EN 16161:2012

2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.Kakovost vode - Navodilo za uporabo absorpcijskih tehnik in vivo za oceno koncentracije klorofila a v morskih in celinskih vodahWasserbeschaffenheit - Anleitung für die Anwendung der in-vivo-Absorption zur Abschätzung des Chlorophyllgehaltes in Meer- und SüßwasserQualité de l'eau - Lignes directrices sur l'utilisation de techniques d'absorption in vivo pour l'estimation de la concentration de chlorophylle a dans des échantillons d'eau de mer et d'eau douceWater quality - Guidance on the use of in vivo absorption techniques for the estimation of chlorophyll-a concentration in marine and fresh water samples13.060.70Preiskava bioloških lastnosti vodeExamination of biological properties of water13.060.10Voda iz naravnih virovWater of natural resourcesICS:Ta slovenski standard je istoveten z:EN 16161:2012SIST EN 16161:2013en,fr,de01-januar-2013SIST EN 16161:2013SLOVENSKI
STANDARD
EUROPEAN STANDARD NORME EUROPÉENNE EUROPÄISCHE NORM
EN 16161
July 2012 ICS 13.060.70 English Version
Water quality - Guidance on the use of in vivo absorption techniques for the estimation of chlorophyll-a concentration in marine and fresh water samples
Qualité de l'eau - Lignes directrices sur l'utilisation des techniques d'absorption in vivo pour l'estimation de la concentration de chlorophylle-a dans les eaux douces et eaux marines
Wasserbeschaffenheit - Anleitung für die Anwendung der in-vivo-Absorption zur Abschätzung der Chlorophyll a-Konzentration in Meer- und Süßwasser This European Standard was approved by CEN on 17 May 2012.
CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management Centre has the same status as the official versions.
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Published in vivo specific chlorophyll a absorption spectrum . 11Annex B (informative)
Determination of the appropriate chlorophyll-a specific spectral absorption coefficient for the IVP system . 13Annex C (informative)
Factors influencing the chlorophyll-a estimation . 16C.1Breakdown pigments . 16C.2Package effect . 17C.3Chlorophyll-b . 18C.4Phycocyanin . 18Annex D (informative)
Examples of paired sample method validation  Comparison of extraction and in vivo methods under operational conditions . 20D.1General . 20D.2The IRH Laboratory Data Set . 20D.3The Adasa Sistemas Laboratory Data Set . 21Annex E (informative)
Validation of the spectrometric technique by determining the chlorophyll-a specific absorption of a set of algal samples . 22E.1Laboratory sample data for validation a*chl_sys the appropriate system unit chlorophyll-a response peak height . 22E.2In vivo photometric chlorophyll-a and the package effect . 24Bibliography . 25 SIST EN 16161:2013

chlorophyll-a concentration in marine and fresh waters. This European Standard is comprised of the following:  definition of the equipment requirement;  a priori data and mathematical tools;  recommendations for verification of measurement system performance and consideration of factors that can influence measurements;  listing of the procedures to be implemented. 2 Normative references The following documents, in whole or in part, are normatively referenced in this document and are indispensable for its application. For dated references, only the edition cited applies. For undated references, the latest edition of the referenced document (including any amendments) applies. ENV 13005, Guide to the expression of uncertainty in measurement 3 Terms and definitions For the purposes of this document, the following terms and definitions apply. 3.1 absorption coefficient
a
natural logarithm of the ratio between the light intensity entering and corresponding intensity emerging directly through a sample of water divided by the sample path length (in metres) in cases where the scattering of light is negligible Note 1 to entry: The unit is m-1.
Note 2 to entry: A spectrophotometer often gives the Log10 of the ratio in place of the natural logarithm. 3.2 extinction
c
sum of losses of directly transmitted light by absorption and scattering
Note 1 to entry: The unit is m-1.
Note 2 to entry: The extinction c is related to absorption a and scattering b, by bac+=. 3.3 extractive photometric
EP method of chlorophyll concentration estimation involving extraction and absorption measurement SIST EN 16161:2013

IVP method of assessing chlorophyll-a concentration through the use of in vivo spectral photometry 3.5 package effect flattening of a spectral absorption feature arising from excessive absorbing molecule concentration within cells 3.6 resolution width at half height of the instrument response function 3.7 scattering coefficient b
natural logarithm of the ratio between the light intensity entering and corresponding intensity emerging directly through a sample of water divided by the sample path length (in metres) in cases where the absorption of light is negligible Note 1 to entry: The unit is m-1.
3.8 spectrum set of data of a sample taken over a defined wavelength range and by a defined resolution
3.9 wavelength range range from minimum to maximum wavelength over which a spectrum is described 4 Principle The in vivo photometric absorption technique (IVP) is based on: a) the additive nature of absorption of individual constituents within a suspension; b) the use of a priori knowledge about the absorption features of chlorophyll-a in the wavelength area of approximately 675 nm; c) the absence of other components interfering with spectral features of chlorophyll-a in this region; d) the use of a measurement cell (a cuvette or other sample receptacle) of sufficient length and spectrophotometer of sufficient performance to enable the absorption feature of chlorophyll-a to be identified at the concentration levels required; e) the availability of a suitable algorithm to identify and quantify the distinctive chlorophyll-a absorption feature within a spectral absorption measurement. 5 Apparatus 5.1 Spectrophotometer, or equivalent, with the capability to determine the absorption spectrum of an in situ sample of water. The spectral measuring instrument shall be capable of measuring the absorption of the sample in a range between 600 nm and 750 nm with a resolution better than 10 nm. The data capture is carried out in measuring intervals of 5 nm or smaller. SIST EN 16161:2013
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