EN 14164:2014

2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.Lebensmittel - Bestimmung von Vitamin B6 mit Hochleistungs-FlüssigchromatographieProduits alimentaires - Dosage de la vitamine B6 par chromatographie liquide haute performanceFoodstuffs - Determination of vitamin B6 by high performance chromatography67.050Splošne preskusne in analizne metode za živilske proizvodeGeneral methods of tests and analysis for food productsICS:Ta slovenski standard je istoveten z:EN 14164:2014SIST EN 14164:2014en,fr,de01-september-2014SIST EN 14164:2014SLOVENSKI
STANDARDSIST EN 14164:20081DGRPHãþD

EUROPEAN STANDARD NORME EUROPÉENNE EUROPÄISCHE NORM
EN 14164
June 2014 ICS 67.050 Supersedes EN 14164:2008English Version
Foodstuffs - Determination of vitamin B6 by high performance chromatography
Produits alimentaires - Détermination de la teneur en vitamine B6 par chromatographie liquide haute performance Lebensmittel - Bestimmung von Vitamin B6 mit Hochleistungs-Flüssigchromatographie This European Standard was approved by CEN on 17 April 2014.
CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management Centre has the same status as the official versions.
CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION EUROPÄISCHES KOMITEE FÜR NORMUNG
CEN-CENELEC Management Centre:
Avenue Marnix 17,
B-1000 Brussels © 2014 CEN All rights of exploitation in any form and by any means reserved worldwide for CEN national Members. Ref. No. EN 14164:2014 ESIST EN 14164:2014

Example of a chromatogram . 11 Annex B (informative)
Precision data . 12 Annex C (informative)
Sample treatment option without acid hydrolysis . 14 Annex D (informative)
Examples for molar absorption coefficients . 15 Bibliography . 16
1) Katal (symbol ''kat'') is a derived SI unit of enzyme activity. One katal is that catalytic activity which will raise the rate of reaction by one mol/s in a specified assay system. 2) This information is given for the convenience of users of this European standard and does not constitute an endorsement by CEN of the supplier. Equivalent products may be used if they can be shown to lead to the same results. SIST EN 14164:2014

4.23 Pyridoxine hydrochloride (vitamin B6 standard substance), w(C8H11NO3 HCI) ≥ 99 %. 4.24 Pyridoxine hydrochloride stock solution, mass concentration
≈ 0f5 mg/ml. Dissolve an accurately weighed amount of pyridoxine hydrochloride (4.23), e.g. 50 mg, in a defined volume, e.g. 100 ml, of water. The stock solution is stable for 4 weeks if stored at 4 °C in the dark. For the concentration test, dilute 0,5 ml of pyridoxine hydrochloride stock solution (4.24) to 20 ml with 0,1 mol/l HCI (4.20) and measure the absorbance at 290 nm in a 1 cm cell using a UV-spectrometer (5.1) against 0
...