EN 15204:2006
(Main)Water quality - Guidance standard on the enumeration of phytoplankton using inverted microscopy (Utermöhl technique)
Water quality - Guidance standard on the enumeration of phytoplankton using inverted microscopy (Utermöhl technique)
The procedure described in this European Standard is based on the standard settling technique as defined by Utermöhl in 1958 [31]. It describes a general procedure for the estimation of abundance and taxonomic composition of marine and freshwater phytoplankton by using inverted light microscopy and sedimentation chambers, including the preceding steps of preservation and storage. Emphasis is placed on optimizing the procedure for the preparation of the microscopic sample. Many of the general principles of the approach described may also be applied to other techniques of enumerating algae (or other entities) using a (conventional) microscope, some of which are described in Annex E. This guidance standard does not cover field collection of samples or the analysis of picoplankton, quantitative analysis of free-floating mats of Cyanobacteria or specific preparation techniques for diatoms.
Wasserbeschaffenheit - Anleitung für die Zählung von Phytoplankton mittels der Umkehrmikroskopie (Utermöhl-Technik)
Diese Europäischen Norm legt ein Verfahren für das Standard-Sedimentationsverfahren fest, das auf dem von Utermöhl 1958 beschriebenen Test [31] beruht. Es beschreibt ein allgemeines Verfahren für die Abschätzung der Abundanz (Häufigkeit) und taxonomischen Zusammensetzung des marinen und limnischen Phytoplanktons unter Verwendung der Durchlichtumkehrmikroskopie und von Sedimentationskammern, einschließlich der vorausgehenden Schritte der Konservierung und Lagerung der Proben. Der Schwerpunkt liegt auf der Optimierung des Verfahrens für die Vorbereitung der zu mikroskopierenden Probe. Viele der allgemeinen Prinzipien können auch auf andere Techniken der Algenzählung (oder anderer Organismen) mit einem (konventionellen) Mikroskop angewandt werden; einige sind im Anhang E beschrieben. Diese Anleitung behandelt weder die Feldprobenahme noch die Analyse des Picoplanktons, noch die quantitative Analyse frei treibender Matten von Cyanobakterien (Blaualgen) noch spezifische Präparationstechniken für Kieselalgen.
Qualité de l'eau - Norme guide pour le dénombrement du phytoplancton par microscopie inversée (méthode Utermöhl)
Le mode opératoire décrit dans la présente Norme européenne est fondé sur la technique de sédimentation classique telle que définie par Utermöhl en 1958 [31]. Il s’agit d’un mode opératoire général pour l’estimation de l’abondance et de la composition taxinomique du phytoplancton marin et d’eau douce à l’aide d’un microscope inversé et de chambres de sédimentation, y compris les étapes préalables de conservation et de stockage. L’accent est mis sur l’optimisation du mode opératoire pour la préparation de l’échantillon microscopique. Un grand nombre de principes généraux de l’approche décrite, dont certains sont mentionnés dans l’Annexe E, peuvent aussi s’appliquer à d’autres techniques de dénombrement d’algues (ou d’autres entités) à l’aide d’un microscope (classique). La présente norme guide ne concerne pas la collecte d’échantillons sur le terrain, l’analyse du picoplancton, l’analyse quantitative d’amas flottants de cyanobactéries, ou les techniques spécifiques pour la préparation des diatomées.
Kakovost vode - Smerni standard za štetje fitoplanktona z invertno mikroskopijo (postopek po Utermöhlu)
General Information
Standards Content (Sample)
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EUROPEAN STANDARDNORME EUROPÉENNEEUROPÄISCHE NORMEN 15204August 2006ICS 13.060.70 English VersionWater quality - Guidance standard on the enumeration ofphytoplankton using inverted microscopy (Utermöhl technique)Qualité de l'eau - Norme guide pour l'analyse de routine del'abondance et de la composition du phytoplancton parmicroscopie inversée (méthode d'Utermöhl)Wasserbeschaffenheit - Anleitung für die Zählung vonPhytoplankton mittels der Umkehrmikroskopie (Utermöhl-Technik)This European Standard was approved by CEN on 14 July 2006.CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this EuropeanStandard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such nationalstandards may be obtained on application to the Central Secretariat or to any CEN member.This European Standard exists in three official versions (English, French, German). A version in any other language made by translationunder the responsibility of a CEN member into its own language and notified to the Central Secretariat has the same status as the officialversions.CEN members are the national standards bodies of Austria, Belgium, Cyprus, Czech Republic, Denmark, Estonia, Finland, France,Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania,Slovakia, Slovenia, Spain, Sweden, Switzerland and United Kingdom.EUROPEAN COMMITTEE FOR STANDARDIZATIONCOMITÉ EUROPÉEN DE NORMALISATIONEUROPÄISCHES KOMITEE FÜR NORMUNGManagement Centre: rue de Stassart, 36
B-1050 Brussels© 2006 CENAll rights of exploitation in any form and by any means reservedworldwide for CEN national Members.Ref. No. EN 15204:2006: E
Optical characteristics of inverted microscopes.21 Annex B (informative)
Sample treatment.23 Annex C (informative)
Phytoplankton analysis strategies.27 Annex D (informative)
Identification.30 Annex E (informative)
Use of conventional compound microscopes.31 Annex F (informative)
Statistical procedure.34 Bibliography.40
Figures Figure 1 — Random distribution of particles (note the open spaces).12 Figure 2 — Example of rule for counting cells on the edge of the field. Algae objects crossing both the top and left hand side of grid are not counted whilst those crossing the bottom and right hand side of grid are counted.14 Figure F.1 — Illustration of collecting algal data for a Run-test.35
Tables Table 1 — Settling times for Lugol preserved seawater samples [12].11 Table F.F1 — Maximum allowable variance for Poisson approximation (µ = mean, σσσσ 2 = variance).35 Table F.F2 — Multinomial homogeneity test.36
3.13 preservation process that protects organic substances from decay 3.14 (analysis) protocol specific analytical procedure concerning (sub)sample volume, magnification, number of cells to count, taxonomic level of identification etc. 3.15 repeatability precision under repeatability conditions 3.16 repeatability conditions conditions where independent test/measurement results are obtained with the same method on identical test/measurement items in the same test or measuring facility by the same operator using the same equipment within short intervals of time NOTE This definition should be interpreted as the error occurring between replicate sub-samples from the same sample, counted using the same counting chamber, performed by one analyst using one microscope in a continuous run on one day. 3.17 reproducibility precision under reproducibility conditions
3.18 reproducibility conditions conditions where independent test/measurement results are obtained with the same method on identical test/measurement items in different test or measurement facilities with different operators using different equipment
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