EN ISO 14183:2008

SLOVENSKI STANDARD
01-april-2009
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Animal feeding stuffs - Determination of monensin, narasin and salinomycin contents -
Liquid chromatographic method using post-column derivatization (ISO 14183:2005)
Futtermittel - Bestimmung der Gehalte an Monensin, Narasin und Salinomycin -
Flüssigkeitschromatographisches Verfahren mittels Nachsäulenderivatisierung (ISO
14183:2005)
Aliments des animaux - Détermination des teneurs en monensine, narasine et
salinomycine - Méthode par chromatographie liquide utilisant la dérivatisation post-
colonne (ISO 14183:2005)
Ta slovenski standard je istoveten z: EN ISO 14183:2008
ICS:
65.120 Krmila Animal feeding stuffs
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

EUROPEAN STANDARD
EN ISO 14183
NORME EUROPÉENNE
EUROPÄISCHE NORM
November 2008
ICS 65.120
English Version
Animal feeding stuffs - Determination of monensin, narasin and
salinomycin contents - Liquid chromatographic method using
post-column derivatization (ISO 14183:2005)
Aliments des animaux - Détermination des teneurs en Futtermittel - Bestimmung der Gehalte an Monensin,
monensine, narasine et salinomycine - Méthode par Narasin und Salinomycin -
chromatographie liquide utilisant la dérivatisation post- Flüssigkeitschromatographisches Verfahren mittels
colonne (ISO 14183:2005) Nachsäulenderivatisierung (ISO 14183:2005)
This European Standard was approved by CEN on 25 October 2008.
CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European
Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national
standards may be obtained on application to the CEN Management Centre or to any CEN member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by translation
under the responsibility of a CEN member into its own language and notified to the CEN Management Centre has the same status as the
official versions.
CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Cyprus, Czech Republic, Denmark, Estonia, Finland,
France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal,
Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland and United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION
EUROPÄISCHES KOMITEE FÜR NORMUNG
Management Centre: rue de Stassart, 36  B-1050 Brussels
© 2008 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 14183:2008: E
worldwide for CEN national Members.

Contents Page
Foreword.3

Foreword
The text of ISO 14183:2005 has been prepared by Technical Committee ISO/TC 34 “Agricultural food
products” of the International Organization for Standardization (ISO) and has been taken over as EN ISO
14183:2008 by Technical Committee CEN/TC 327 “Animal feeding stuffs - Methods of sampling and analysis”
the secretariat of which is held by NEN.
This European Standard shall be given the status of a national standard, either by publication of an identical
text or by endorsement, at the latest by May 2009, and conflicting national standards shall be withdrawn at the
latest by May 2009.
Attention is drawn to the possibility that some of the elements of this document may be the subject of patent
rights. CEN [and/or CENELEC] shall not be held responsible for identifying any or all such patent rights.
According to the CEN/CENELEC Internal Regulations, the national standards organizations of the following
countries are bound to implement this European Standard: Austria, Belgium, Bulgaria, Cyprus, Czech
Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia,
Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain,
Sweden, Switzerland and the United Kingdom.
Endorsement notice
The text of ISO 14183:2005 has been approved by CEN as a EN ISO 14183:2008 without any modification.

INTERNATIONAL ISO
STANDARD 14183
First edition
2005-11-01
Animal feeding stuffs — Determination of
monensin, narasin and salinomycin
contents — Liquid chromatographic
method using post-column derivatization
Aliments des animaux — Détermination des teneurs en monensine,
narasine et salinomycine — Méthode par chromatographie liquide
utilisant la dérivatisation post-colonne

Reference number
ISO 14183:2005(E)
©
ISO 2005
ISO 14183:2005(E)
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ii © ISO 2005 – All rights reserved

ISO 14183:2005(E)
Contents Page
Foreword. iv
1 Scope . 1
2 Normative references . 1
3 Principle. 1
4 Reagents. 1
5 Apparatus . 4
6 Sampling. 5
7 Preparation of test sample. 5
8 Procedure . 6
8.1 Preparation of quality control sample . 6
8.2 Extraction . 6
8.3 HPLC analysis . 7
8.4 Determination. 8
9 HPLC confirmation . 9
9.1 General. 9
9.2 Post-column derivatization with DMAB. 9
9.3 Hexane extraction. 10
10 Calculation of results . 10
10.1 General. 10
10.2 Monensin . 10
10.3 Salinomycin. 11
10.4 Narasin. 12
10.5 Interpretation of confirmation data. 13
11 Precision. 14
11.1 Interlaboratory test . 14
11.2 Repeatability. 14
11.3 Reproducibility. 14
11.4 Limit of quantitation . 15
12 Test report . 15
Annex A (informative) Results of interlaboratory test. 16
Bibliography . 21

ISO 14183:2005(E)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards bodies
(ISO member bodies). The work of preparing International Standards is normally carried out through ISO
technical committees. Each member body interested in a subject for which a technical committee has been
established has the right to be represented on that committee. International organizations, governmental and
non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely with the
International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization.
International Standards are drafted in accordance with the rules given in the ISO/IEC Directives, Part 2.
The main task of technical committees is to prepare International Standards. Draft International Standards
adopted by the technical committees are circulated to the member bodies for voting. Publication as an
International Standard requires approval by at least 75 % of the member bodies casting a vote.
Attention is drawn to the possibility that some of the elements of this document may be the subject of patent
rights. ISO shall not be held responsible for identifying any or all such patent rights.
ISO 14183 was prepared by Technical Committee ISO/TC 34, Food products, Subcommittee SC 10, Animal
feeding stuffs.
iv © ISO 2005 – All rights reserved

INTERNATIONAL STANDARD ISO 14183:2005(E)

Animal feeding stuffs — Determination of monensin, narasin
and salinomycin contents — Liquid chromatographic method
using post-column derivatization
1 Scope
This International Standard specifies a high-performance liquid chromatographic (HPLC) method for the
determination of the monensin, narasin and salinomycin contents of animal feeding stuffs, supplements (dry
and liquid) and mineral premixtures. The method is not applicable to drug premixes (pharmaceutical products).
Lasalocid and semduramicin cannot be determined by this method.
The limit of quantitation is approximately 1 mg/kg, 2 mg/kg and 2 mg/kg for monensin, salinomycin and
narasin, respectively. A lower limit of quantitation can be achievable but this is to be validated by the user.
2 Normative references
The following referenced documents are indispensable for the application of this document. For dated
references, only the edition cited applies. For undated references, the latest edition of the referenced
document (including any amendments) applies.
ISO 6498:1998, Animal feeding stuffs — Preparation of test samples
3 Principle
The ionophores monensin, narasin and salinomycin are extracted using methanol/water (90 + 10) with
mechanical shaking for 1 h, then the extracts are fi
...