ASTM E1044-96(2001)

NOTICE: This standard has either been superseded and replaced by a new version or withdrawn.
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Designation: E 1044 – 96 (Reapproved 2001)
Standard Specification for
Glass Serological Pipets (General Purpose and Kahn)
This standard is issued under the fixed designation E 1044; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision. A number in parentheses indicates the year of last reapproval. A
superscript epsilon (e) indicates an editorial change since the last revision or reapproval.
1. Scope the 0.25 mL Kahn pipet; this Kahn pipet shall have a clear
ungraduated space of not less than 63 mm between the
1.1 This specification covers glass serological pipets, used
lowermost graduation mark and the tip of the pipet. The pipet
in measuring volumes of liquids.
shall be filled about 10 mm above the capacity line. Remove
2. Referenced Documents
any water on the outside of the tip by a downward wipe with
filter paper. Next, place the tip in contact with the wetted wall
2.1 ASTM Standards:
of a beaker and slowly lower the meniscus to the capacity line.
E 438 Specification for Glasses in Laboratory Apparatus
Do not remove any water remaining on the tip at this time.
E 542 Practice for Calibration of Volumetric Ware
Delivery of the contents into a receiving vessel is made with
E 671 Specification for Maximum Permissible Thermal Re-
the tip in contact with the wall of the vessel. When the water
sidual Stress in Annealed Glass Laboratory Apparatus
has ceased to flow, the water remaining in the tip is then blown
E 694 Specification for Volumetric Ware
out with one firm puff with the tip in contact with the wall of
E 920 Specification for Commercially Packaged Laboratory
the vessel, if possible. No after-drainage period is allowed.
Apparatus
Capacity tolerances shall be within the limits given in Table 1.
E 921 Specification for Export Packaged Laboratory Appa-
4.3 Annealing—The maximum residual thermal stress shall
ratus
be such as to conform to Specification E 671, except that
E 1133 Practice for PerformanceTesting of Packaged Labo-
tempered tips or tempered tops, or both, may be supplied at the
ratory Apparatus for United States Government Procure-
option of the manufacturer. The tempered tip, when examined
ments
in index oil that matches the refractive index of the glass being
E 1157 Specification for Sampling and Testing of Reusable
used, shall have a temper between 75 and 220 nm.
Laboratory Glassware
5. Design
3. Classification
5.1 Shape—The pipets shall be straight and of one-piece
3.1 Style and Capacity—Pipetscoveredbythisspecification
construction. Any cross section of a pipet taken in a plane
shall be of the following styles and capacities:
perpendicular to the longitudinal axis shall be circular.
3.1.1 Style I—General purpose top end.
5.2 Delivery Tips—Delivery tips shall be made with a
3.1.2 Style II—Cotton plugging top end.
gradual taper of 15 to 30 mm for capacities up to 5 mL
3.1.3 Style III—Cotton plugging top end, large tip opening.
inclusive, and 20 to 35 mm for 10 and 25 mL capacity pipets.
3.1.4 Capacities (mL)—0.1; 0.1 (Kahn); 0.2; 0.2 (Kahn);
The end of the tip shall be perpendicular to the longitudinal
0.25 (Kahn); 0.5; 0.60 (Kahn); 1.0; 2.0; 5.0; 10.0; and 25.0.
axis of the pipet. Sudden constriction at the orifice shall not be
4. General Requirements
acceptable. The outside edge of the tip shall be bevelled
slightly at the end, and the bevel shall be ground or firepol-
4.1 Borosilicate Glass—Borosilicate glass for pipets shall
ished.
conform to the glass requirements of Type 1, Class A or B of
5.3 Zero Graduation Line Position—The distance from the
Specification E 438.
top end to the top graduation of all pipets (except for 0.5 mL
4.2 Calibration—Pipets shall be calibrated in accordance
and Kahn pipets) shall be not less than 100 mm nor more than
with Practice E 542 to deliver (TD) the intended capacity of
150 mm from the zero graduation line; for 0.5 mL and Kahn
distilled water at 20°C when the last drop is blown out, except
pipets this distance shall be not less than 90 mm nor more than
150 mm.
This specification is under the jurisdiction of ASTM Committee E41 on
5.4 Dimensions and Outflow Times—The limiting dimen-
Laboratory Apparatus and is the direct responsibility of Subcommittee E41.01 on
sions and outflow times shall be as shown in Table 1. Outflow
Apparatus.
times shall be determined on unplugged pipets using distilled
Current edition approved April 10, 1996. Published June 1996. Originally
published as E 1044 – 85.
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