ISO 18862:2016
(Main)Coffee and coffee products — Determination of acrylamide — Methods using HPLC-MS/MS and GC-MS after derivatization
Coffee and coffee products — Determination of acrylamide — Methods using HPLC-MS/MS and GC-MS after derivatization
ISO 18862:2016 specifies methods for the determination of acrylamide in coffee and coffee products by extraction with water, clean-up by solid-phase extraction and determination by HPLC-MS/MS and GC-MS. It was validated in a method validation study on roasted coffee, soluble coffee, coffee substitutes and coffee products with ranges from 53 μg/kg to 612,1 μg/kg.
Café et dérivés du café — Dosage de l'acrylamide — Méthodes par CLHP-SM/SM et CG-SM après dérivation
Le présent document spécifie des méthodes de dosage de l'acrylamide dans le café et les dérivés du café par extraction à l'eau, purification par extraction en phase solide et dosage par CLHP-SM/SM et CG-SM. Il a été validé au cours d'une étude de validation de la méthode réalisée sur du café torréfié, du café soluble, des substituts de café et des dérivés du café dans des plages de concentration allant de 53 μg/kg à 612,1 μg/kg.
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DRAFT INTERNATIONAL STANDARD
ISO/DIS 18862
ISO/TC 34/SC 15 Secretariat: ABNT
Voting begins on: Voting terminates on:
2015-10-06 2016-01-06
Analysis of coffee and coffee products — Determination of
acrylamide — Methods using HPLC-MS/MS and GC-MS after
derivatisation
Analyse du café et de ses dérivés — Dosage de l’acrylamide — Méthodes utilisant CLHP-MS/MS et CG-MS
après dérivation
ICS: 67.140.20
THIS DOCUMENT IS A DRAFT CIRCULATED
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THEREFORE SUBJECT TO CHANGE AND MAY
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STANDARD UNTIL PUBLISHED AS SUCH.
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Reference number
NATIONAL REGULATIONS.
ISO/DIS 18862:2015(E)
RECIPIENTS OF THIS DRAFT ARE INVITED
TO SUBMIT, WITH THEIR COMMENTS,
NOTIFICATION OF ANY RELEVANT PATENT
RIGHTS OF WHICH THEY ARE AWARE AND TO
©
PROVIDE SUPPORTING DOCUMENTATION. ISO 2015
---------------------- Page: 1 ----------------------
ISO/DIS 18862:2015(E)
COPYRIGHT PROTECTED DOCUMENT
© ISO 2015, Published in Switzerland
All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized otherwise in any form
or by any means, electronic or mechanical, including photocopying, or posting on the internet or an intranet, without prior
written permission. Permission can be requested from either ISO at the address below or ISO’s member body in the country of
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ii © ISO 2015 – All rights reserved
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ISO/DIS 18862
Contents Page
Foreword . v
Introduction . vi
1 Scope . 1
2 Normative references . 1
3 Principle. 1
4 Reagents. 1
5 Apparatus . 3
6 Sampling. 4
7 Procedure . 4
7.1 General . 4
7.2 Preparation of the extract . 4
7.3 Clean-up of the extracts . 4
7.3.1 Carrez precipitation . 4
7.3.2 Solid phase extraction . 4
7.4 HPLC-MS/MS measurement . 5
7.4.1 High performance liquid chromatography (HPLC) . 5
7.4.2 Identification and quantification by mass spectrometry (HPLC-MS/MS) . 5
7.5 Measurement with GC-MS . 6
7.5.1 Derivatisation and sample preparation for gas chromatography . 6
7.5.2 Gas chromatography . 6
7.5.3 Ident
...
INTERNATIONAL ISO
STANDARD 18862
First edition
2016-07-15
Coffee and coffee products —
Determination of acrylamide —
Methods using HPLC-MS/MS and GC-
MS after derivatization
Café et de ses dérivés — Dosage de l’acrylamide — Méthodes utilisant
CLHP-MS/MS et CG-MS après dérivation
Reference number
ISO 18862:2016(E)
©
ISO 2016
---------------------- Page: 1 ----------------------
ISO 18862:2016(E)
COPYRIGHT PROTECTED DOCUMENT
© ISO 2016, Published in Switzerland
All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized otherwise in any form
or by any means, electronic or mechanical, including photocopying, or posting on the internet or an intranet, without prior
written permission. Permission can be requested from either ISO at the address below or ISO’s member body in the country of
the requester.
ISO copyright office
Ch. de Blandonnet 8 • CP 401
CH-1214 Vernier, Geneva, Switzerland
Tel. +41 22 749 01 11
Fax +41 22 749 09 47
copyright@iso.org
www.iso.org
ii © ISO 2016 – All rights reserved
---------------------- Page: 2 ----------------------
ISO 18862:2016(E)
Contents Page
Foreword .iv
Introduction .v
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Principle . 1
5 Reagents . 1
6 Apparatus . 3
7 Sampling . 4
8 Procedure. 4
8.1 General . 4
8.2 Preparation of the sample extract . 4
8.3 Clean-up of the extracts . 5
8.3.1 Carrez precipitation . 5
8.3.2 Solid phase extraction . 5
8.4 HPLC-MS/MS measurement . 5
8.4.1 High-performance liquid chromatography (HPLC) . 5
8.4.2 Identification and quantification by mass spectrometry (HPLC-MS/MS) . 6
8.5 Measurement with GC-MS . 6
8.5.1 Deri
...
NORME ISO
INTERNATIONALE 18862
Première édition
2016-07-15
Café et derivés du café — Dosage de
l'acrylamide — Méthodes par CLHP-
SM/SM et CG-SM après dérivation
Coffee and coffee products — Determination of acrylamide —
Methods using HPLC-MS/MS and GC-MS after derivatization
Numéro de référence
ISO 18862:2016(F)
©
ISO 2016
---------------------- Page: 1 ----------------------
ISO 18862:2016(F)
DOCUMENT PROTÉGÉ PAR COPYRIGHT
© ISO 2016
Tous droits réservés. Sauf prescription différente ou nécessité dans le contexte de sa mise en œuvre, aucune partie de cette
publication ne peut être reproduite ni utilisée sous quelque forme que ce soit et par aucun procédé, électronique ou mécanique,
y compris la photocopie, ou la diffusion sur l’internet ou sur un intranet, sans autorisation écrite préalable. Une autorisation peut
être demandée à l’ISO à l’adresse ci-après ou au comité membre de l’ISO dans le pays du demandeur.
ISO copyright office
Case postale 401 • Ch. de Blandonnet 8
CH-1214 Vernier, Genève
Tél.: +41 22 749 01 11
Fax: +41 22 749 09 47
E-mail: copyright@iso.org
Web: www.iso.org
Publié en Suisse
ii © ISO 2016 – Tous droits réservés
---------------------- Page: 2 ----------------------
ISO 18862:2016(F)
Sommaire Page
Avant-propos .iv
Introduction .v
1 Domaine d’application . 1
2 Références normatives . 1
3 Termes et définitions . 1
4 Principe . 1
5 Réactifs . 1
6 Appareillage . 3
7 Échantillonnage . 4
8 Mode opératoire. 5
8.1 Généralités . 5
8.2 Préparation de l’extrait d’échantillon. 5
8.3 Purification des extraits . 5
8.3.1 Précipitation de Carrez . . 5
8.3.2 Extraction en phase solide . 5
8.4 Analyse par CLHP-SM/SM . 6
8.4.1 Chromatographie liquide à haute performance (CLHP) . 6
8.4.2 Identification et quantification par spectrométrie de masse (CLHP-SM/SM) . 6
8.5 Analyse par CG-SM .
...
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