EN 13946:2014

2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.Wasserbeschaffenheit - Anleitung zur Probenahme und Probenaufbereitung von benthischen Kieselalgen aus Fließgewässern und SeenQualité de l'eau - Guide pour l'échantillonnage en routine et le prétraitement des diatomées benthiques de rivières et de plans d'eauWater quality - Guidance for the routine sampling and preparation of benthic diatoms from rivers and lakes13.060.70Preiskava bioloških lastnosti vodeExamination of biological properties of water13.060.10Voda iz naravnih virovWater of natural resourcesICS:Ta slovenski standard je istoveten z:EN 13946:2014SIST EN 13946:2014en,fr,de01-maj-2014SIST EN 13946:2014SLOVENSKI
STANDARDSIST EN 13946:20031DGRPHãþD

EUROPEAN STANDARD NORME EUROPÉENNE EUROPÄISCHE NORM
EN 13946
March 2014 ICS 13.060.70 Supersedes EN 13946:2003English Version
Water quality - Guidance for the routine sampling and preparation of benthic diatoms from rivers and lakes
Qualité de l'eau - Guide pour l'échantillonnage en routine et le prétraitement des diatomées benthiques de rivières et de plans d'eau
Wasserbeschaffenheit - Anleitung zur Probenahme und Probenaufbereitung von benthischen Kieselalgen aus Fließgewässern und Seen This European Standard was approved by CEN on 20 December 2013.
CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management Centre has the same status as the official versions.
CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and United Kingdom.
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Foreword .3 Introduction .4 1 Scope .5 2 Terms and definitions .5 3 Principle .6 4 Equipment .6 4.1 Equipment for field sampling .6 4.2 Laboratory equipment .6 5 Reagents .6 6 Procedure .8 6.1 Choice of substratum .8 6.2 Sample site selection .8 6.3 Sampling methods .8 6.3.1 Moveable natural hard surfaces .8 6.3.2 Method for sampling vertical man-made surfaces in situ .9 6.3.3 Use of artificial substrata . 10 6.3.4 Sample collection from submerged macrophytes and macroalgae . 10 6.3.5 Sample collection from emergent macrophytes . 10 6.4 Preparation prior to microscopic examination . 11 6.4.1 Preservation and preliminary laboratory treatment . 11 6.4.2 Methods for cleaning diatoms . 11 6.4.3 Preparation of permanent slides . 11 Annex A (informative)
Methods for cleaning diatoms for microscopic examination . 13 A.1 General . 13 A.2 Method 1: Hot hydrogen peroxide . 13 A.2.1 Apparatus . 13 A.2.2 Reagents . 13 A.2.3 Procedure . 13 A.3 Method 2: Cold hydrogen peroxide . 14 A.3.1 Apparatus and reagents . 14 A.3.2 Procedure . 14 A.4 Method 3: Hot hydrogen peroxide with potassium dichromate . 14 A.4.1 Apparatus . 14 A.4.2 Reagents . 14 A.4.3 Procedure . 14 A.5 Method 4: Cold acid (permanganate) method of cleaning . 15 A.5.1 Apparatus . 15 A.5.2 Reagents . 15 A.5.3 Procedure . 15 Bibliography . 17
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