EN ISO 6647-1:2020

SLOVENSKI STANDARD
01-oktober-2020
Riž - Določevanje amiloze - 1. del: Spektrofotometrijska metoda s postopkom
razmaščevanja z metanolom in s kalibracijskima raztopinama amiloza krompirja
ter voskast amilopektin riža (ISO 6647-1:2020)
Rice - Determination of amylose content - Part 1: Spectrophotometric method with a
defatting procedure by methanol and with calibration solutions of potato amylose and
waxy rice amylopectin (ISO 6647-1:2020)
Reis - Bestimmung des Amylosegehalts - Teil 1: Referenzmethode:
Spektrophotometrische Methode mit Entfettungsverfahren durch Methanol und mit
Kalibrierlösungen von Kartoffelamylose und wachsartigem Reisamylopektin (ISO 6647-
1:2020)
Riz - Détermination de la teneur en amylose - Partie 1: Méthode spectrophotométrique
avec un mode opératoire de dégraissage au méthanol et des solutions d'étalonnage
d'amylose de pomme de terre et d'amylopectine de riz gluant (ISO 6647-1:2020)
Ta slovenski standard je istoveten z: EN ISO 6647-1:2020
ICS:
67.060 Žita, stročnice in proizvodi iz Cereals, pulses and derived
njih products
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

EN ISO 6647-1
EUROPEAN STANDARD
NORME EUROPÉENNE
August 2020
EUROPÄISCHE NORM
ICS 67.060 Supersedes EN ISO 6647-1:2015
English Version
Rice - Determination of amylose content - Part 1:
Spectrophotometric method with a defatting procedure by
methanol and with calibration solutions of potato amylose
and waxy rice amylopectin (ISO 6647-1:2020)
Riz - Détermination de la teneur en amylose - Partie 1: Reis - Bestimmung des Amylosegehalts - Teil 1:
Méthode spectrophotométrique avec un mode Referenzmethode: Spektrophotometrische Methode
opératoire de dégraissage au méthanol et des solutions mit Entfettungsverfahren durch Methanol und mit
d'étalonnage d'amylose de pomme de terre et Kalibrierlösungen von Kartoffelamylose und
d'amylopectine de riz gluant (ISO 6647-1:2020) wachsartigem Reisamylopektin (ISO 6647-1:2020)
This European Standard was approved by CEN on 18 July 2020.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this
European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by
translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management
Centre has the same status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and
United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2020 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 6647-1:2020 E
worldwide for CEN national Members.

Contents Page
European foreword . 3

European foreword
This document (EN ISO 6647-1:2020) has been prepared by Technical Committee ISO/TC 34 "Food
products" in collaboration with Technical Committee CEN/TC 338 “Cereal and cereal products” the
secretariat of which is held by AFNOR.
This European Standard shall be given the status of a national standard, either by publication of an
identical text or by endorsement, at the latest by February 2021, and conflicting national standards
shall be withdrawn at the latest by February 2021.
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. CEN shall not be held responsible for identifying any or all such patent rights.
This document supersedes EN ISO 6647-1:2015.
According to the CEN-CENELEC Internal Regulations, the national standards organizations of the
following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria,
Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland,
Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Republic of
North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and the
United Kingdom.
Endorsement notice
The text of ISO 6647-1:2020 has been approved by CEN as EN ISO 6647-1:2020 without any
modification.
INTERNATIONAL ISO
STANDARD 6647-1
Third edition
2020-07
Rice — Determination of amylose
content —
Part 1:
Spectrophotometric method with a
defatting procedure by methanol and
with calibration solutions of potato
amylose and waxy rice amylopectin
Riz — Détermination de la teneur en amylose —
Partie 1: Méthode spectrophotométrique avec un mode opératoire de
dégraissage au méthanol et des solutions d'étalonnage d'amylose de
pomme de terre et d'amylopectine de riz gluant
Reference number
ISO 6647-1:2020(E)
©
ISO 2020
ISO 6647-1:2020(E)
© ISO 2020
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Published in Switzerland
ii © ISO 2020 – All rights reserved

ISO 6647-1:2020(E)
Contents Page
Foreword .iv
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Principle . 2
5 Reagents . 2
6 Apparatus . 3
7 Sampling . 4
8 Procedure. 4
8.1 Preparation of test sample . 4
8.2 Test portion and preparation of the test solution . 4
8.3 Preparation of the blank solution . 4
8.4 Preparation of the calibration graph . 4
8.4.1 Preparation of the set of calibration solutions . 4
8.4.2 Colour development and spectrophotometric measurements . 5
8.4.3 Plotting the calibration graph . 5
8.5 Determination . 5
9 Expression of results . 5
10 Precision . 5
10.1 Interlaboratory test. 5
10.2 Repeatability . 6
10.3 Reproducibility . 6
11 Test report . 6
Annex A (informative) Determination of the quality of the potato amylose stock suspension .7
Annex B (informative) Results of an interlaboratory test . 9
Annex C (informative) Example of a flow injection analyser (FIA) for the determination of
amylose .10
Bibliography .11
ISO 6647-1:2020(E)
Foreword
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electrotechnical standardization.
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different types of ISO documents should be noted. This document was drafted in accordance with the
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patent rights. ISO shall not be held responsible for identifying any or all such patent rights. Details of
any patent rights identified during the development of the document will be in the Introduction and/or
on the ISO list of patent declarations received (see www .iso .org/ patents).
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iso/ foreword .html.
This document was prepared by Technical Committee ISO/TC 34, Food products, Subcommittee SC 4,
Cereals and pulses, in collaboration with the European Committee for Standardization (CEN) Technical
Committee CEN/TC 338, Cereal and cereal products, in accordance with the Agreement on technical
cooperation between ISO and CEN (Vienna Agreement).
This third ed
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