EN 17204:2019

SLOVENSKI STANDARD
01-julij-2019
Kakovost vode - Navodilo za analizo mezozooplanktona v morskih in brakičnih
vodah
Water quality - Guidance on analysis of mesozooplankton from marine and brackish
water
Wasserbeschaffenheit - Anleitung zur Analyse von Zooplankton aus marinen und
brackigen Gewässern
Qualité de l'eau - Document d'orientation sur l'analyse du mésozooplancton dans les
eaux marines et saumâtres
Ta slovenski standard je istoveten z: EN 17204:2019
ICS:
13.060.10 Voda iz naravnih virov Water of natural resources
13.060.70 Preiskava bioloških lastnosti Examination of biological
vode properties of water
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

EN 17204
EUROPEAN STANDARD
NORME EUROPÉENNE
April 2019
EUROPÄISCHE NORM
ICS 13.060.70
English Version
Water quality - Guidance on analysis of mesozooplankton
from marine and brackish waters
Qualité de l'eau - Document d'orientation sur l'analyse Wasserbeschaffenheit - Anleitung zur Analyse von
du mésozooplancton dans les eaux marines et Zooplankton aus marinen und brackigen Gewässern
saumâtres
This European Standard was approved by CEN on 11 February 2019.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this
European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by
translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management
Centre has the same status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania,
Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland,
Turkey and United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2019 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN 17204:2019 E
worldwide for CEN national Members.

Contents Page
European foreword . 4
Introduction . 5
1 Scope . 6
2 Normative references . 6
3 Terms and definitions . 6
4 Principle . 7
5 Equipment . 7
6 Preservatives and other chemicals . 8
7 Procedure. 9
7.1 Sample and sub-sample preparation . 9
7.2 Species identification and counting . 10
7.2.1 General . 10
7.2.2 Statistical requirements for counting . 11
7.2.3 Calculation of abundance . 12
7.3 Biomass determination . 12
7.3.1 General . 12
7.3.2 Calibration of the eyepiece micrometre, counting-graticule and image analysis
software . 12
7.3.3 Calculation of carbon biomass based on length measurements . 13
7.3.4 Calculation of biomass based on carbon conversion factors . 13
7.3.5 Calculation of biomass based on standard size classes . 14
7.3.6 Calculation of biomass based on individual weight factors . 14
8 Reporting . 14
9 Quality assurance . 15
9.1 General . 15
9.2 Photo documentation . 15
9.3 Reference collections . 15
9.4 Long term storage of samples . 16
Annex A (informative) Advise for length measurements of some selected zooplankton taxa
and taxonomic groups L [µm] . 17
Annex B (informative) Coefficients a and b for biomass calculations based on length
measurements . 19
Annex C (informative) Biomass conversion factors [µg C/Ind.] . 20
Annex D (informative) Individual wet weights of selected zooplankton taxa for calculations
of biomass . 22
Annex E (informative) Examples of sample preparation devices . 27
E.1 Counting chambers . 27
E.1.1 General . 27
E.1.2 Counting Chamber according to Bogorov . 27
E.1.3 Ward counting wheel . 27
E.1.4 Tubular Plankton Chambers . 28
E.1.5 Utermöhl sedimentation and counting chamber . 28
E.1.6 Dolfuss counting chamber . 29
E.2 Sample splitters . 29
Bibliography . 31

European foreword
This document (EN 17204:2019) has been prepared by Technical Committee CEN/TC 230 “Water
analysis”, the secretariat of which is held by DIN.
This European Standard shall be given the status of a national standard, either by publication of an
identical text or by endorsement, at the latest by October 2019, and conflicting national standards shall
be withdrawn at the latest by October 2019.
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. CEN shall not be held responsible for identifying any or all such patent rights.
According to the CEN-CENELEC Internal Regulations, the national standards organisations of the
following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria,
Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia,
France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta,
Netherlands, Norway, Poland, Portugal, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland,
Turkey and the United Kingdom.
Introduction
WARNING — Person using this European Standard should be familiar with normal laboratory
practice. This standard does not purport to address all of the safety problems, if any, associated
with its use. It is the responsibility of the user to establish appropriate health and safety
practices and to ensure compliance with any national and international regulatory conditions.
Mesozooplankton constitute an important part of zooplankton in the pelagic food webs, since these are
the organisms representing the link between primary producers and higher trophic levels.
Mesozooplankton community structure and productivity can be affected by changes in phytoplankton
stocks, species/size composition and phenology. Further, alterations in mesozooplankton can influence
prey availability for zooplanktivores and, thus, fish stock recruitment, as well as sedimentation of the
primary production, which, in turn, may affect food supply to benthic animals and oxygen levels in the
bottom water. [11].
Mesozooplankton comprise a large number of species within a range of total lengths of about 0,2 mm to
20 mm. The main groups are rotifers (Rotatoria), crustacean holozooplankton and merozooplanktonic
larvae of other taxa such as echinoderms, bivalves and crustaceans. Small hydromedusae, ctenophores,
chaetognaths, appendicularians, doliolids, fish eggs and larvae are also considered as part of the
mesozooplanktonic fauna in marine waters. As most protozooplankton species are smaller than 0,2 mm
these are not considered part of the mesozooplankton and hence procedures for sampling and
enumeration of these species are not included in this standard.
For sampling, preservation and storage of mesozooplankton see EN 17218:2019
1 Scope
This document specifies a procedure for analysing mesozooplankton in marine and brackish waters.
The procedure comprises how to identify and enumerate mesozooplankton to estimate quantitative
information on diversity, abundance and biomass with regard to spatial distribution and long-term
temporal trends for a given body of water.
2 Normative references
The following documents are referred to in the text in such a way that some or all of their content
constitutes requirements of this document. For dated references, only the edition cited applies. For
undated references, the latest edition of the referenced document (including any amendments) applies.
EN 17218:2019, Water quality — Guidance for the sampling of mesozooplankton from marine and
brackish waters using mesh
3 Terms and definitions
For the purposes of this document, the following terms and definitions apply.
ISO and IEC maintain terminological databases for use in standardization at the following addresses:
— IEC Electropedia: available at http://www.electropedia.org/
— ISO Online browsing platform: available at http://www.iso.org/obp
3.1
biomass
total mass of living organic material in a given body of water
-3
Note 1 to entry: Unit: g m .
[SOURCE: ISO 6107-3:1993, definition 12, modified – Added note1 to entry]
3.2
metazoan
multicellular animal that develops from embryos
3.3
plankton
organisms drifting or suspended in water, consisting chiefly of minute plants or animals, but including
larger forms having only weak powers of locomotion
[SOURCE: ISO 6107-5:2004, definition 41]
3.4
zooplankton
animals present in plankton
[SOURCE: ISO 6107-5:2004, definition 49]
3.5
merozooplankton
zooplankton that occurs in the plankton for only a part of their life cycle, usually the larvae stage
3.6
mesozooplankton
zooplankton of 0,2 mm to 20 mm
...