SIST EN ISO 11212-2:1998
(Main)Starch and derived products - Heavy metals content - Part 2: Determination of mercury content by atomic absorption spectrometry (ISO 11212-2:1997)
Starch and derived products - Heavy metals content - Part 2: Determination of mercury content by atomic absorption spectrometry (ISO 11212-2:1997)
Stärke und Stärkederivate - Schwermetallgehalt - Teil 2: Bestimmung des Quecksilbergehaltes durch Atomabsorptionsspektrometrie (ISO 11212-2:1997)
Dieser Teil von ISO 11212 legt ein Verfahren für die Bestimmung des Quecksilbergehaltes von Stärke, einschließlich von Derivaten und Nebenprodukten, durch Atomabsorptionsspektrometrie mit Kaltdampferzeugung fest. Die gegenwärtig verfügbaren Kaltdampferzeuger verwenden sehr verschiedene Verfahren; es ist folglich unmöglich, ein umfassendes Verfahren vorzuschlagen, das das Erreichen von zufriedenstellenden Ergebnissen für alle Gerätetypen sicherstellt.
Amidons, fécules et produits dérivés - Teneur en métaux lourds - Partie 2: Détermination de la teneur en mercure par spectrométrie d'absorption atomique (ISO 11212-2:1997)
La présente partie de l'ISO 11212 prescrit une méthode pour la détermination de la teneur en mercure dans les amidons, fécules, produits dérivés et sous-produits d'amidonnerie par spectrométrie d'absorption atomique avec génération de vapeur froide. Les appareils générateurs de vapeur froide actuellement commercialisés utilisant des techniques très différentes, il est donc impossible de proposer une méthode détaillée susceptible de garantir l'obtention de résultats satisfaisants sur tous les types d'appareils. Il convient donc que chaque analyste procède à l'optimisation des conditions d'utilisation de son propre instrument sur la base des recommandations générales ou particulières qui lui sont données.
Škrob in derivati škroba - Delež težkih kovin - 2. del: Določevanje živega srebra z atomsko absorpcijsko spektrometrijo (ISO 11212-2:1997)
General Information
- Status
- Published
- Publication Date
- 31-Oct-1998
- Technical Committee
- KŽP - Agricultural food products
- Current Stage
- 6060 - National Implementation/Publication (Adopted Project)
- Start Date
- 01-Nov-1998
- Due Date
- 01-Nov-1998
- Completion Date
- 01-Nov-1998
Relations
- Effective Date
- 22-Dec-2008
Overview
SIST EN ISO 11212-2:1998 is an international standard developed by CEN that specifies a precise analytical method for determining the mercury content in starch and its derived products. Mercury, a toxic heavy metal, can contaminate starch during processing or from the environment, posing health risks. This standard outlines the use of atomic absorption spectrometry with cold-vapour generation to accurately quantify mercury levels in starch materials. This method supports quality control, regulatory compliance, and safety assurance in starch-related industries.
Key Topics
Scope and Purpose
Defines a standardized procedure to measure mercury content (expressed in micrograms per kilogram) in starch, derivatives, and by-products using atomic absorption spectrometry.Analytical Principle
Involves wet digestion of samples, reduction of mercury ions to metallic mercury through sodium borohydride or tin(II) chloride in hydrochloric acid, and detection of monoatomic mercury vapour absorbance at 253.7 nm wavelength.Sample Preparation and Digestion
Homogenization of starch samples followed by reflux distillation with nitric acid and hydrogen peroxide for organic matrix breakdown.Reagents and Calibration
Use of purified reagents (nitric acid, hydrogen peroxide, sodium borohydride, tin(II) chloride, hydrochloric acid) and preparation of mercury standard solutions for calibration curves.Apparatus Specifications
Requires a digestion apparatus with Soxhlet extraction tube and cooling system, atomic absorption spectrometer with mercury discharge lamp, and a cold mercury vapour generator equipped with an automatic sampling device.Measurement and Calculation
Absorbance measurements are compared against a calibration curve to calculate mercury concentration. The standard includes formulas and guidance to express results accurately.Precision and Repeatability
Interlaboratory studies confirm the method’s reliability with defined repeatability and reproducibility limits, ensuring consistent mercury detection across different labs and testing conditions.Safety Considerations
Emphasizes the toxicity and volatility of mercury, recommending precautions such as verification of apparatus tightness and controlled digestion temperature to prevent mercury vapour loss.
Applications
Food Safety Testing
Ensures that starch ingredients used in food manufacturing comply with mercury contamination limits, protecting consumer health.Quality Control in Starch Production
Enables manufacturers to monitor and minimize heavy metal impurities, maintaining product quality and meeting regulatory standards.Environmental Monitoring
Assists laboratories in detecting mercury contamination in starch derived from agricultural sources exposed to polluted environments.Regulatory Compliance
Provides a validated standard method for reporting mercury content in starch products, aligning with food safety and chemical contaminant regulations.
Related Standards
EN ISO 11212 is a multi-part standard series addressing heavy metals in starch products, including:
- EN ISO 11212-1: Determination of arsenic content by atomic absorption spectrometry.
- EN ISO 11212-3: Determination of lead content by atomic absorption spectrometry with electrothermal atomization.
- EN ISO 11212-4: Determination of cadmium content by atomic absorption spectrometry with electrothermal atomization.
These related standards collectively support comprehensive heavy metal analysis to ensure starch product safety and compliance.
Keywords: EN ISO 11212-2, mercury determination, starch heavy metals, atomic absorption spectrometry, cold-vapour generation, mercury content analysis, starch quality control, heavy metal testing, food safety standards, mercury spectrometry method.
Frequently Asked Questions
SIST EN ISO 11212-2:1998 is a standard published by the Slovenian Institute for Standardization (SIST). Its full title is "Starch and derived products - Heavy metals content - Part 2: Determination of mercury content by atomic absorption spectrometry (ISO 11212-2:1997)". This standard covers: La présente partie de l'ISO 11212 prescrit une méthode pour la détermination de la teneur en mercure dans les amidons, fécules, produits dérivés et sous-produits d'amidonnerie par spectrométrie d'absorption atomique avec génération de vapeur froide. Les appareils générateurs de vapeur froide actuellement commercialisés utilisant des techniques très différentes, il est donc impossible de proposer une méthode détaillée susceptible de garantir l'obtention de résultats satisfaisants sur tous les types d'appareils. Il convient donc que chaque analyste procède à l'optimisation des conditions d'utilisation de son propre instrument sur la base des recommandations générales ou particulières qui lui sont données.
La présente partie de l'ISO 11212 prescrit une méthode pour la détermination de la teneur en mercure dans les amidons, fécules, produits dérivés et sous-produits d'amidonnerie par spectrométrie d'absorption atomique avec génération de vapeur froide. Les appareils générateurs de vapeur froide actuellement commercialisés utilisant des techniques très différentes, il est donc impossible de proposer une méthode détaillée susceptible de garantir l'obtention de résultats satisfaisants sur tous les types d'appareils. Il convient donc que chaque analyste procède à l'optimisation des conditions d'utilisation de son propre instrument sur la base des recommandations générales ou particulières qui lui sont données.
SIST EN ISO 11212-2:1998 is classified under the following ICS (International Classification for Standards) categories: 67.180.20 - Starch and derived products. The ICS classification helps identify the subject area and facilitates finding related standards.
SIST EN ISO 11212-2:1998 has the following relationships with other standards: It is excused to SIST EN ISO 11212-1:1998. Understanding these relationships helps ensure you are using the most current and applicable version of the standard.
You can purchase SIST EN ISO 11212-2:1998 directly from iTeh Standards. The document is available in PDF format and is delivered instantly after payment. Add the standard to your cart and complete the secure checkout process. iTeh Standards is an authorized distributor of SIST standards.
Standards Content (Sample)
SLOVENSKI STANDARD
01-november-1998
âNURELQGHULYDWLãNURED'HOHåWHåNLKNRYLQGHO 'RORþHYDQMHåLYHJDVUHEUD]
DWRPVNRDEVRUSFLMVNRVSHNWURPHWULMR ,62
Starch and derived products - Heavy metals content - Part 2: Determination of mercury
content by atomic absorption spectrometry (ISO 11212-2:1997)
Stärke und Stärkederivate - Schwermetallgehalt - Teil 2: Bestimmung des
Quecksilbergehaltes durch Atomabsorptionsspektrometrie (ISO 11212-2:1997)
Amidons, fécules et produits dérivés - Teneur en métaux lourds - Partie 2: Détermination
de la teneur en mercure par spectrométrie d'absorption atomique (ISO 11212-2:1997)
Ta slovenski standard je istoveten z: EN ISO 11212-2:1997
ICS:
67.180.20 Škrob in izdelki iz njega Starch and derived products
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.
INTERNATIONAL Is0
11212-2
STANDARD
First edition
1997003- 15
Starch and derived products - Heavy
metals content -
Part 2:
Determination of mercury content by atomic
absorption spectrometry
Amidons, f&u/es et produits d&iv& - Teneur en mbtaux lourds -
Parfie 2: Dbtermination de la teneur en mercure par spectrom&rie
d ’absorption atomique
Reference number
IS0 11212-2:1997(E)
IS0 11212-2: 1997(E)
Foreword
IS0 (the International Organization for Standardization) is a worldwide
federation of national standards bodies (IS0 member bodies). The work of
preparing international Standards is normally carried out through IS0
technical committees. Each member body interested in a subject for which
a technical committee has been established has the right to be represented
on that committee. International organizations, governmental and non-
governmental, in liaison with ISO, also take part in the work. IS0
collaborates closely with the International Electrotechnical Commission
(IEC) on all matters of electrotechnical standardization.
Draft International Standards adopted by the technical committees are
circulated to the member bodies for voting. Publication as an International
Standard requires approval by at least 75 % of the member bodies casting
a vote.
International Standard IS0 11212-2 was prepared by Technical Committee
ISOKC 93, Starch (including derivatives and by-products).
IS0 11212 consists of the following parts, under the general title Starch
and derived products - Heavy metals content.
- Part 1: Determination of arsenic content by atomic absorption
spectrometry
- Part 2: Determination of mercury content by atomic absorption
spectrometry
- Part 3: Determination of lead content by atomic absorption
spectrometry with electrothermal atomization
- Part 4: Determination of cadmium content by atomic absorption
spectrometry with electrothermal atomization
Annex A of this part of IS0 11212 is for information only.
0 IS0 1997
All rights reserved. Unless otherwise specified, no part of this publication may be reproduced
or utilized in any form or by any means, electronic or mechanical, including photocopying and
microfilm, without permission in writing from the publisher.
International Organization for Standardization
Case postale 56 l CH-1211 Geneve 20 l Switzerland
Internet
central @ iso.ch
x.400 c=ch; a=400net; p=iso; o=isocs; s=central
Printed in Switzerland
ii
INTERNATIONAL STANDARD o 1s0 IS0 11212=2:1997(E)
Starch and derived products - Heavy metals content -
Part 2:
Determination of mercury content by atomic absorption spectrometry
1 Scope
This part of IS0 11212 specifies a method for the determination of the mercury content of starch, including
derivatives and by-products, by atomic absorption spectrometry with cold-vapour generation.
The cold-vapour generators currently available use very different techniques; it is thus impossible to propose a
comprehensive method likely to ensure the attainment of satisfactory results on all types of apparatus. Each analyst
should therefore optimize the conditions of use of his/her own apparatus on the basis of general or particular
instructions.
2 Definition
For the purposes of this part of IS0 11212, the following definition applies.
2.1 mercury content: Quantity of mercury determined in accordance with the conditions specified in this method
and expressed as mercury (Hg), in micrograms per kilogram of the product as received.
3 Principle
Wet digestion of the organic matrix. Reduction of mercury (Hg2+) to metallic mercury by hydrogen resulting from the
action of sodium borohydride (or tin(ll) chloride) on hydrochloric acid. Entrainment of the mercury vapour by a flow
of gas and determination of monoatomic mercury vapour by atomic absorption spectrometry in a quartz cell.
Measurement of the absorbance at a wavelength of 253,7 nm.
Determination of the concentration of mercury in the sample by means of a calibration curve.
4 Reagents
Use only reagents of recognized analytical grade and distilled water or water of equivalent purity.
4.1 Nitric acid (p20 = I,38 g/ml).
4.2 Hydrogen peroxide, 30 % (WV) solution.
0 IS0
IS0 11212=2:1997(E)
4.3 Sodium borohydride solution
Prepare a solution at the concentration recommended in the instructions for use of the cold-vapour generator (5.3).
4.4 Tin(N) chloride solution
Prepare a solution at the concentration recommended in the instructions for use of the cold-vapour generator (5.3).
4.5 Hydrochloric acid solution
Prepare a solution at the concentration recommended in the instructions for use of the cold-vapour generator (5.3).
4.6 Mercury standard solution, 1 g/l.
Standard solutions are commercially available at this concentration. These solutions may be prepared by weighing
and dissolving the salt or metal of known purity.
4.7 Calibration solutions
Before each series of measurements, prepare from the standard mercury solution (4.6) at least five calibration
solutions covering the range of concentrations to be determined. 100 ml of each calibration solution shall contain
7,5 ml of nitric acid (4.1).
5 Apparatus
All the glassware used shall be previously washed by means of suitable products (such as nitric acid) and rinsed
with distilled water to eliminate any trace of mercury.
Use ordinary laboratory apparatus and, in particular, the following.
5.1 Digestion apparatus (see figure l), made of borosilicate glass and consisting of three elements terminating
with conical ground joints (5.1 .l to 5.1.3).
5.1.1 Soxhlet extraction tube, of capacity 200 ml, equipped with a stopcock and a lateral tube connected directly
to the flask (5.1.3).
5.1.2 Cooling apparatus, 35 cm long, connected to the top of the Soxhlet extraction tube (5.1 .I).
5.1.3 Round-bot
...
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