EN ISO 24199:2022

SLOVENSKI STANDARD
01-september-2022
Hlapni proizvodi - Ugotavljanje deleža nikotina v emisijah hlapnih proizvodov -
Metoda plinske kromatografije (ISO 24199:2022)
Vapour products - Determination of nicotine in vapour product emissions - Gas
chromatographic method (ISO 24199:2022)
Dampfprodukte - Bestimmung von Nikotin in Emissionen von Dampfprodukten -
Gaschromatographisches Verfahren (ISO 24199:2022)
Produits de vapotage - Détermination de la teneur en nicotine dans les émissions de
produits de vapotage - Méthode par chromatographie en phase gazeuse (ISO
24199:2022)
Ta slovenski standard je istoveten z: EN ISO 24199:2022
ICS:
65.160 Tobak, tobačni izdelki in Tobacco, tobacco products
oprema and related equipment
71.040.50 Fizikalnokemijske analitske Physicochemical methods of
metode analysis
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

EN ISO 24199
EUROPEAN STANDARD
NORME EUROPÉENNE
July 2022
EUROPÄISCHE NORM
ICS 65.160
English Version
Vapour products - Determination of nicotine in vapour
product emissions - Gas chromatographic method (ISO
24199:2022)
Produits de vapotage - Détermination de la teneur en Dampfprodukte - Bestimmung von Nikotin in
nicotine dans les émissions de produits de vapotage - Emissionen von Dampfprodukten -
Méthode par chromatographie en phase gazeuse (ISO Gaschromatographisches Verfahren (ISO 24199:2022)
24199:2022)
This European Standard was approved by CEN on 2 July 2022.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this
European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by
translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management
Centre has the same status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Türkiye and
United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2022 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 24199:2022 E
worldwide for CEN national Members.

Contents Page
European foreword . 3

European foreword
This document (EN ISO 24199:2022) has been prepared by Technical Committee ISO/TC 126 "Tobacco
and tobacco products" in collaboration with Technical Committee CEN/TC 437 “Electronic cigarettes
and e-liquids” the secretariat of which is held by AFNOR.
This European Standard shall be given the status of a national standard, either by publication of an
identical text or by endorsement, at the latest by January 2023, and conflicting national standards shall
be withdrawn at the latest by January 2023.
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. CEN shall not be held responsible for identifying any or all such patent rights.
Any feedback and questions on this document should be directed to the users’ national standards
body/national committee. A complete listing of these bodies can be found on the CEN website.
According to the CEN-CENELEC Internal Regulations, the national standards organizations of the
following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria,
Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland,
Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Republic of
North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Türkiye and the
United Kingdom.
Endorsement notice
The text of ISO 24199:2022 has been approved by CEN as EN ISO 24199:2022 without any modification.

INTERNATIONAL ISO
STANDARD 24199
First edition
2022-07
Vapour products — Determination of
nicotine in vapour product emissions
— Gas chromatographic method
Produits de vapotage — Détermination de la teneur en nicotine
dans les émissions de produits de vapotage — Méthode par
chromatographie en phase gazeuse
Reference number
ISO 24199:2022(E)
ISO 24199:2022(E)
© ISO 2022
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may
be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting on
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or ISO’s member body in the country of the requester.
ISO copyright office
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Phone: +41 22 749 01 11
Email: copyright@iso.org
Website: www.iso.org
Published in Switzerland
ii
ISO 24199:2022(E)
Contents Page
Foreword .iv
Introduction .v
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Principle . 2
5 Reagents . 2
6 Apparatus . 3
7 Procedure .3
7.1 Preparation of test samples . 3
7.2 Glass fibre filter pads handling . 3
7.3 Aerosol collection and sample preparation . 3
7.4 Test portion . 4
7.5 Setting up the apparatus . 4
7.6 Calibration of the gas chromatograph . 5
7.7 Determination . 5
8 Expression of results . 5
9 Repeatability and reproducibility .6
9.1 General . 6
9.2 Results of an interlaboratory study (Study 1) . 6
9.3 Results of an interlaboratory study (Study 2) . 6
10 Test report . 7
Annex A (informative) Example chromatogram for nicotine analysis . 8
Bibliography . 9
iii
ISO 24199:2022(E)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards
bodies (ISO member bodies). The work of preparing International Standards is normally carried out
through ISO technical committees. Each member body interested in a subject for which a technical
committee has been established has the right to be represented on that committee. International
organizations, governmental and non-governmental, in liaison with ISO, also take part in the work.
ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of
electrotechnical standardization.
The procedures used to develop this document and those intended for its further maintenance are
described in the ISO/IEC Directives, Part 1. In particular, the different approval criteria needed for the
different types of ISO documents should be noted. This document was drafted in accordance with the
editorial rules of the ISO/IEC Directives, Part 2 (see www.iso.org/directives).
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. ISO shall not be held responsible for identifying any or all such patent rights. Details of
any patent rights identified during the development of the document will be in the Introduction and/or
on the ISO list of patent declarations received (see www.iso.org/patents).
Any trade name used in this document is information given for the convenience of users and does not
constitute an endorsement.
For an explanation of the voluntary nature of standards, the meaning of ISO specific terms and
expressions related to conformity assessment, as well as information about ISO's adherence to
the World Trade Organization (WTO) principles in the Technical Barriers to Trade (TBT), see
www.iso.org/iso/foreword.html.
This document was prepared by Technical Committee ISO/TC 126, Tobacco and tobacco products,
Subcommittee SC 3, Vape and vapour products, in collaboration with the European Committee for
Standardization (CEN) Technical Committee CEN/TC 437, Electronic cigarettes and e-liquids, in
accordance with the Agreement on technical cooperation between ISO and CEN (Vienna Agreement).
Any feedback or questions on this document should be directed to the user’s national standards body. A
complete listing of these bodies can be found at www.iso.org/members.html.
iv
ISO 24199:2022(E)
Introduction
In many countries, the regulation of vapour products requires reporting for nicotine compounds in
emissions. Therefore, there is a necessity to have an International Standard in place to get reliable/
comparable data on nicotine in electronic cigarette emissions.
This document was developed for the determination of nicotine in the aerosol from vapour products
utilizing gas chromatography coupled with a flame ionization detector. The experimental design
[1][2]
parameters used to collect the aerosolised vapour should be evaluated and documented for each
analysis.
[3]
The document is based on the CORESTA recommended method (CRM) 84 , which was written on the
[4]
basis of the results obtained in an interlaboratory study conducted in 2015 involving 18 laboratories
[5]
and an interlaboratory study conducted in 2019 invol
...