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ASTM D7391-09

(Test Method)

Standard Test Method for Categorization and Quantification of Airborne Fungal Structures in an Inertial Impaction Sample by Optical Microscopy

Standard Test Method for Categorization and Quantification of Airborne Fungal Structures in an Inertial Impaction Sample by Optical Microscopy

SIGNIFICANCE AND USE
This test method is used to estimate and categorize the number and type of fungal structures present on an inertial impactor sample.
Fungal structures are identified and quantified regardless of whether they would or would not grow in culture.
It must be emphasized that the detector in this method is the analyst, and therefore results are subjective, depending on the experience, training, qualification, and mental and optical fatigue of the analyst.
SCOPE
1.1 This test method is a procedure that uses direct microscopy to analyze the deposit on an inertial impaction sample.
1.2 This test method describes procedures for categorizing and enumerating fungal structures by morphological type. Typically, categories may be as small as genus (for example, Cladosporium) or as large as phylum (for example, basidiospores).
1.3 This method contains two procedures for enumerating fungal structures: one for slit impaction samples and one for circular impaction samples. This test method is applicable for impaction air samples, for which a known volume of air (at a rate as recommended by the manufacturer) has been drawn, and is also applicable for blank impaction samples.
1.4 Enumeration results are presented in fungal structures/sample (fs/sample) and fungal structures/m3 (fs/m3).
1.5 The range of enumeration results that can be determined with this method depends on the size of the spores on the sample trace, the amount of particulate matter on the sample trace, the percentage of the sample trace counted, and the volume of air sampled.
1.6 This method addresses only the analysis of samples. The sampling process and interpretation of results is outside the scope of this method.
1.7 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.
1.8 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

General Information

Status
Historical
Publication Date
30-Apr-2009
ICS
07.100.99 - Other standards related to microbiology
Technical Committee
D22 - Air Quality
Drafting Committee
D22.08 - Assessment, Sampling, and Analysis of Microorganisms
Current Stage
Ref Project

Relations

Replaced By
ASTM D7391-17 - Standard Test Method for Categorization and Quantification of Airborne Fungal Structures in an Inertial Impaction Sample by Optical Microscopy
Effective Date
15-Mar-2017
Referred By
ASTM D1356-20a - Standard Terminology Relating to Sampling and Analysis of Atmospheres
Effective Date
01-May-2009
Referred By
ASTM D7788-14(2023) - Standard Practice for Collection of Total Airborne Fungal Structures via Inertial Impaction Methodology
Effective Date
01-May-2009
Referred By
ASTM D8219-19 - Standard Guide for Cleaning and Disinfection at a Cannabis Cultivation Center
Effective Date
01-May-2009

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ASTM D7391-09 - Standard Test Method for Categorization and Quantification of Airborne Fungal Structures in an Inertial Impaction Sample by Optical MicroscopyASTM D7391-09 - Standard Test Method for Categorization and Quantification of Airborne Fungal Structures in an Inertial Impaction Sample by Optical Microscopy
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ASTM D7391-09 - Standard Test Method for Categorization and Quantification of Airborne Fungal Structures in an Inertial Impaction Sample by Optical Microscopy
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Standards Content (Sample)

NOTICE: This standard has either been superseded and replaced by a new version or withdrawn.
Contact ASTM International (www.astm.org) for the latest information
Designation:D7391 −09
Standard Test Method for
Categorization and Quantification of Airborne Fungal
Structures in an Inertial Impaction Sample by Optical
1
Microscopy
This standard is issued under the fixed designation D7391; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision.Anumber in parentheses indicates the year of last reapproval.A
superscript epsilon (´) indicates an editorial change since the last revision or reapproval.
1. Scope 2. Referenced Documents
2
1.1 This test method is a procedure that uses direct micros- 2.1 ASTM Standards:
copy to analyze the deposit on an inertial impaction sample. D1193Specification for Reagent Water
1.2 This test method describes procedures for categorizing
3. Terminology
and enumerating fungal structures by morphological type.
Typically, categories may be as small as genus (for example, 3.1 ASTM Definitions (see ASTM Terminology Dictionary):
Cladosporium) or as large as phylum (for example, basidi- 3.1.1 numerical aperture
ospores).
3.2 Definitions of Terms Specific to This Standard:
1.3 This method contains two procedures for enumerating 3.2.1 circular impaction sample, n—a sample of airborne
particulate matter collected via a device that draws air through
fungal structures: one for slit impaction samples and one for
circular impaction samples. This test method is applicable for a round aperture at a specified rate, impacting the particles
impaction air samples, for which a known volume of air (at a suspended in the air onto an adhesive medium, resulting in a
rate as recommended by the manufacturer) has been drawn, circularareaofdeposition.Acircularimpactionsamplemaybe
and is also applicable for blank impaction samples. collectedbymeansofacassettemanufacturedforthatpurpose,
or by means of a sampling device that requires slides to be
1.4 Enumeration results are presented in fungal structures/
pre-coated with impaction medium.
3 3
sample (fs/sample) and fungal structures/m (fs/m ).
3.2.2 debris rating, n—a distinct value assigned to an
1.5 Therangeofenumerationresultsthatcanbedetermined
impactor sample based on the percentage of the sample area
with this method depends on the size of the spores on the
potentially obscured by particulate matter, and ranging from 0
sample trace, the amount of particulate matter on the sample
to 5.
trace, the percentage of the sample trace counted, and the
3.2.3 field blank, n—asampleslideorcassettecarriedtothe
volume of air sampled.
sampling site, exposed to sampling conditions (for example,
1.6 Thismethodaddressesonlytheanalysisofsamples.The
seals opened), returned to the laboratory, treated as a sample,
sampling process and interpretation of results is outside the
and carried through all steps of the analysis.
scope of this method.
3.2.4 hyaline, adj—colorless.
1.7 The values stated in SI units are to be regarded as
3.2.5 impaction medium, n—asubstanceappliedtoamicro-
standard. No other units of measurement are included in this
scope slide used to collect (or capture) particulate matter
standard.
during sampling.
1.8 This standard does not purport to address all of the
3.2.6 impaction sample, n—asampletakenusingimpaction,
safety concerns, if any, associated with its use. It is the
for example, slit impaction sample, circular impaction sample.
responsibility of the user of this standard to establish appro-
3.2.7 inertial impactor, n—a device for collecting particles
priate safety and health practices and determine the applica-
separated from an air stream by inertia to force an impact onto
bility of regulatory limitations prior to use.
an adhesive surface. Inertial impactors are available in many
1
This test method is under the jurisdiction of ASTM Committee D22 on Air
2
Quality and is the direct responsibility of Subcommittee D22.08 on Sampling and For referenced ASTM standards, visit the ASTM website, www.astm.org, or
Analysis of Mold. contact ASTM Customer Service at service@astm.org. For Annual Book of ASTM
Current edition approved May 1, 2009. Published June 2009. DOI: 10.1520/ Standards volume information, refer to the standard’s Document Summary page on
D7391-09. the ASTM website.
Copyright © ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959. United States
1

---------------------- Page: 1 ----------------------
D7391−09
designs,includingthosehavingaslitjet,yieldingarectangular ommended sample flow rate. Each sample consists of an
sample trace, and a circular jet, yielding a circular sample optically clear substrate coated with an adhesive and optically
trace. transparent medium onto which particles have been deposited
through inertial impaction.
3.2.8 magnification/resolution combination 1, n—~150-
400× total magnification and a point to point re
...

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