EN ISO 17512-2:2020

SLOVENSKI STANDARD
01-november-2020
Kakovost tal - Izogibalni preskus za določanje kakovosti tal in učinkov kemikalij na
obnašanje - 2. del: Preskus s Folsomia candida iz rodu skakačev (Collembola)
(ISO 17512-2:2011)
Soil quality - Avoidance test for determining the quality of soils and effects of chemicals
on behaviour - Part 2: Test with collembolans (Folsomia candida) (ISO 17512-2:2011)
Bodenbeschaffenheit - Vermeidungsprüfung zur Bestimmung der Bodenbeschaffenheit
und der Auswirkungen von Chemikalien auf das Verhalten - Teil 2: Prüfung mit
Collembolen (Folsomia candida) (ISO 17512-2:2011)
Qualité du sol - Essai d'évitement pour contrôler la qualité des sols et les effets des
produits chimiques sur le comportement - Partie 2: Essai avec des collemboles
(Folsomia candida) (ISO 17512-2:2011)
Ta slovenski standard je istoveten z: EN ISO 17512-2:2020
ICS:
13.080.30 Biološke lastnosti tal Biological properties of soils
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

EN ISO 17512-2
EUROPEAN STANDARD
NORME EUROPÉENNE
May 2020
EUROPÄISCHE NORM
ICS 13.080.30
English Version
Soil quality - Avoidance test for determining the quality of
soils and effects of chemicals on behaviour - Part 2: Test
with collembolans (Folsomia candida) (ISO 17512-2:2011)
Qualité du sol - Essai d'évitement pour contrôler la Bodenbeschaffenheit - Vermeidungsprüfung zur
qualité des sols et les effets des produits chimiques sur Bestimmung der Bodenbeschaffenheit und der
le comportement - Partie 2: Essai avec des collemboles Auswirkungen von Chemikalien auf das Verhalten -
(Folsomia candida) (ISO 17512-2:2011) Teil 2: Prüfung mit Collembolen (Folsomia candida)
(ISO 17512-2:2011)
This European Standard was approved by CEN on 13 April 2020.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this
European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by
translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management
Centre has the same status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and
United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2020 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 17512-2:2020 E
worldwide for CEN national Members.

Contents Page
European foreword . 3

European foreword
The text of ISO 17512-2:2011 has been prepared by Technical Committee ISO/TC 190 "Soil Quality” of
the International Organization for Standardization (ISO) and has been taken over as EN ISO 17512-
2:2020 by Technical Committee CEN/TC 444 “Environmental characterization of solid matrices” the
secretariat of which is held by NEN.
This European Standard shall be given the status of a national standard, either by publication of an
identical text or by endorsement, at the latest by November 2020, and conflicting national standards
shall be withdrawn at the latest by November 2020.
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. CEN shall not be held responsible for identifying any or all such patent rights.
According to the CEN-CENELEC Internal Regulations, the national standards organizations of the
following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria,
Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland,
Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Republic of
North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and the
United Kingdom.
Endorsement notice
The text of ISO 17512-2:2011 has been approved by CEN as EN ISO 17512-2:2020 without any
modification.
INTERNATIONAL  ISO
STANDARD 17512-2
First edition
2011-10-01
Soil quality — Avoidance test for
determining the quality of soils and
effects of chemicals on behaviour —
Part 2:
Test with collembolans (Folsomia candida)
Qualité du sol — Essai d’évitement pour contrôler la qualité des sols et
les effets des produits chimiques sur le comportement —
Partie 2: Essai avec des collemboles (Folsomia candida)
Reference number
ISO 17512-2:2011(E)
©
ISO 2011
ISO 17512-2:2011(E)
©  ISO 2011
All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized in any form or by any means,
electronic or mechanical, including photocopying and microfilm, without permission in writing from either ISO at the address below or ISO’s
member body in the country of the requester.
ISO copyright office
Case postale 56 • CH-1211 Geneva 20
Tel. + 41 22 749 01 11
Fax + 41 22 749 09 47
E-mail copyright@iso.org
Web www.iso.org
Published in Switzerland
ii © ISO 2011 – All rights reserved

ISO 17512-2:2011(E)
Contents Page
Foreword .iv
Introduction .v
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Principle . 2
5 Reagents and materials . 2
6 Apparatus . 3
7 Procedure . 4
7.1 Appropriate concentration/dilution range . 4
7.2 Testing of soils . 4
7.3 Reference substance . 4
7.4 Validity criteria . 5
8 Calculation and expression of results . 5
8.1 General . 5
8.2 Calculation of the percentage of avoidance . 5
8.3 NOEC/LOEC calculation . 5
8.4 EC calculation . 6
x
9 Test report . 6
Annex A (informative) Techniques for rearing and breeding of Collembola . 7
Annex B (informative) Test chambers . 9
Annex C (normative) Testing of chemicals in the avoidance test .10
Annex D (normative) Determination of the water-holding capacity .12
Bibliography .13
© ISO 2011 – All rights reserved  iii

ISO 17512-2:2011(E)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards bodies
(ISO member bodies). The work of preparing International Standards is normally carried out through ISO
technical committees. Each member body interested in a subject for which a technical committee has been
established has the right to be represented on that committee. International organizations, governmental and
non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely with the International
Electrotechnical Commission (IEC) on all matters of electrotechnical standardization.
International Standards are drafted in accordance with the rules given in the ISO/IEC Directives, Part 2.
The main task of technical committees is to prepare International Standards. Draft International Standards
adopted by the technical committees are circulated to the member bodies for voting. Publication as an
International Standard requires approval by at least 75 % of the member bodies casting a vote.
Attention is drawn to the possibility that some of the elements of this document may be the subject of patent
rights. ISO shall not be held responsible for identifying any or all such patent rights.
ISO 17512-2 was prepared by Technical Committee ISO/TC 190,  Soil quality, Subcommittee SC 4,
Biological methods.
ISO 17512 consists of the following parts, under the general title Soil quality — Avoidance test for determining
the quality of soils and effects of chemicals on behaviour:
—  Part 1: Test with earthworms (Eisenia fetida and Eisenia andrei)
—  Part 2: Test with collembolans (Folsomia candida)
iv © ISO 2011 – All rights reserved

ISO 17512-2:2011(E)
Introduction
The use of the avoidance behaviour of soil invertebrates as an indicator of unfavourable conditions allows
a preliminary assessment of contaminated soils in a short period of time, with a high degree of sensitivity.
Being rapid, cost-effective and ecologically relevant, the avoidance tests with earthworms were proposed to
complement conventional chemical analysis. Supporting the results obtained in the chronic tests, the avoidance
bioassays can be used as a first screening tool in the assessment of the habitat function of soils. Considering
the fact that the avoidance response of soil invertebrates differs between species due to their distinct sensitivity
to contaminants and modes of exposure, it is recommended to standardize a second rapid cost-effective and
ecologically relevant avoidance bioassay.
Springtails have shown a distinct sensitivity towards several contaminants when compared with earthworms,
[1][2]
complementing the information obtained in the avoidance tests with earthworms . Until now, the species
Folsomia candida has been the most commonly used collembolan test species due to a great facility to keep
[3]
laboratory cultures and due to their high locomotor ability . Folsomia candida is considered to be a hemi-
edaphic species, meaning that it lives mainly in the soil. Furthermore, this species is already used in ISO 11267.
© ISO 2011 – All rights reserved  v

INTERNATIONAL STANDARD ISO 17512-2:2011(E)
Soil quality — Avoidance test for determining the quality
of soils and effects of chemicals on behaviour —
Part 2:
Test with collembolans (Folsomia candida)
1 Scope
This part of ISO 17512 specifies a rapid screening method for evaluating the habitat function of soils based on
the avoidance behaviour of springtails.
The test is a rapid method that reflects the bioavailability of contaminants in natural soils and substances
spiked into soils to Folsomia candida. In both cases, it is possible to establish a dose-response-relationship.
The avoidance behaviour of the springtails is the measurement endpoint of the test. This test is not intended to
replace the Collembola reproduction test.
2 Normative references
The following r
...