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ISO

ISO/TC 34/SC 9/WG 25 - Whole-genome sequencing for typing and genomic characterization

Séquençage complet du génome pour le typage et la caractérisation génomique

General Information

Status
Active
Work Field
Information technology
Parent
ISO/TC 34/SC 9 - Microbiology

Frequently Asked Questions

ISO/TC 34/SC 9/WG 25 is a Subcommittee within the International Organization for Standardization (ISO). It is named "Whole-genome sequencing for typing and genomic characterization". This committee has published 1 standards.

ISO/TC 34/SC 9/WG 25 develops ISO standards in the area of Information technology. Currently, there are 1 published standards from this subcommittee.

The International Organization for Standardization (ISO) is an independent, non-governmental international organization that develops and publishes international standards. Founded in 1947 and headquartered in Geneva, Switzerland, ISO brings together experts from 170+ member countries to share knowledge and develop voluntary, consensus-based standards that support innovation and provide solutions to global challenges.

A Subcommittee (SC) in ISO operates under a Technical Committee and focuses on a specific subset of the TC's scope. Subcommittees develop standards and technical specifications in their specialized area, reporting to their parent Technical Committee. They may also have working groups for detailed technical work.

Standardization Organization
ICS
Directive
Mandate
50
ISO
ISO 23418:2022(Main)
Microbiology of the food chain - Whole genome sequencing for typing and genomic characterization of bacteria - General requirements and guidance

This document specifies the minimum requirements for generating and analysing whole genome sequencing (WGS) data of bacteria obtained from the food chain. This process can include the following stages: a) handling of bacterial cultures; b) axenic genomic DNA isolation; c) library preparation, sequencing, and assessment of raw DNA sequence read quality and storage; d) bioinformatics analysis for determining genetic relatedness, genetic content and predicting phenotype, and bioinformatics pipeline validation; e) metadata capture and sequence repository deposition; f) validation of the end-to-end WGS workflow (fit for purpose for intended application). This document is applicable to bacteria isolated from: - products intended for human consumption; - products intended for animal feed; - environmental samples from food and feed handling and production areas; - samples from the primary production stage.

  • Standard
    45 pages
    English language
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  • Standard
    50 pages
    French language
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