EN ISO 9377-2:2000

SLOVENSKI STANDARD
01-december-2001
.DNRYRVWYRGH'RORþHYDQMHLQGHNVDPLQHUDOQLKROMGHO0HWRGD]XSRUDER
HNVWUDNFLMHVWRSLORPLQSOLQVNHNURPDWRJUDILMH,62
Water quality - Determination of hydrocarbon oil index - Part 2: Method using solvent
extraction and gas chromatography (ISO 9377-2:2000)
Wasserbeschaffenheit - Bestimmung des Kohlenwasserstoff-Index - Teil 2: Verfahren
nach Lösemittelextraktion und Gaschromatographie (ISO 9377-2:2000)
Qualité de l'eau - Détermination de l'indice hydrocarbure - Partie 2: Méthode par
extraction au solvant et chromatographie en phase gazeuse (ISO 9377-2:2000)
Ta slovenski standard je istoveten z: EN ISO 9377-2:2000
ICS:
13.060.50 3UHLVNDYDYRGHQDNHPLþQH Examination of water for
VQRYL chemical substances
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

EUROPEAN STANDARD
EN ISO 9377-2
NORME EUROPÉENNE
EUROPÄISCHE NORM
October 2000
ICS 13.060.50
English version
Water quality - Determination of hydrocarbon oil index - Part 2:
Method using solvent extraction and gas chromatography (ISO
9377-2:2000)
Qualité de l'eau - Détermination de l'indice hydrocarbure - Wasserbeschaffenheit - Bestimmung des
Partie 2: Méthode par extraction au solvant et Kohlenwasserstoff-Index - Teil 2: Verfahren nach
chromatographie en phase gazeuse (ISO 9377-2:2000) Lösemittelextraktion und Gaschromatographie (ISO 9377-
2:2000)
This European Standard was approved by CEN on 4 October 2000.
CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European
Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national
standards may be obtained on application to the Management Centre or to any CEN member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by translation
under the responsibility of a CEN member into its own language and notified to the Management Centre has the same status as the official
versions.
CEN members are the national standards bodies of Austria, Belgium, Czech Republic, Denmark, Finland, France, Germany, Greece,
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© 2000 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 9377-2:2000 E
worldwide for CEN national Members.

Page 2
CORRECTED 2001-03-28
Foreword
The text of the International Standard ISO 9377-2:2000 has been prepared by Technical
Committee ISO/TC 147 "Water quality" in collaboration with Technical Committee CEN/TC
230 "Water analysis", the secretariat of which is held by DIN.
This European Standard shall be given the status of a national standard, either by publication
of an identical text or by endorsement, at the latest by April 2001, and conflicting national
standards shall be withdrawn at the latest by April 2001.
According to the CEN/CENELEC Internal Regulations, the national standards organizations of
the following countries are bound to implement this European Standard: Austria, Belgium,
Czech Republic, Denmark, Finland, France, Germany, Greece, Iceland, Ireland, Italy,
Luxembourg, Netherlands, Norway, Portugal, Spain, Sweden, Switzerland and the United
Kingdom.
Endorsement notice
The text of the International Standard ISO 9377-2:2000 was approved by CEN as a European
Standard without any modification.
NOTE: Normative references to International Standards are listed in annex ZA (normative).

Page 3
Annex ZA (normative)
Normative references to international publications
with their relevant European publications
This European Standard incorporates by dated or undated reference, provisions from other
publications. These normative references are cited at the appropriate places in the text and the
publications are listed hereafter. For dated references, subsequent amendments to or revisions
of any of these publications apply to this European Standard only when incorporated in it by
amendment or revision. For undated references the latest edition of the publication referred to
applies (including amendments).
NOTE Where an International Publication has been modified by common modifications, indicated by
(mod.), the relevant EN/HD applies.
Publication Year Title EN/HD Year
ISO 5667-3 1994 Water quality - Sampling - Part 3: EN ISO 5667-3 1995
Guidance on the preservation and
handling of samples
INTERNATIONAL ISO
STANDARD 9377-2
First edition
2000-10-15
Water quality — Determination of
hydrocarbon oil index —
Part 2:
Method using solvent extraction and gas
chromatography
Qualité de l'eau — Détermination de l'indice hydrocarbure —
Partie 2: Méthode par extraction au solvant et chromatographie en phase
gazeuse
Reference number
ISO 9377-2:2000(E)
©
ISO 2000
ISO 9377-2:2000(E)
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ii © ISO 2000 – All rights reserved

ISO 9377-2:2000(E)
Contents Page
Foreword.iv
1 Scope .1
2 Normative references .1
3 Term and definition .1
4 Interferences .2
5 Principle.2
6 Reagents.2
7 Apparatus .4
8 Sampling and sample preservation .5
9 Procedure .5
10 Test report .9
11 Precision.10
Annex A (informative) Example of a column and a microseparator .11
Annex B (informative) Examples of gas chromatograms of mineral oil standard and water samples .13
Annex C (informative) Determination of boiling range of a mineral oil from the gas chromatogram .18
Bibliography.19
ISO 9377-2:2000(E)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards bodies (ISO
member bodies). The work of preparing International Standards is normally carried out through ISO technical
committees. Each member body interested in a subject for which a technical committee has been established has
the right to be represented on that committee. International organizations, governmental and non-governmental, in
liaison with ISO, also take part in the work. ISO collaborates closely with the International Electrotechnical
Commission (IEC) on all matters of electrotechnical standardization.
International Standards are drafted in accordance with the rules given in the ISO/IEC Directives, Part 3.
Draft International Standards adopted by the technical committees are circulated to the member bodies for voting.
Publication as an International Standard requires approval by at least 75 % of the member bodies casting a vote.
Attention is drawn to the possibility that some of the elements of this part of ISO 9377 may be the subject of patent
rights. ISO shall not be held responsible for identifying any or all such patent rights.
International Standard ISO 9377-2 was prepared by Technical Committee ISO/TC 147, Water quality,
Subcommittee SC 2, Physical, chemical and biochemical methods.
ISO 9377 consists of the following parts, under the general title Water quality — Determination of hydrocarbon oil
index:
� Part 1: Method using solvent extraction and gravimetry
� Part 2: Method using solvent extraction and gas chromatography
Annexes A, B and C of this part of ISO 9377 are for information only.
iv © ISO 2000 – All rights reserved

INTERNATIONAL STANDARD ISO 9377-2:2000(E)
Water quality — Determination of hydrocarbon oil index —
Part 2:
Method using solvent extraction and gas chromatography
1 Scope
This part of ISO 9377 specifies a method for the determination of the hydrocarbon oil index in waters by means of
gas chromatography. The method is suitable for surface water, waste water and water from sewage treatment
plants and allows the determination of a hydrocarbon oil index in concentrations above 0,1 mg/l.
The method is not applicable to the quantitative determination of the content of volatile mineral oil. However, on the
basis of the peak pattern of the gas chromatogram, certain qualitative information on the composition of the mineral
oil contamination can be derived.
NOTE 1 For the determination of the mineral-oil content of soils and sediment, see ISO/TR 11046.
NOTE 2 The mass concentration of animal and vegetable fat in the test sample should not exceed 150 mg/l, because at
higher values the adsorption capacity of the clean-up column packing may not be sufficient.
NOTE 3 In the case of highly polluted waste water, especially if containing a high amount of surfactants, a loss in recovery
may occur.
2 Normative references
The following normative documents contain provisions which, through reference in this text, constitute provisions of
this part of ISO 9377. For dated references, subsequent amendments to, or revisions of, any of these publications
do not apply. However, parties to agreements based on this part of ISO 9377 are encouraged to investigate the
possibility of applying the most recent editions of the normative documents indicated below. For undated
references, the latest edition of the normative document referred to applies. Members of ISO and IEC maintain
registers of currently valid International Standards.
ISO 5667-3:1994, Water quality — Sampling — Part 3: Guidance on the preservation and handling of samples.
ISO 8466-1:1990, Water quality — Calibration and evaluation of analytical methods and estimation of performance
characteristics — Part 1: Statistical evaluation of the linear calibration function.
3 Term and definition
For the purposes of this part of ISO 9377, the following term and definition applies.
ISO 9377-2:2000(E)
3.1
hydrocarbon oil index by GC-FID
the sum of concentrations of compounds extractable with a hydrocarbon solvent, boiling point between 36 �Cand
1)
69 �C, not adsorbed on Florisil and which may be chromatographed with retention times between those of
n-decane (C H ) and n-tetracontane (C H )
10 22 40 82
NOTE Substances complying with this definition are long-chain or branched aliphatic, alicyclic, aromatic or alkyl-
substituted aromatic hydrocarbons.
4 Interferences
Compounds of low polarity (e.g. halogenated hydrocarbons) and high concentrations of polar substances can
interfere with the determination.
Surface-active substances interfere with the extraction step.
5Principle
The water sample is extracted with an extracting agent. Polar substances are removed by clean-up on Florisil. The
purified aliquot is analysed by capillary chromatography using a non-polar column and a flame
...