EN ISO 14565:2000
(Main)Animal feeding stuffs - Determination of vitamin A content - Method using high-performance liquid chromatography (ISO 14565:2000)
Animal feeding stuffs - Determination of vitamin A content - Method using high-performance liquid chromatography (ISO 14565:2000)
Futtermittel - Bestimmung des Gehalts an Vitamin A - Verfahren mit Hochleistungs-Flüssigchromatographie (ISO 14565:2000)
Diese Internationale Norm legt ein Verfahren zur Bestimmung des Gesamtgehalts an Vitamin-A (Retinol) in Futtermitteln und in Heimtierfuttermitteln mittels Hochleistungs-Flüssigkeitschromatographie (HPLC) fest.
Aliments des animaux - Détermination de la teneur en vitamine A - Méthode par chromatographie liquide à haute performance (ISO 14565:2000)
La présente Norme internationale spécifie une méthode de détermination de la teneur en vitamine A totale (rétinol) des aliments pour animaux d'élevage et animaux de compagnie par chromatographie liquide à haute performance. La teneur en vitamine A est la teneur en alcool de rétinyle tout-trans et cis-isomères déterminée par la méthode décrite dans la présente Norme internationale, exprimée en Unités internationales par kilogramme (Ul/kg).
Krma - Določevanje vitamina A - Metoda z uporabo tekočinske kromatografije visoke ločljivosti (ISO 14565:2000)
General Information
- Status
- Published
- Publication Date
- 30-Nov-2000
- Technical Committee
- CEN/TC 327 - Animal feeding stuffs - Methods of sampling and analysis
- Drafting Committee
- CEN/TC 327 - Animal feeding stuffs - Methods of sampling and analysis
- Current Stage
- 9093 - Decision to confirm - Review Enquiry
- Start Date
- 16-May-2007
- Completion Date
- 16-May-2007
Relations
- Effective Date
- 09-Feb-2026
- Consolidated By
ISO 14565:2000 - Animal feeding stuffs — Determination of vitamin A content — Method using high-performance liquid chromatography - Effective Date
- 12-Feb-2026
Overview
EN ISO 14565:2000 - Animal feeding stuffs - Determination of vitamin A content - Method using high-performance liquid chromatography (ISO 14565:2000) - defines a laboratory method to determine the concentration of vitamin A in animal feeds using high-performance liquid chromatography (HPLC). Adopted by CEN and mirrored in national publications (e.g., SIST EN ISO 14565:2001), this standard provides a harmonized analytical approach for feed analysis and quality control.
Key Topics
The standard addresses the main elements needed to perform accurate, reproducible vitamin A determinations in feed samples:
- Analytical technique: Use of high-performance liquid chromatography (HPLC) for separation and quantification of vitamin A compounds.
- Sample handling: Requirements for representative sampling, preparation and extraction to ensure reliable results.
- Calibration and quantification: Use of appropriate reference materials or standards and procedures for generating calibration curves and calculating results.
- Method validation and quality control: Criteria for precision, accuracy, limits of detection/quantification, and routine QC checks to maintain analytical performance.
- Reporting: Recommended ways to express results (units, reporting limits) and information to include in test reports for traceability.
(Note: the published extract does not include detailed step-by-step parameters; laboratories should consult the full EN ISO 14565:2000 document for exact procedural details.)
Applications
This standard is practical and widely used for:
- Feed manufacturers performing in-house quality control to confirm vitamin A levels in premixes, compounded feeds and finished products.
- Analytical testing laboratories offering accredited feed analysis services based on standardized HPLC methods.
- Regulatory bodies and inspectors verifying compliance with nutritional labeling and statutory vitamin fortification limits.
- Animal nutritionists and formulators ensuring correct dosing of vitamin A for animal health, growth performance and product quality.
- Research groups studying vitamin stability in feed matrices, processing effects, or bioavailability.
Using EN ISO 14565:2000 helps ensure consistent, comparable vitamin A results across laboratories and supports product safety, compliance and formulation accuracy.
Related Standards
- Other ISO/CEN methods for feed analysis and nutrient determination (e.g., methods for vitamins, minerals and proximate composition) are commonly used alongside EN ISO 14565:2000 to provide a complete nutritional profile.
- Laboratories should align this method with their national accreditation requirements and any regulatory guidance relevant to feed composition and labeling.
Keywords: EN ISO 14565:2000, vitamin A determination, animal feeding stuffs, high-performance liquid chromatography, HPLC, feed analysis, vitamin A content.
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Frequently Asked Questions
EN ISO 14565:2000 is a standard published by the European Committee for Standardization (CEN). Its full title is "Animal feeding stuffs - Determination of vitamin A content - Method using high-performance liquid chromatography (ISO 14565:2000)". This standard covers: La présente Norme internationale spécifie une méthode de détermination de la teneur en vitamine A totale (rétinol) des aliments pour animaux d'élevage et animaux de compagnie par chromatographie liquide à haute performance. La teneur en vitamine A est la teneur en alcool de rétinyle tout-trans et cis-isomères déterminée par la méthode décrite dans la présente Norme internationale, exprimée en Unités internationales par kilogramme (Ul/kg).
La présente Norme internationale spécifie une méthode de détermination de la teneur en vitamine A totale (rétinol) des aliments pour animaux d'élevage et animaux de compagnie par chromatographie liquide à haute performance. La teneur en vitamine A est la teneur en alcool de rétinyle tout-trans et cis-isomères déterminée par la méthode décrite dans la présente Norme internationale, exprimée en Unités internationales par kilogramme (Ul/kg).
EN ISO 14565:2000 is classified under the following ICS (International Classification for Standards) categories: 65.120 - Animal feeding stuffs. The ICS classification helps identify the subject area and facilitates finding related standards.
EN ISO 14565:2000 has the following relationships with other standards: It is inter standard links to ISO 3696:1987, ISO 14565:2000. Understanding these relationships helps ensure you are using the most current and applicable version of the standard.
EN ISO 14565:2000 is available in PDF format for immediate download after purchase. The document can be added to your cart and obtained through the secure checkout process. Digital delivery ensures instant access to the complete standard document.
Standards Content (Sample)
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.WRJUDILMHFuttermittel - Bestimmung des Gehalts an Vitamin A - Verfahren mit Hochleistungs-Flüssigchromatographie (ISO 14565:2000)Aliments des animaux - Détermination de la teneur en vitamine A - Méthode par chromatographie liquide a haute performance (ISO 14565:2000)Animal feeding stuffs - Determination of vitamin A content - Method using high-performance liquid chromatography (ISO 14565:2000)65.120KrmilaAnimal feeding stuffsICS:Ta slovenski standard je istoveten z:EN ISO 14565:2000
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