SIST EN 14122:2003/AC:2006

SLOVENSKI STANDARD
SIST EN 14122:2003/AC:2006
01-marec-2006
äLYLOD±'RORþHYDQMHYLWDPLQD%V+3/&
Foodstuffs - Determination of vitamin B1 by HPLC
Lebensmittel - Bestimmung von Vitamin B1 mit HPLC
Produits alimentaires - Détermination de la vitamine B1 par CLHP
Ta slovenski standard je istoveten z: EN 14122:2003/AC:2005
ICS:
67.050 Splošne preskusne in General methods of tests and
analizne metode za živilske analysis for food products
proizvode
SIST EN 14122:2003/AC:2006 en,fr,de
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

---------------------- Page: 1 ----------------------

SIST EN 14122:2003/AC:2006


---------------------- Page: 2 ----------------------

SIST EN 14122:2003/AC:2006
EUROPEAN STANDARD
EN 14122:2003/AC
NORME EUROPÉENNE
December 2005
Décembre 2005
EUROPÄISCHE NORM
Dezember 2005
ICS 67.050
English version
Version Française
Deutsche Fassung
Foodstuffs - Determination of vitamin B1 by HPLC
Produits alimentaires - Détermination de la Lebensmittel - Bestimmung von Vitamin B1
vitamine B1 par CLHP mit HPLC
This corrigendum becomes effective on 7 December 2005 for incorporation in the three official
language versions of the EN.
Ce corrigendum prendra effet le 7 décembre 2005 pour incorporation dans les trois versions
linguistiques officielles de la EN.
Die Berichtigung tritt am 7.Dezember 2005 zur Einarbeitung in die drei offiziellen Sprachfassungen
der EN in Kraft.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION
EUROPÄISCHES KOMITEE FÜR NORMUNG
Management Centre: rue de Stassart, 36  B-1050 Brussels
© 2005 CEN All rights of exploitation in any form and by any means reserved worldwide for CEN national Members.
Tous droits d'exploitation sous quelque forme et de quelque manière que ce soit réservés dans le monde entier aux
membres nationaux du CEN.
Alle Rechte der Verwertung, gleich in welcher Form und in welchem Verfahren, sind weltweit den nationalen Mitgliedern
von CEN vorbehalten.
Ref. No.:EN 14122:2003/AC:2005 D/E/F

---------------------- Page: 3 ----------------------

SIST EN 14122:2003/AC:2006
EN 14122:2003/AC:2005 (E/F/D)

English version
- delete 4.2.15 and rewrite as follows:
"4.2.15 Dephosphorylation enzyme, with the ability to hydrolyse bound thiamine from food.
1)
NOTE Taka-Diastase has been used for the determination of the precision data.''

- delete footnote 1 on page 5 and rewrite as follows:
1)
Taka-Diastase Nr. T00040 is the trade name of a product supplied by Pfaltz & Bauer,
Waterbury, CT 06708, USA. This information is given for the convenience of users of this
European standard and does not constitute an endorsement by CEN of the product named.
Equivalent products may be used if they can be shown to lead to the same results.

- delete clause 6.2.2 'Enzyme treatment' and rewrite as follows:
6.2.2 Enzyme treatment
After cooling to room temperature adjust the extract to the optimal pH for the enzyme used
with sodium acetate solution (4.2.16) or (4.2.17) and add a suitable amount of
dephosphorylating enzyme (4.2.15) to the sample. Incubate the mixture at the optimal time
and temperature for the enzyme used. After cooling to room temperature transfer the solution
to a light protected volumetric flask using distilled water, or another appropriate solvent and
dilute to a defined volume (V
e).
For each enzyme used, optimal pH, incubation time and incubation temperature shall be
checked.
To ensure an optimal dephosphorylation, the enzymatic step shall be checked with analysis of
samples spiked with thiamine monophosphate chloride (4.3.3) or thiamine pyrophosphate
chloride (4.3.4), and a material similar in sample type as the test sample. This material should
be a certified reference material.
If Taka-Diastase has been used for dephosphorylation, the amount of thiamine possibly
brought in with the enzyme shall be considered in the calculation of the result.
1)
NOTE 1 For determination of the precision data given in this European standard, Taka-Diastase was
used for dephosphorylation under the following conditions. The extract was adjusted to pH = 4,0 with
sodium acetate (4.2.16) or (4.2.17) and 100 mg of Taka-Diastase per gram of sample was added. The
o o
mixture was incubated at 37 C to 46 C for 16 h to 24 h.
NOTE 2 The dephosphorylation can depend on the sample matrix and on the enzyme used. Complete
dephosphorylation can be reached within shorter time, see [8].
2

---------------------- Page: 4 ----------------------

SIST EN 14122:2003/AC:2006
EN 14122:2003/AC:2005 (E/F/D)


Version française
- supprimer 4.2.15 et le reformuler comme suit :
‘’4.2.15 enzyme déphosphorylisante, permettant d’hydrolyser la thiamine liée à partir de
l’aliment.
1)
NOTE La Takadiastase a été utilisée pour la détermination des données de fidélité’’.

- page 5, supprimer la note de bas de page 1 et la reformuler comme suit :
1)
La Takadiastase n° T00040 est l’appellation commerciale d’un produit commercialisé par
Pflatz & Bauer, Waterbury, CT 06708, USA. Cette information est donnée par souci de
commodité à l’intention des utilisateurs d
...