SIST EN ISO 6888-2:1999

SLOVENSKI STANDARD
SIST EN ISO 6888-2:1999
01-december-1999
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Microbiology of food and animal feeding stuffs - Horizontal method for the enumeration of
coagulase-positive staphylococci (Staphylococcus aureus and other species) - Part 2:
Technique using rabbit plasma fibrinogen agar medium (ISO 6888-2:1999)
Mikrobiologie von Lebensmitteln und Futtermitteln - Horizontales Verfahren für die
Zählung von koagulase-positiven Staphylokokken (Staphylococcus aureus und andere
Spezies) - Teil 2: Verfahren mit Kaninchenplasma/Fibrinogen-Agar (ISO 6888-2:1999)
Microbiologie des aliments - Méthode horizontale pour le dénombrement des
staphylocoques a coagulase positive (Staphylococcus aureus et autres especes) - Partie
2: Technique utilisant le milieu gélosé au plasma de lapin et au fibrinogene (ISO 6888-
2:1999)
Ta slovenski standard je istoveten z: EN ISO 6888-2:1999
ICS:
07.100.30 Mikrobiologija živil Food microbiology
SIST EN ISO 6888-2:1999 en
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

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SIST EN ISO 6888-2:1999

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SIST EN ISO 6888-2:1999

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SIST EN ISO 6888-2:1999

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SIST EN ISO 6888-2:1999
INTERNATIONAL ISO
STANDARD 6888-2
First edition
1999-02-15
Microbiology of food and animal feeding
stuffs — Horizontal method for the
enumeration of coagulase-positive
staphylococci (Staphylococcus aureus
and other species) —
Part 2:
Technique using rabbit plasma fibrinogen agar
medium
Microbiologie des aliments — Méthode horizontale pour le dénombrement
des staphylocoques à coagulase positive (Staphylococcus aureus et autres
espèces) —
Partie 2: Technique utilisant le milieu gélosé au plasma de lapin et au
fibrinogène
A
Reference number
ISO 6888-2:1999(E)

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SIST EN ISO 6888-2:1999
ISO 6888-2:1999(E)
Contents
1 Scope .1
2 Normative references .1
3 Terms and definitions .1
4 Principle.2
5 Diluent and culture media.2
6 Apparatus and glassware .3
7 Sampling.4
8 Preparation of test sample.4
9 Procedure .4
10 Expression of results .5
11 Precision.6
12 Test report .6
Bibliography.7
©  ISO 1999
All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized in any form or by any means, electronic
or mechanical, including photocopying and microfilm, without permission in writing from the publisher.
International Organization for Standardization
Case postale 56 • CH-1211 Genève 20 • Switzerland
Internet iso@iso.ch
Printed in Switzerland
ii

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SIST EN ISO 6888-2:1999
© ISO
ISO 6888-2:1999(E)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards bodies (ISO
member bodies). The work of preparing International Standards is normally carried out through ISO technical
committees. Each member body interested in a subject for which a technical committee has been established has
the right to be represented on that committee. International organizations, governmental and non-governmental, in
liaison with ISO, also take part in the work. ISO collaborates closely with the International Electrotechnical
Commission (IEC) on all matters of electrotechnical standardization.
International Standards are drafted in accordance with the rules given in the ISO/IEC Directives, Part 3.
Draft International Standards adopted by the technical committees are circulated to the member bodies for voting.
Publication as an International Standard requires approval by at least 75 % of the member bodies casting a vote.
International Standard ISO 6888-1 was prepared by Technical Committee ISO/TC 34, Agricultural food products,
Subcommittee SC 9, Microbiology.
This first edition of ISO 6888-2, together with ISO 6888-1, cancels and replaces ISO 6888:1983, which has been
technically revised.
ISO 6888 consists of the following parts, under the general title Microbiology of food and animal feeding stuffs —
Horizontal method for the enumeration of coagulase-positive staphylococci (Staphylococcus aureus and other
species):
 Part 1: Technique using Baird-Parker agar medium
 Part 2: Technique using rabbit plasma fibrinogen agar medium
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SIST EN ISO 6888-2:1999
© ISO
ISO 6888-2:1999(E)
0  Introduction
0.1  Because of the large variety of food and feed products, this horizontal method may not be appropriate in every
detail for certain products. In this case, different methods, which are specific to these products may be used if
absolutely necessary for justified technical reasons. Nevertheless, every attempt should be made to apply this
horizontal method as far as possible.
When this part of ISO 6888 is next reviewed, account will be taken of all information then available regarding the
extent to which this horizontal method has been followed and the reasons for deviations from this method in the
case of particular products.
The harmonization of test methods cannot be immediate, and for certain groups of products International Standards
and/or national standards may already exist that do not comply with this horizontal method. It is hoped that when
such standards are reviewed they will be changed to comply with this part of ISO 6888 so that eventually the only
remaining departures from this horizontal method will be those necessary for well-established technical reasons.
0.2  ISO 6888 describes two horizontal methods (part 1 and part 2) for the enumeration of coagulase-positive
staphylococci among which enterotoxinogenic strains are encountered. It is mainly concerned with Staphylococcus
aureus, but also with S. intermedius and certain strains of S. hyicus.
In the general case, use part 1 of ISO 6888. However, it is preferable to use the procedure described in part 2 only
for foodstuffs (such as cheeses made from raw milk and certain raw meat products) likely to be contaminated by:
 staphylococci forming atypical colonies on a Baird-Parker agar medium;
background flora which can obscure the colonies being sought.

0.3  For the purposes of this part of ISO 6888, the characterization of staphylococci is based on a positive-
coagulase reaction, but it is recognized that some strains of Staphylococcus aureus give weakly positive coagulase
reactions. These latter strains may be confused with other bacteria but they may be distinguished from such other
bacteria by the use of additional tests not included in this part of ISO 6888, such as the sensitivity to lysostaphin, the
production of haemolysin, of thermostable nuclease and of acid from mannitol (see reference [1]).
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SIST EN ISO 6888-2:1999
INTERNATIONAL STANDARD  © ISO ISO 6888-2:1999(E)
Microbiology of food and animal feeding stuffs — Horizontal
method for the enumeration of coagulase-positive staphylococci
(Staphylococcus aureus and other species) —
Part 2:
Technique using rabbit plasma fibrinogen agar medium
1 Scope
This part of ISO 6888 describes a horizontal method for the enumeration of coagulase-positive staphylococci in
products intended for human consumption or feeding of animals by counting of colonies obtained on a solid medium
(rabbit plasma fibrinogen medium) after aerobic incubation at 35 °C or 37 °C (see reference [2]).
2 Normative references
The following normative documents contain provisions which, through reference in this text, constitute provisions of
this part of ISO 6888. For dated references, subsequent amendments to, or revisions of, any of these publications
do not apply. However, parties to agreements based on this part of ISO 6888 are encouraged to investigate the
possibility of applying the most recent editions of the normative documents indicated below. For undated
references, the latest edition of the normative document referred to applies. Members of ISO and IEC maintain
registers of currently valid International Standards.
ISO 6887-1, Microbiology of food and animal feeding stuffs — Rules for the preparation of the test samples, of initial
suspension and of decimal dilutions for microbiological examination — Part 1: General rules for the preparation of
the initial suspension and of decimal dilutions.
ISO 6888-1:1999, Microbiology of food and animal feeding stuffs — Horizontal method for the enumeration of
coagulase positive staphylococci (Staphylococcus aureus and other species) — Part 1: Technique using
Baird-Parker agar medium.
ISO 7218, Microbiology of food and animal feeding stuffs — General rules for microbiological examinations.
3 Terms and definitions
For the purposes of this part of ISO 6888, the following terms and definitions apply.
3.1
coagulase-positive staphylococci
bacteria which form typical colonies in a rabbit plasma fibrinogen selective agar medium when the test is carried out
according to the method specified in this part of ISO 6888
3.2
enumeration of the coagulase-p
...