EN ISO 18763:2020

SLOVENSKI STANDARD
01-november-2020
Kakovost tal - Določanje toksičnih učinkov onesnaževal na kalivost in zgodnjo
rast višjih rastlin (ISO 18763:2016)
Soil quality - Determination of the toxic effects of pollutants on germination and early
growth of higher plants (ISO 18763:2016)
Bodenbeschaffenheit - Bestimmung der toxischen Wirkung von Schadstoffen auf die
Keimung und das frühe Wachstum höherer Pflanzen (ISO 18763:2016)
Qualité du sol - Détermination des effets toxiques des polluants sur la germination et les
premiers stades de croissance des végétaux supérieurs (ISO 18763:2016)
Ta slovenski standard je istoveten z: EN ISO 18763:2020
ICS:
13.080.30 Biološke lastnosti tal Biological properties of soils
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

EN ISO 18763
EUROPEAN STANDARD
NORME EUROPÉENNE
April 2020
EUROPÄISCHE NORM
ICS 13.080.30
English Version
Soil quality - Determination of the toxic effects of
pollutants on germination and early growth of higher
plants (ISO 18763:2016)
Qualité du sol - Détermination des effets toxiques des Bodenbeschaffenheit - Bestimmung der toxischen
polluants sur la germination et la croissance primaire Wirkung von Schadstoffen auf die Keimung und das
des plantes supérieures (ISO 18763:2016) frühe Wachstum höherer Pflanzen (ISO 18763:2016)
This European Standard was approved by CEN on 13 April 2020.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this
European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by
translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management
Centre has the same status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and
United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2020 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 18763:2020 E
worldwide for CEN national Members.

Contents Page
European foreword . 3

European foreword
The text of ISO 18763:2016 has been prepared by Technical Committee ISO/TC 190 "Soil quality” of the
International Organization for Standardization (ISO) and has been taken over as EN ISO 18763:2020 by
Technical Committee CEN/TC 444 “Environmental characterization of solid matrices” the secretariat of
which is held by NEN.
This European Standard shall be given the status of a national standard, either by publication of an
identical text or by endorsement, at the latest by October 2020, and conflicting national standards shall
be withdrawn at the latest by October 2020.
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. CEN shall not be held responsible for identifying any or all such patent rights.
According to the CEN-CENELEC Internal Regulations, the national standards organizations of the
following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria,
Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland,
Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Republic of
North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and the
United Kingdom.
Endorsement notice
The text of ISO 18763:2016 has been approved by CEN as EN ISO 18763:2020 without any modification.

INTERNATIONAL ISO
STANDARD 18763
First edition
2016-07-01
Soil quality — Determination of
the toxic effects of pollutants on
germination and early growth of
higher plants
Qualité du sol — Détermination des effets toxiques des polluants sur
la germination et la croissance primaire des plantes supérieures
Reference number
ISO 18763:2016(E)
©
ISO 2016
ISO 18763:2016(E)
© ISO 2016, Published in Switzerland
All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized otherwise in any form
or by any means, electronic or mechanical, including photocopying, or posting on the internet or an intranet, without prior
written permission. Permission can be requested from either ISO at the address below or ISO’s member body in the country of
the requester.
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Tel. +41 22 749 01 11
Fax +41 22 749 09 47
copyright@iso.org
www.iso.org
ii © ISO 2016 – All rights reserved

ISO 18763:2016(E)
Contents Page
Foreword .iv
Introduction .v
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Principle . 3
5 Reagents, test organisms and media . 3
6 Apparatus and materials. 4
7 Treatment and preparation of samples . 6
8 Procedure. 6
9 Measurement . 8
10 Calculation of the percentage inhibition . 9
11 Reference chemical .10
12 Precision .10
13 Validity criteria .11
14 Test report .11
Annex A (informative) Application of the phytotoxicity test in transparent test plates on
natural and artificial soils and soil materials, with different plant species .12
Annex B (informative) Assemblage of test plates for the phytotoxicity test .14
Annex C (informative) International interlaboratory comparisons on the phytotoxicity test .15
Bibliography .20
ISO 18763:2016(E)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards
bodies (ISO member bodies). The work of preparing International Standards is normally carried out
through ISO technical committees. Each member body interested in a subject for which a technical
committee has been established has the right to be represented on that committee. International
organizations, governmental and non-governmental, in liaison with ISO, also take part in the work.
ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of
electrotechnical standardization.
The procedures used to develop this document and those intended for its further maintenance are
described in the ISO/IEC Directives, Part 1. In particular the different approval criteria needed for the
different types of ISO documents should be noted. This document was drafted in accordance with the
editorial rules of the ISO/IEC Directives, Part 2 (see www.iso.org/directives).
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. ISO shall not be held responsible for identifying any or all such patent rights. Details of
any patent rights identified during the development of the document will be in the Introduction and/or
on the ISO list of patent declarations received (see www.iso.org/patents).
Any trade name used in this document is information given for the convenience of users and does not
constitute an endorsement.
For an explanation on the meaning of ISO specific terms and expressions related to conformity
assessment, as well as information about ISO’s adherence to the WTO principles in the Technical
Barriers to Trade (TBT) see the following URL: Foreword - Supplementary information
The committee responsible for this document is ISO/TC 190, Soil quality, Subcommittee SC 4, Biological
methods.
iv © ISO 2016 – All rights reserved

ISO 18763:2016(E)
Introduction
Ecotoxicological testing of test soils or waste materials to be disposed on soil are required to assess
the potential environmental risk resulting from soil pollution or the disposal of wastes such as sewage
sludge on farmland. There is also a need to monitor the quality of soil after reclamation of industrial
sites. Therefore, a very practical and rapid germination and growth test has been developed based on
seed germination and seedling growth in controlled environmental conditions.
The assay, which does not require any pretreatment of the seeds, is performed in “transparent test plates”,
incubated vertically, to allow the roots and the shoots of the germinated seeds to be seen. After 72 h
exposure, a picture of the transparent test plates is taken and can be analysed “by image analysis” for
multiple endpoints, such as percentage of seed germination and of length of roots and shoots. To account
for the plant species variability in sensitivity, the assays are performed with the seeds of three plant
species: one monocotyl (Sorghum saccharatum) and two dicotyls (Lepidium sativum and Sinapis alba).
A major advantage of this test is that after the shooting and storing of the pictures of the test plates, the
measurements by image analysis can be postponed to any appropriate timing.
Reference or standard soils can be used as negative controls, such as, for example, the ISO standard
artificial soil according to ISO 11269-1 and ISO 11269-2.
Commercially available seeds, with a shelf life longer than one year, allow the use of this test at any time
of the year.
Two International interlaboratory comparisons demonstrated that the test provides good results.
A substantial number of studies report data on the application of this test on various types of soils and
soil materials with several types of plant species.
INTERNATIONAL STANDARD ISO 18763:2016(E)
Soil quality — Determination of the toxic effects of
pollutants on germination and early growth of higher plants
1 Scope
This International Standard describes a technique for determining the effects of soil and soil-related
material
...