EN 16075:2011
(Main)Fertilizers - Determination of N-(2-nitrophenyl)phosphoric triamide (2-NPT) in urea and fertilizers containing urea - Method using high-performance liquid chromatography (HPLC)
Fertilizers - Determination of N-(2-nitrophenyl)phosphoric triamide (2-NPT) in urea and fertilizers containing urea - Method using high-performance liquid chromatography (HPLC)
This European Standard specifies a method for the determination of N-(2-nitrophenyl)phosphoric triamide (2-NPT) in urea or in fertilizers containing urea using high-performance liquid chromatography (HPLC).
Düngemittel - Bestimmung von N-(2-Nitrophenyl)Phosphorsäure-Triamid (2-NPT) in Harnstoff und harnstoffhaltigen Düngemitteln - Verfahren mit Hochleistungs-Flüssigchromatographie (HPLC)
Diese Europäische Norm legt ein Verfahren zur Bestimmung von N-(2-Nitrophenyl)Phosphorsäure-Triamid (2-NPT) in Harnstoff oder harnstoffhaltigen Düngemitteln mit Hochleistungs-Flüssigchromatographie (HPLC) fest.
Engrais - Dosage du N-(2-nitrophényl) triamide phosphorique (2-NPT) dans l'urée et les engrais contenant de l'urée - Méthode par chromatographie liquide à haute performance (HPLC)
La présente norme européenne décrit une méthode de dosage du N-(2-nitrophényl) triamide phosphorique (2 NPT) dans l’urée et les engrais contenant de l’urée, par chromatographie liquide à haute performance (HPLC).
Gnojila - Določevanje N-(2-nitrofenil) triamida fosforne kisline (2-NPT) v sečnini in v gnojilih, ki vsebujejo sečnino - Metoda s tekočinsko kromatografijo visoke ločljivosti (HPLC)
Ta dokument določa metodo za določevanje N–(2–nitrofenil) triamida fosforne kisline (2–NPT) s tekočinsko kromatografijo visoke ločljivosti (HPLC) v sečnini ali gnojilih, ki vsebujejo sečnino.
General Information
- Status
- Published
- Publication Date
- 01-Nov-2011
- Withdrawal Date
- 30-May-2012
- Technical Committee
- CEN/TC 260 - Fertilizers and liming materials
- Drafting Committee
- CEN/TC 260/WG 7 - Chemical analysis
- Current Stage
- 9093 - Decision to confirm - Review Enquiry
- Start Date
- 20-Jun-2023
- Completion Date
- 14-Apr-2025
Overview
EN 16075:2011 is a European Standard developed by CEN (European Committee for Standardization) that specifies a precise analytical method for determining the concentration of N-(2-nitrophenyl)phosphoric triamide (2-NPT) in urea and fertilizers containing urea. The method utilizes high-performance liquid chromatography (HPLC) with UV detection, ensuring accurate, reliable, and reproducible results for quality control and regulatory compliance in fertilizer production and testing.
This standardized approach is crucial for fertilizer manufacturers, laboratories, and regulatory bodies needing to measure 2-NPT-a chemical compound used in fertilizers-to ensure product safety and effectiveness. The standard harmonizes testing procedures across Europe, facilitating mutual recognition of test results and aiding in compliance with EU fertilizer regulations.
Key Topics
Scope and Purpose
Defines the application of HPLC for qualitative and quantitative determination of 2-NPT in urea-based fertilizers.Sampling and Sample Preparation
Outlines sample dissolution and preparation protocols, including special handling when paraffins are present as conditioning agents.Reagents and Apparatus
Requires high-purity reagents like methanol, n-hexane, and water compliant with EN ISO 3696, plus equipment such as analytical balances, ultrasonic baths, syringe filters, and HPLC systems with UV detectors.HPLC Analytical Procedure
Details reversed-phase chromatography conditions:- Mobile phase: water/methanol 50:50
- Flow rate: 0.5 ml/min
- Injection volume: 20 µl
- Detection wavelength: 214 nm
Calibration and Quantification
Describes preparation of calibration standards from 2-NPT, method for constructing calibration curves, and calculation formulas for determining 2-NPT content with precision.Precision and Validation
Includes results from inter-laboratory studies demonstrating repeatability and reproducibility, essential for verifying method robustness and accuracy.Reporting Requirements
Specifies mandatory information to include in test reports, ensuring transparency and traceability.
Applications
Quality Control in Fertilizer Manufacturing
Enables producers to verify 2-NPT levels in urea and urea-based fertilizers, maintaining product consistency and compliance.Regulatory Compliance
Supports laboratories in testing fertilizers in line with European fertilizer regulations and standards, facilitating market access throughout the EU.Research and Development
Assists in formulation optimization and product testing where 2-NPT is involved.Environmental and Safety Testing
Provides data on potential chemical residues, helping monitor environmental impact and occupational health standards.
Related Standards
EN 1482-1 & EN 1482-2
Guidelines on sampling and sample preparation for fertilizers, supporting accurate and representative analysis.EN 12944-1 & EN 12944-2
Fertilizer and soil improver vocabulary standards, ensuring consistent terminology.EN ISO 3696
Standards specifying the quality of water used for analytical laboratory purposes to guarantee test accuracy.ISO 5725-1 & ISO 5725-2
Standards covering accuracy, repeatability, and reproducibility of measurement methods.Regulation (EC) No 2003/2003
Provides the regulatory framework for fertilizer products within the European Union.
Keywords: EN 16075, 2-NPT determination, urea fertilizers, high-performance liquid chromatography, HPLC method, fertilizer analysis, European Standard, fertilizer quality control, CEN standards, analytical method for fertilizers, fertilizer testing, regulatory compliance, pesticide residue analysis, precision agriculture testing.
Frequently Asked Questions
EN 16075:2011 is a standard published by the European Committee for Standardization (CEN). Its full title is "Fertilizers - Determination of N-(2-nitrophenyl)phosphoric triamide (2-NPT) in urea and fertilizers containing urea - Method using high-performance liquid chromatography (HPLC)". This standard covers: This European Standard specifies a method for the determination of N-(2-nitrophenyl)phosphoric triamide (2-NPT) in urea or in fertilizers containing urea using high-performance liquid chromatography (HPLC).
This European Standard specifies a method for the determination of N-(2-nitrophenyl)phosphoric triamide (2-NPT) in urea or in fertilizers containing urea using high-performance liquid chromatography (HPLC).
EN 16075:2011 is classified under the following ICS (International Classification for Standards) categories: 65.080 - Fertilizers. The ICS classification helps identify the subject area and facilitates finding related standards.
EN 16075:2011 is associated with the following European legislation: EU Directives/Regulations: 2003/2003, 2019/1009; Standardization Mandates: M/418. When a standard is cited in the Official Journal of the European Union, products manufactured in conformity with it benefit from a presumption of conformity with the essential requirements of the corresponding EU directive or regulation.
You can purchase EN 16075:2011 directly from iTeh Standards. The document is available in PDF format and is delivered instantly after payment. Add the standard to your cart and complete the secure checkout process. iTeh Standards is an authorized distributor of CEN standards.
Standards Content (Sample)
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.Düngemittel - Bestimmung von N-(2-Nitrophenyl)Phosphorsäure-Triamid (2-NPT) in Harnstoff und harnstoffhaltigen Düngemitteln - Verfahren mit Hochleistungs-Flüssigchromatographie (HPLC)Engrais - Dosage du N-(2-nitrophényl) triamide d'acide phosphorique (2-NPT) dans l'urée et les engrais contenant de l'urée - Méthode par chromatographie liquide à haute performance (HPLC)Fertilizers - Determination of N-(2-nitrophenyl)phosphoric acid triamide (2-NPT) in urea and fertilizers containing urea - Method using high-performance liquid chromatography (HPLC)65.080GnojilaFertilizersICS:Ta slovenski standard je istoveten z:EN 16075:2011SIST EN 16075:2012en,fr,de01-marec-2012SIST EN 16075:2012SLOVENSKI
STANDARD
EUROPEAN STANDARD NORME EUROPÉENNE EUROPÄISCHE NORM
EN 16075
November 2011 ICS 65.080 English Version
Fertilizers - Determination of N-(2-nitrophenyl)phosphoric triamide (2-NPT) in urea and fertilizers containing urea - Method using high-performance liquid chromatography (HPLC)
Engrais - Dosage du N-(2-nitrophényl) triamide phosphorique (2-NPT) dans l'urée et les engrais contenant de l'urée - Méthode par chromatographie liquide à haute performance (HPLC)
Düngemittel - Bestimmung von N-(2-Nitrophenyl)Phosphorsäure-Triamid (2-NPT) in Harnstoff und harnstoffhaltigen Düngemitteln - Verfahren mit Hochleistungs-Flüssigchromatographie (HPLC) This European Standard was approved by CEN on 24 September 2011.
CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management Centre has the same status as the official versions.
CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland and United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION EUROPÄISCHES KOMITEE FÜR NORMUNG
Management Centre:
Avenue Marnix 17,
B-1000 Brussels © 2011 CEN All rights of exploitation in any form and by any means reserved worldwide for CEN national Members. Ref. No. EN 16075:2011: ESIST EN 16075:2012
Statistical results of the inter-laboratory test .9Bibliography . 10 SIST EN 16075:2012
EN ISO 3696:1995, Water for analytical laboratory use — Specification and test
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