EN 13506:2001

2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.Water quality - Determination of mercury by atomic fluorescence spectrometryVSHNWURPHWULMRQualité de l'eau - Dosage du mercure par spectrométrie de fluorescence atomiqueWasserbeschaffenheit - Bestimmung von Quecksilber mittels Atomfluoreszenzspektrometrie13.060.50VQRYLExamination of water for chemical substancesICS:SIST EN 13506:2003enTa slovenski standard je istoveten z:EN 13506:200101-maj-2003SIST EN 13506:2003SLOVENSKI
STANDARDSIST EN ISO 17852:20081DGRPHãþD

EUROPEAN STANDARDNORME EUROPÉENNEEUROPÄISCHE NORMEN 13506November 2001ICS 13.060.50English versionWater quality - Determination of mercury by atomic fluorescencespectrometryQualité de l'eau - Dosage du mercure par spectrométrie defluorescence atomiqueWasserbeschaffenheit - Bestimmung von Quecksilbermittels AtomfluoreszenzspektrometrieThis European Standard was approved by CEN on 4 October 2001.CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this EuropeanStandard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such nationalstandards may be obtained on application to the Management Centre or to any CEN member.This European Standard exists in three official versions (English, French, German). A version in any other language made by translationunder the responsibility of a CEN member into its own language and notified to the Management Centre has the same status as the officialversions.CEN members are the national standards bodies of Austria, Belgium, Czech Republic, Denmark, Finland, France, Germany, Greece,Iceland, Ireland, Italy, Luxembourg, Netherlands, Norway, Portugal, Spain, Sweden, Switzerland and United Kingdom.EUROPEAN COMMITTEE FOR STANDARDIZATIONCOMITÉ EUROPÉEN DE NORMALISATIONEUROPÄISCHES KOMITEE FÜR NORMUNGManagement Centre: rue de Stassart, 36
B-1050 Brussels© 2001 CENAll rights of exploitation in any form and by any means reservedworldwide for CEN national Members.Ref. No. EN 13506:2001 E

Additional Information.13Annex B (informative)
Schematic Block Diagram.14Annex C (informative)
Availability of Reagents etc.15Bibliography.16

(K2Cr2O7) = 0,05 g/lDissolve 50 mg of K2Cr2O7 in 50 ml nitric acid (5.7) and dilute to 1 000 ml with water (5.2).5.9 Hydrochloric acid (HCl), w(HCl) = 120 g/kgDilute 167 ml of high purity hydrochloric acid w(HCl) = 360 g/kg ( (HCl) = 1,19 g/ml) to 500 ml with water (5.2).5.10 Tin(II)chloride solution,
(SnCl2
2 H2O) = 20 g/lAdd 10,0 g of tin(II)chloride dihydrate to 150 ml of hydrochloric acid (5.9). Heat to dissolve. Dilute to 500 ml withwater (5.2). To remove any traces of mercury, bubble the solution with argon, nitrogen or air e.g. at a flow rate of2 l/min for 15 min.NOTEThe hydrochloric acid used to prepare this solution can be analytical grade since any mercury present will beremoved on bubbling.5.11 Reagent blankFor each 100 ml, prepare a solution containing 15 ml of hydrochloric acid (5.9) and 2 ml of potassium bromide -potassium bromate reagent (5.5). Add 100 µl of ascorbic acid solution (5.6) for each 10 ml prepared [5]. It isessential that the same reagents used for sample and standard preparation are used for preparation of the reagentblank. Treat the reagent blank like a sample.NOTEOn the continuous flow system, the reagent blank solution is run as background for automatic blank subtraction.This solution can contain trace levels of detectable amounts of mercury.5.12 Mercury standard solutions5.12.1 Mercury stock solution A,
(Hg) = 1 000 mg/lUse a commercially available quantitative standard solution. This solution is stable for at least six months.Alternatively use a stock solution prepared from ultra high purity grade chemicals (99,99 / 99,999 % (mass fraction)pure). Dissolve 0,1354 g of mercury(II)chloride HgCl2 in 20 ml water (5.2). Add 5 ml of nitric acid (5.7) and dilute to100 ml.WARNING — Do not dry the inorganic salt, it is highly toxic.5.12.2 Mercury stock solution B, (Hg) = 10 mg/lDilute 1 ml of stock solution A (5.12.1) with water (5.2) to approximately 20 ml. Add 2 ml of potassium bromide -potassium bromate reagent (5.5) and 15 ml of hydrochloric acid (5.9) and dilute to 100 ml in a borosilicatevolumetric flask with water. Prepare weekly.

(Hg) = 100 µg/lDilute 1 ml of stock solution B (5.12.2) to 100 ml with reagent blank (5.11) in a borosilicate flask.Prepare the solution on the day of use.5.12.4 Mercury stock solution D,
(Hg) = 1 µg/lDilute 1 ml of stock solution C (5.12.3) to 100 ml with reagent blank (5.11) in a borosilicate flask.Prepare the solution freshly before each series of measurements.5.12.5 Calibration standardsPrepare a minimum of five mercury calibration standards spanning the concentration range of interest by serialdilution of the stock solution D (5.12.4). Each calibration standard shall contain 15 ml of hydrochloric acid (5.9) and2 ml of potassium bromide - potassium bromate reagent (5.5) per 100 ml in borosilicate volumetric flasks. Plasticflasks should not be used if they are permeable to mercury(0) vapour. Prepare daily.The matrix of the reagent blank solution shall be identical to that of the standard solutions.For the concentration range from 10 ng/l to 100 ng/l, for example, proceed as follows: Prepare 5 calibration standards of concentrations 10 ng/l, 30 ng/l, 50 ng/l, 70 ng/l and 100 ng/l by taking 1 ml,3 ml, 5 ml, 7 ml and 10 ml respectively of mercury stock solution D (5.12.4) and diluting accurately to 100 mlwith reagent blank (5.11).For the concentration range from 2 ng/l to 20 ng/l, for example, proceed as follows: Prepare a working stock solution of 100 ng/l by taking 10 ml of mercury stock solution D (5.12.4) and diluting itaccurately to 100 ml with reagent blank (5.11). Prepare daily. From
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