ISO 11050:2020

SLOVENSKI STANDARD
01-december-2020
Nadomešča:
SIST ISO 11050:1997
Pšenična moka in pšenični zdrob durum - Določevanje nečistoč živalskega izvora
Wheat flour and durum wheat semolina -- Determination of impurities of animal origin
Farines de blé tendre et semoules de blé dur -- Détermination des impuretés d'origine
animale
Ta slovenski standard je istoveten z: ISO 11050:2020
ICS:
67.060 Žita, stročnice in proizvodi iz Cereals, pulses and derived
njih products
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

INTERNATIONAL ISO
STANDARD 11050
Second edition
2020-04
Wheat flour and durum wheat
semolina — Determination of
impurities of animal origin
Farines de blé tendre et semoules de blé dur — Détermination des
impuretés d'origine animale
Reference number
©
ISO 2020
© ISO 2020
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ii © ISO 2020 – All rights reserved

Contents Page
Foreword .iv
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Principle . 3
5 Reagents . 3
6 Apparatus . 3
7 Sampling . 5
8 Procedure. 6
8.1 Test portion . 6
8.2 Hydrolysis . 6
8.3 Separation of impurities . 6
8.4 Filtration . 7
8.5 Microscopic examination . 7
8.6 Number of determinations . 8
9 Expression of results . 8
10 Repeatability . 8
11 Test report . 8
Annex A (informative) Characteristics of fragments found on the filters . 9
Annex B (informative) Test report — Determination of animal impurities in accordance
with this document .11
Annex C (informative) Diagram of the procedure .12
Annex D (informative) Chronology of operations and timetable .14
Bibliography .15
Foreword
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different types of ISO documents should be noted. This document was drafted in accordance with the
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iso/ foreword .html.
This document was prepared by Technical Committee ISO/TC 34, Food products, Subcommittee SC 4,
Cereals and pulses.
This second edition cancels and replaces the first edition (ISO 11050:1993), which has been technically
revised. The main changes compared with the previous edition are as follows:
— the Scope has been widened;
— the protocol has been improved to make it easier;
— the figures have been updated.
Any feedback or questions on this document should be directed to the user’s national standards body. A
complete listing of these bodies can be found at www .iso .org/ members .html.
iv © ISO 2020 – All rights reserved

INTERNATIONAL STANDARD ISO 11050:2020(E)
Wheat flour and durum wheat semolina — Determination
of impurities of animal origin
1 Scope
This document specifies a method for determining the content of impurities of animal origin in wheat
flours, with or without additives and having an ash yield not exceeding a mass fraction of 0,75 %, and in
durum wheat semolinas.
This method permits the separation and quantification of contamination of animal origin, such as insects
at all stages of their development and their fragments, rodent hairs and their fragments, and mites.
2 Normative references
There are no normative references in this document.
3 Terms and definitions
For the purposes of this document, the following terms and definitions apply.
ISO and IEC maintain terminological databases for use in standardization at the following addresses:
— ISO Online browsing platform: available at https:// www .iso .org/ obp
— IEC Electropedia: available at http:// www .electropedia .org/
3.1
impurity of animal origin
matter of animal origin [larvae, nymphs or adults of insects (3.10) and their fragments, rodent hairs
and their fragments, mites (3.3)] separated from the product under the conditions specified in this
document
3.2
abdomen
rear part of the body of an insect (3.10), excluding the head and thorax, commonly with eight or more
segments when complete
3.3
mite
very small arthropod belonging to the class Arachnida, often living in large numbers
3.4
appendice
distinctly differentiated prolongation of the body of an arthropod
EXAMPLE Legs, wings, antennae, urogomphi (3.16).
3.5
cephalic capsule
head capsule
sclerous part of an exuvia that once contained the head of a larva
3.6
caterpillar
larvae of Lepidoptera spp.
Note 1 to entry: Butterfly or moth is the adult stage and chrysalis is the pupal stage.
3.7
scale
bristle (3.13) that has evolved into a flat structure resembling a fish scale and that covers the parts of
the body of certain insects (3.10), in particular the wings of Lepidoptera spp.
3.8
wing case
elytron
hardened front wing of Coleoptera spp.
Note 1 to entry: It is used as a fixed wing in flight and as protective cover for the membranous hind wing.
3.9
false leg
proleg
fleshy extension of the lower part of the abdomen (3.2) of some larvae, sometimes with a crown of fine
hooks (crochets) of chitin
Note 1 to entry: These help with attachment to the substrate, and in movement. Lepidopterous larvae have at
least two pairs of false legs, towards the rear of the body.
3.10
insect
class of animals within the phylum Arthropoda, some of which are recognized pests of stored foodstuffs
3.11
mandible
toughened (sclerotized) mouthpart of insects (3.10)
Note 1 to entry: It is used for the dilaceration or grinding of food.
3.12
pericarp
external envelope of seeds that forms the bran after the grain has been crushed and the flour separated
3.13
bristle
fine but stiff hair of any length present on the cuticle of insects (3.10)
Note 1 to entry: Sensory hairs are called “setae”.
3.14
stage
state of development of an insect (3.10) or mite (3.3)
EXAMPLE Egg, larva, nymph, pupa, adult.
3.15
juvenile stage
pre-adult stage (3.14) of insects (3.10)
EXAMPLE Egg, larva, nymph, pupa.
Note 1 to entry: This term is most often applied to the active stages of larva and nymph.
2 © ISO 2020 – All rights reserved

3.16
urogomphi
pointed extensions of the cuticle of the final abdominal segment of some insect larvae
Note 1 to entry: They are common, and sometimes diagnostic, features of many Coleoptera spp.
Note 2 to entry: The abdominal extensions of a cockroach are called “cerci”.
4 Principle
Hydrolysis of a test portion with a solution of hydrochloric acid at boiling point. Concentration of the
insoluble particles (impurities other than those of animal origin may be present) at a water/oil interface.
Separation by filtration on a filter paper, microscopic examination and counting under reflected light of
the impurities of animal origin.
5 Reagents
Only use reagents of a recognized analytical grade and demineralized water or water of equivalent purity.
5.1 Ethanol, a volume fraction of 95 %.
5.2 Ethanol solution, a volume fraction of 50 %.
5.3 Ethanol/glycerol, 1 + 1 mixture by volume.
5.4 Hydrochloric acid solution, concentrated at 35 % to 37 %.
5.5 Paraffin oil (known as “Vaseline oil”), fluid, having a viscosity not exceeding 60 mPa·s at 20 °C.
5.6 Liquid detergent, non-foaming.
1)
EXAMPLE Extran, Biodeck 4, Decon 90.
5.7 Liquid detergent, a volume fraction of 1 % aqueous solution of the detergent (5.6) in a
washing bottle.
5.8 Dearomatized white spirit (possible use).
6 Apparatus
Usual laboratory apparatus and, in particular, the following.
1) These are examples of suitable products available commercially. This information is given for the convenience
of users of this document and does not constitute an endorsement by ISO of these products.
6.1 Separating funnels, conical, of 1 000 ml capacity, fitted with a non-lubricat
...