg  kg 1. Niedrigere Bestimmungsgrenzen sind erreichbar, das ist jedoch vom Anwender zu validieren.
Das Verfahren ermöglicht es, Semduramicin von Monensin, Salinomycin, Narasin, Maduramicin und Lasalocid zu unterscheiden.

Aliments pour animaux - Dosage de la semduramicine - Chromatographie liquide utilisant une approche analytique en arbre

La présente Norme internationale décrit une méthode de dosage de la semduramicine au taux autorisé dans les aliments pour animaux, par chromatographie liquide haute performance (CLHP) avec détection par spectrométrie de masse ou dérivation post-colonne et détection UV. Cette méthode est applicable aux aliments pour volailles. La limite de quantification est de 1,0 mg kg-1 avec détection par spectrométrie de masse et de 3,0 mg kg-1 avec détection UV et dérivation post-colonne. Des limites de quantification inférieures peuvent être obtenues, à condition qu'elles soient validées par l'utilisateur.
La méthode permet de différencier la semduramicine du monensine, de la salinomycine, du narasine, de la maduramicine et du lasalocide.

Krma - Določevanje semduramicina - Metoda tekočinske kromatografije z "razvejanim" analitskim pristopom

Ta evropski standard določa metodo tekočinske kromatografije visoke ločljivosti (HPLC) za določevanje dovoljene vrednosti vsebnosti semduramicina v krmi [2] z masno spektrometrijo ali postkolonsko derivatizacijo in zaznavanjem (UV)-VIS (v nadaljnjem besedilu: zaznavanje UV). Ta metoda se uporablja za perutninsko krmo. Če se za zaznavanje uporablja masna spektrometrija, je meja kvantifikacije 1,0 mg/kg, če pa se zaznavanje izvaja z zaznavanjem UV s postkolonsko derivatizacijo, pa 3,0 mg/kg. Nižje meje kvantifikacije so dosegljive, vendar jih mora validirati uporabnik. Metoda omogoča razlikovanje semduramicina od monenzina, salinomicina, narazina, maduramicina in lasalocida.

General Information

Status
Published
Public Enquiry End Date
14-Feb-2011
Publication Date
12-Mar-2012
Current Stage
6060 - National Implementation/Publication (Adopted Project)
Start Date
01-Mar-2012
Due Date
06-May-2012
Completion Date
13-Mar-2012

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Standards Content (Sample)

2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.Futtermittel - Bestimmung des Semduramicingehalts - Flüssigkeitschromatographisches Verfahren mit verzweigter analytischer VorgehensweiseAliments pour animaux - Dosage de la semduramicine - Chromatographie liquide utilisant une approche analytique en arbreAnimal feeding stuffs - Determination of semduramicin content - Liquid chromatographic method using a "tree" analytical approach65.120KrmilaAnimal feeding stuffsICS:Ta slovenski standard je istoveten z:EN 16158:2012SIST EN 16158:2012en,fr,de01-april-2012SIST EN 16158:2012SLOVENSKI
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SIST EN 16158:2012



EUROPEAN STANDARD NORME EUROPÉENNE EUROPÄISCHE NORM
EN 16158
February 2012 ICS 65.120 English Version
Animal feeding stuffs - Determination of semduramicin content -Liquid chromatographic method using a "tree" analytical approach
Aliments pour animaux - Dosage de la semduramicine - Chromatographie liquide utilisant une approche analytique en arbre
Futtermittel - Bestimmung des Semduramicingehalts - Flüssigkeitschromatographisches Verfahren mit verzweigter analytischer Vorgehensweise This European Standard was approved by CEN on 30 December 2011.
CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management Centre has the same status as the official versions.
CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION EUROPÄISCHES KOMITEE FÜR NORMUNG
Management Centre:
Avenue Marnix 17,
B-1000 Brussels © 2012 CEN All rights of exploitation in any form and by any means reserved worldwide for CEN national Members. Ref. No. EN 16158:2012: ESIST EN 16158:2012



EN 16158:2012 (E) 2 Contents Page Foreword .31Scope .42Normative references .43Principle .44Reagents .45Apparatus .76Sampling .87Preparation of test sample .87.1General .87.2Laboratory sample .97.3Test sample .97.4Test portion .98Procedure .98.1Preparation of positive and negative control samples .98.2Samples extraction .98.3Filtration .
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