SIST EN ISO 6887-1:2017/A1:2024
(Amendment)Microbiology of the food chain - Preparation of test samples, initial suspension and decimal dilutions for microbiological examination - Part 1: General rules for the preparation of the initial suspension and decimal dilutions - Amendment 1: Requirements and guidance on the use of larger test portion size for qualitative method (ISO 6887-1:2017/Amd 1:2024)
Microbiology of the food chain - Preparation of test samples, initial suspension and decimal dilutions for microbiological examination - Part 1: General rules for the preparation of the initial suspension and decimal dilutions - Amendment 1: Requirements and guidance on the use of larger test portion size for qualitative method (ISO 6887-1:2017/Amd 1:2024)
Mikrobiologie der Lebensmittelkette - Vorbereitung von Untersuchungsproben und Herstellung von Erstverdünnungen und von Dezimalverdünnungen für mikrobiologische Untersuchungen - Teil 1: Allgemeine Regeln für die Herstellung von Erstverdünnungen und Dezimalverdünnungen - Änderung 1: Anforderungen und Leitlinien für die Verwendung größerer Prüfmengen bei qualitativen Verfahren (ISO 6887-1:2017/Amd 1:2024)
Microbiologie de la chaîne alimentaire - Préparation des échantillons, de la suspension mère et des dilutions décimales en vue de l'examen microbiologique - Partie 1: Règles générales pour la préparation de la suspension mère et des dilutions décimales - Amendement 1: Exigences et recommandations sur l’utilisation d’une taille de prise d’essai plus grande pour les méthodes qualitatives (ISO 6887-1:2017/Amd 1:2024)
Mikrobiologija v prehranski verigi - Priprava preskusnih vzorcev, osnovne suspenzije in decimalnih razredčin za mikrobiološko preiskavo - 1. del: Splošna pravila za pripravo osnovne suspenzije in decimalnih razredčin - Dopolnilo A1: Zahteve in navodila o uporabi testnega vzorca večje velikosti za kvalitativne metode (ISO 6887-1:2017/Amd 1:2024)
General Information
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Standards Content (Sample)
SLOVENSKI STANDARD
SIST EN ISO 6887-1:2017/oprA1:2023
01-oktober-2023
Mikrobiologija v prehranski verigi - Priprava preskusnih vzorcev, osnovne
suspenzije in decimalnih razredčin za mikrobiološko preiskavo - 1. del: Splošna
pravila za pripravo osnovne suspenzije in decimalnih razredčin - Dopolnilo A1:
Zahteve in navodila o uporabi testnega vzorca večje velikosti za kvalitativne
metode (ISO 6887-1:2017/DAM1:2023)
Microbiology of the food chain - Preparation of test samples, initial suspension and
decimal dilutions for microbiological examination - Part 1: General rules for the
preparation of the initial suspension and decimal dilutions - Amendment 1: Requirements
and guidance on the use of larger test portion size for qualitative methods (ISO 6887-
1:2017/DAM1:2023)
Mikrobiologie der Lebensmittelkette - Vorbereitung von Untersuchungsproben und
Herstellung von Erstverdünnungen und von Dezimalverdünnungen für mikrobiologische
Untersuchungen - Teil 1: Allgemeine Regeln für die Herstellung von Erstverdünnungen
und Dezimalverdünnungen - Änderung 1: Anforderungen und Leitlinien für die
Verwendung größerer Prüfmengen bei qualitativen Verfahren (ISO 6887 1:2017/DAM
1:2023)
Microbiologie de la chaîne alimentaire - Préparation des échantillons, de la suspension
mère et des dilutions décimales en vue de l'examen microbiologique - Partie 1: Règles
générales pour la préparation de la suspension mère et des dilutions décimales -
Amendement 1: Exigences et recommandations sur l’utilisation d’une taille de prise
d’essai plus grande pour les méthodes qualitatives (ISO 6887-1:2017/DAM1:2023)
Ta slovenski standard je istoveten z: EN ISO 6887-1:2017/prA1
ICS:
07.100.30 Mikrobiologija živil Food microbiology
SIST EN ISO 6887-1:2017/oprA1:2023 en,fr,de
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.
SIST EN ISO 6887-1:2017/oprA1:2023
SIST EN ISO 6887-1:2017/oprA1:2023
DRAFT AMENDMENT
ISO 6887-1:2017/DAM 1
ISO/TC 34/SC 9 Secretariat: AFNOR
Voting begins on: Voting terminates on:
2023-07-31 2023-10-23
Microbiology of the food chain — Preparation of test
samples, initial suspension and decimal dilutions for
microbiological examination —
Part 1:
General rules for the preparation of the initial suspension
and decimal dilutions
AMENDMENT 1: Requirements and guidance on the use of
larger test portion size for qualitative methods
ICS: 07.100.30
This document is circulated as received from the committee secretariat.
THIS DOCUMENT IS A DRAFT CIRCULATED
FOR COMMENT AND APPROVAL. IT IS
ISO/CEN PARALLEL PROCESSING
THEREFORE SUBJECT TO CHANGE AND MAY
NOT BE REFERRED TO AS AN INTERNATIONAL
STANDARD UNTIL PUBLISHED AS SUCH.
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NATIONAL REGULATIONS.
ISO 6887-1:2017/DAM 1:2023(E)
RECIPIENTS OF THIS DRAFT ARE INVITED
TO SUBMIT, WITH THEIR COMMENTS,
NOTIFICATION OF ANY RELEVANT PATENT
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PROVIDE SUPPORTING DOCUMENTATION. © ISO 2023
SIST EN ISO 6887-1:2017/oprA1:2023
ISO 6887-1:2017/DAM 1:2023(E)
DRAFT AMENDMENT
ISO 6887-1:2017/DAM 1
ISO/TC 34/SC 9 Secretariat: AFNOR
Voting begins on: Voting terminates on:
Microbiology of the food chain — Preparation of test
samples, initial suspension and decimal dilutions for
microbiological examination —
Part 1:
General rules for the preparation of the initial suspension
and decimal dilutions
AMENDMENT 1: Requirements and guidance on the use of
larger test portion size for qualitative methods
ICS: 07.100.30
This document is circulated as received from the committee secretariat.
THIS DOCUMENT IS A DRAFT CIRCULATED
FOR COMMENT AND APPROVAL. IT IS
© ISO 2023
ISO/CEN PARALLEL PROCESSING
THEREFORE SUBJECT TO CHANGE AND MAY
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may
NOT BE REFERRED TO AS AN INTERNATIONAL
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NATIONAL REGULATIONS.
Website: www.iso.org ISO 6887-1:2017/DAM 1:2023(E)
RECIPIENTS OF THIS DRAFT ARE INVITED
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NOTIFICATION OF ANY RELEVANT PATENT
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ii
PROVIDE SUPPORTING DOCUMENTATION. © ISO 2023
SIST EN ISO 6887-1:2017/oprA1:2023
ISO 6887-1:2017/DAM 1:2023(E)
Foreword
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bodies (ISO member bodies). The work of preparing International Standards is normally carried out
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electrotechnical standardization.
The procedures used to develop this document and those intended for its further maintenance are
described in the ISO/IEC Directives, Part 1. In particular the different approval criteria needed for the
different types of ISO documents should be noted. This document was drafted in accordance with the
editorial rules of the ISO/IEC Directives, Part 2 (see www.iso.org/directives).
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expressions related to conformity assessment, as well as information about ISO's adherence to the
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URL: www.iso.org/iso/foreword.html.
This document was prepared by Technical Committee ISO/TC 34, Food products, Subcommittee SC 9,
Microbiology in collaboration with the European Committee for Standardization (CEN) Technical
Committee CEN/TC 463, Microbiology of the food chain, in accordance with the Agreement on technical
cooperation between ISO and CEN (Vienna Agreement).
A list of parts in the ISO 6887 series can be found on the ISO website.
Any feedback or questions on this document should be directed to the user’s national standards body. A
complete listing of these bodies can be found at www.iso.org/members.html.
iii
SIST EN ISO 6887-1:2017/oprA1:2023
SIST EN ISO 6887-1:2017/oprA1:2023
ISO 6887-1:2017/DAM 1:2023(E)
Microbiology of the food chain — Preparation of test
samples, initial suspension and decimal dilutions for
microbiological examination —
Part 1:
General rules for the preparation of the initial suspension
and decimal dilutions
AMENDMENT 1: Requirements and guidance on the use of
larger test portion size for qualitative methods
3.2
Replace the text with the following:
3.2
composite sample
mixed sample of a number of the same items of food, animal feed, animals or environment, prepared in
or out of the laboratory from which a test portion is taken for examination.
Note 1 to entry: See Table A.1 for illustration of a composite sample.
3.3
Replace the text with the following:
3.3
pooled sample
mixed sample of a number of the same items of food, animal feed, animals or environment, prepared
in or out of the laboratory where the complete mixture is the test portion and is taken as a whole for
examination.
Add after 3.10 the following:
3.11
category
group of sample types of the same origin
EXAMPLE Heat-processed milk and dairy products.
[SOURCE: ISO 16140-1:2016]
3.12
type
for a given category, a group of items processed in a similar way, with similar intrinsic characteristics
and a similar microbial ecology
EXAMPLE Food category: heat-processed milk and dairy products; food type: pasteurized dairy product.
SIST EN ISO 6887-1:2017/oprA1:2023
ISO 6887-1:2017/DAM 1:2023(E)
[SOURCE: ISO 16140-1:2016]
3.13
item
single specified food, feed, environmental, or primary production matrix
EXAMPLE Food category: heat-processed milk and dairy products; food type: pasteurized dairy product;
food item: milk-based desserts.
[SOURCE: ISO 16140-1:2016]
3.14
larger test portion
measured (volume or mass) representative sample taken from the laboratory sample (3.1) or test
sample (3.4) for use in the preparation of the initial suspension that is larger than the test portion size
that has been described in the original method and/or validation document
9.3
Replace the text with the following:
9.3 Composite sample and larger test portion
9.3.1 Composite sample
A composite sample is where a number of the same items are mixed, and a test portion is taken for
examination in the laboratory as illustrated in Annex A (Table A.1). The size of the test portion removed
from the composite sample will remain the same as described in the original method and/or validation
document.
Compositing shall be applicable to qualitative tests only.
A number of items may be composited at the sampling stage by the client (out of the laboratory) or by
the laboratory (at client’s request). Only items from the same origin or source (e. g. same batch or lot)
shall be composited.
9.3.2 Larger test portion
A larger test portion is a sample that is larger than the test portion size that has been described in the
original method and/or validation document.
Testing a larger test portion may be necessary 1) to reflect microbiological quality of a large batch
of product, 2) in case when a large number of environmental samples are taken or 3) as sometimes
required by national or regional legislation.
A larger test portion can originate from a single larger test portion (9.3.2.1) or from the pooling of
samples (9.3.2.2).
If a laboratory sample larger than the maximum sample size has been submitted, the sample can be
split into multiple test portions based on the maximum sample size (e.g. if a laboratory sample of 750 g
has been received for Salmonella testing it can be prepared as two 375 g test portions).
9.3.2.1 Larger single test portion
A larger single test portion is a sample that is larger than the test portion size that has been described
in the original method and/or validation document originating from a single sample. A larger single test
portion can be applied to both qualitative and quantitative testing.
9.3.2.2 Pooled sample
SIST EN ISO 6887-1:2017/oprA1:2023
ISO 6887-1:2017/DAM 1:2023(E)
A pooled sample is a sample where a number of items have been combined and the complete mixture is
taken as a whole for examination in the laboratory. Items can be pooled either:
a. out of the laboratory, where individual samples are combined into one larger pooled sample
b. or in the laboratory where individual test portions are combined into one larger (pre-) enrichment
as illustrated in Annex A (Table A.1).
c. or in the laboratory , where individual (pre-) enriched test portions items are combined into one
and carried through as a single test as illustrated in Annex A (Table A.1)
A number of items may be pooled at the sampling stage by the client or laboratory (at client’s request).
Pooling shall be applicable to qualitative tests only. Only items from the same origin or source (e. g.
same batch or lot) shall be pooled.
9.3.3. Procedure for larger test portion size
Items can either be composited, pooled out of the laboratory or in the laboratory as test portion or as
(pre) enriched test portion but not as two or more combinations (i.e., pooling of (pre) enriched as well
as test portion is not allowed).
To minimize the risk of false negative results when testing a larger single test portion or pooled test
portion.
— The primary diluent shall be pre warmed to the intended incubation temperature with the same
tolerance range.
— The temperature profile of the larger enrichment volume should be checked to ensure that the time
taken to reach the target incubation temperature and overall incubation time are as specified in
the individual standard. For example, in ISO 6579-1:2017 the pre-enrichment incubation time is
between 16 h and 20 h. For larger test portion size the pre-enrichment incubation time shall be
between 34 °C and 38 °C for a minimum of 16 h.
— When testing a larger single test portion, pooled test portion and pooled (pre-) enrichment test
portion, the dilution ratio (sample/diluent) used in the validated method shall remain the same.
This ratio may be increased to overcome the inhibitory effects coming from the sample (examples
are described in ISO 6887-4:2017, 9.1.4.4).
Additional, pooling instructions may be described in individual standards (e.g. maximum sample size
and food category).
9.3.4 Validation and verifi
...
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