ISO 10727:2002

INTERNATIONAL ISO
STANDARD 10727
Second edition
2002-07-15
Tea and instant tea in solid form —
Determination of caffeine content — Method
using high-performance liquid
chromatography
Thé et thé soluble sous forme solide — Détermination de la teneur en
caféine — Méthode par chromatographie liquide à haute performance

Reference number
ISO 10727:2002(E)
© ISO 2002

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ISO 10727:2002(E)
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ISO 10727:2002(E)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards bodies (ISO
member bodies). The work of preparing International Standards is normally carried out through ISO technical
committees. Each member body interested in a subject for which a technical committee has been established has
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Commission (IEC) on all matters of electrotechnical standardization.
International Standards are drafted in accordance with the rules given in the ISO/IEC Directives, Part 3.
Draft International Standards adopted by the technical committees are circulated to the member bodies for voting.
Publication as an International Standard requires approval by at least 75 % of the member bodies casting a vote.
Attention is drawn to the possibility that some of the elements of this International Standard may be the subject of
patent rights. ISO shall not be held responsible for identifying any or all such patent rights.
International Standard ISO 10727 was prepared by Technical Committee ISO/TC 34, Food products, Subcommittee
SC 8, Tea.
This second edition cancels and replaces the first edition (ISO 10727:1995), which has been technically revised.
Annex A of this International Standard is for information only.
©
ISO 2002 – All rights reserved iii

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INTERNATIONAL STANDARD ISO 10727:2002(E)
Tea and instant tea in solid form — Determination of caffeine
content — Method using high-performance liquid chromatography
1 Scope
This International Standard specifies a method for the determination by high-performance liquid chromatography
(HPLC) of the caffeine content of teas and instant teas. It is applicable to green tea, black tea and decaffeinated tea
products.
2 Normative references
The following normative documents contain provisions which, through reference in this text, constitute provisions of
this International Standard. For dated references, subsequent amendments to, or revisions of, any of these
publications do not apply. However, parties to agreements based on this International Standard are encouraged to
investigate the possibility of applying the most recent editions of the normative documents indicated below. For
undated references, the latest edition of the normative document referred to applies. Members of ISO and IEC
maintain registers of currently valid International Standards.
ISO 1572:1980, Tea — Preparation of ground sample of known dry matter content
ISO 3696:1987, Water for analytical laboratory use — Specification and test methods
ISO 7513:1990, Instant tea in solid form — Determination of moisture content (loss in mass at 103 °C)
3 Principle
The caffeine from a test portion is extracted by reflux with water in the presence of magnesium oxide. After filtration,
...