ASTM E2754-10

NOTICE: This standard has either been superseded and replaced by a new version or withdrawn.
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Designation:E2754 −10
Standard Specification for
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Delphastus catalinae (Horn) (Coleoptera:Coccinellidae)
This standard is issued under the fixed designation E2754; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision.Anumber in parentheses indicates the year of last reapproval.A
superscript epsilon (´) indicates an editorial change since the last revision or reapproval.
1. Scope 4.6 Species—catalinae.
1.1 This specification covers information on and the test
5. Physical Properties
method for determining the purity, sex ratio, and number of
live adults present in commercial containers of Delphastus
5.1 Number—Shipments of D. catalinae must have a mini-
catalinae (Horn), a predator of whitefly.
mum number of live adult beetles as specified on container or
1.2 The values stated in SI units are to be regarded as accompanying literature.
standard. No other units of measurement are included in this
5.2 Sex Ratio—D. catalinae has a male:female sex ratio of
standard.
1:1.
2. Referenced Documents
5.3 Sex Identification—Color of both sexes is mostly
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2.1 ASTM Standards: reddish-brown, male head and legs yellow; female similar to
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E2200Specification for Information Included with Packag- male except head reddish-yellow. See Gordon for a more
ing of Multi-Cellular Biological Control Organisms detailed description.
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(Withdrawn 2010)
6. Summary of Test Method (Determining the Viability,
3. Terminology
Purity, Sex Ratio, and Numbers of Living Adults in
3.1 Definitions of Terms Specific to This Standard: Containers of D. catalinae)
3.1.1 viability—qualitative assessment of activity of beetles
6.1 The number of live beetles, sex ratio, and presence of
after warming.
other live insects or mites will be determined by counting and
3.1.2 life stage when shipped—adult.
examining individual containers from shipments of D. catali-
3.1.3 name of product—Delphastus catalinae (Horn). nae.
3.1.4 preferred host prey—most species of whitefly
7. Significance and Use
including, Trialeurodes vaporariorum, Bemisia tabaci.
7.1 The biological control of whitefly with D. catalinae
4. Classification
depends on accurate release rates of predators. Accurate
4.1 Phylum—Arthropoda.
packaging, and maintenance of purity, sex ratio, and viability
4.2 Class—Insecta. of shipments is critical to this success. This test will be
typically employed by producers and users of the product to
4.3 Order—Coleoptera.
assess the number of live biological control agents delivered.
4.4 Family—Coccinellidae.
4.5 Genus—Delphastus.
8. Materials
8.1 Dissecting microscope or magnifier (10×).
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This specification is under the jurisdiction of ASTM Committee E35 on
8.2 Fine (#1) paint brush or forceps.
Pesticides and Alternative Control Agents and is the direct responsibility of
Subcommittee E35.30 on Natural Multi-Cellular (Metazoan) Biological Control
8.3 Funnel.
Organisms.
Current edition approved April 1, 2010. Published May 2010. DOI: 10.1520/
8.4 Holding container.
E2754–10.
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For referenced ASTM standards, visit the ASTM website, www.astm.org, or
contact ASTM Customer Service at service@astm.org. For Annual Book of ASTM
Standards volume information, refer to the standard’s Document Summary page on
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the ASTM website. Gordon, R.D., SouthAmerican Coccinellidae (Coleoptera) Part III:Taxonomic
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The last approved version of this historical standard is referenced on Revision of the Western Hemisphere Genus Delphastus Casey, Frustula entomol.,
www.astm.org. Vol 17, No. 3, 1994, pp. 100–101.
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E2754−10
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8.5 Aspirator (Mod. 1135A). beetles, remove any packing materials and identify and record
the presence of any live arthropods other than adult D.
9. Test Unit
catalinae beetles. Record live and dead beetles. Retain the live
9.1 A single container of D. catalinae is considered a test beetlesifthesexratioisrequired.Inmultiplebottleshipments,
check three bottles.
unit. In large shipments, a minimum of three containers per
shipment should be chosen randomly.
13. Sex Ratio
10. Pre-Test Conditions
13.1 Normal sex ratio is 50% female.
10.1 If samples must be held before testing, hold containers
13.2 Count males and females using handling technique
with D. catalinae out of direct sunlight between 10 and 12°C
above. The sex is determined by the color of the head: males
for a maximum of 24 h prior to testing.
have a yellow head and yellow legs, females have a reddish-
yellow head. The head is most easily seen on live beetles as it
11. Sample Preparation and Treatment 4
is extended when they are walking. See Gordon for a more
11.1 Cool samples to 5 to 10°C for 1 to 2 h prior to
...