EN 14152:2003

2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.Lebensmittel - Bestimmung von Vitamin B2 mit HPLCProduits alimentaires - Détermination de la teneur en vitamine B2 par CLHPFoodstuffs - Determination of vitamin B2 by HPLC67.050Splošne preskusne in analizne metode za živilske proizvodeGeneral methods of tests and analysis for food productsICS:Ta slovenski standard je istoveten z:EN 14152:2003SIST EN 14152:2003en01-november-2003SIST EN 14152:2003SLOVENSKI
STANDARD
EUROPEAN STANDARDNORME EUROPÉENNEEUROPÄISCHE NORMEN 14152July 2003ICS 67.050English versionFoodstuffs - Determination of vitamin B2 by HPLCProduits alimentaires - Dosage de la vitamine B2 par CLHPLebensmittel - Bestimmung von Vitamin B2 mit HPLCThis European Standard was approved by CEN on 2 May 2003.CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this EuropeanStandard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such nationalstandards may be obtained on application to the Management Centre or to any CEN member.This European Standard exists in three official versions (English, French, German). A version in any other language made by translationunder the responsibility of a CEN member into its own language and notified to the Management Centre has the same status as the officialversions.CEN members are the national standards bodies of Austria, Belgium, Czech Republic, Denmark, Finland, France, Germany, Greece,Hungary, Iceland, Ireland, Italy, Luxembourg, Malta, Netherlands, Norway, Portugal, Slovakia, Spain, Sweden, Switzerland and UnitedKingdom.EUROPEAN COMMITTEE FOR STANDARDIZATIONCOMITÉ EUROPÉEN DE NORMALISATIONEUROPÄISCHES KOMITEE FÜR NORMUNGManagement Centre: rue de Stassart, 36
B-1050 Brussels© 2003 CENAll rights of exploitation in any form and by any means reservedworldwide for CEN national Members.Ref. No. EN 14152:2003 ESIST EN 14152:2003

Figure.11Annex B (informative)
Precision data.12Annex C (informative)
Alternative HPLC-Systems.13Bibliography.14SIST EN 14152:2003

mass fraction, w(CH3OH)
% HPLC grade4.2.2 Sodium acetate trihydrate, w(CH3COONa · 3H2O) = 99 %4.2.3 Sodium acetate solution, substance concentration c(CH3COONa · 3H2O) = 0,1 mol/l4.2.4 Sodium acetate solution, c(CH3COONa · 3H2O) = 2,5 mol/l4.2.5 Glacial acetic acid, w(CH3COOH) = 99,8 %4.2.6 Acetic acid solution, c(CH3COOH) = 0,02 mol/l4.2.7 Hydrochloric acid, w(HCl) = 36 %4.2.8 Hydrochloric acid, c(HCl) = 0,1 mol/l4.2.9 Hydrochloric acid, c(HCl) = 0,01 mol/l4.2.10 Sulfuric acid, c(H2SO4) = 0,05 mol/l4.2.11 Sodium hydroxide, w(NaOH) = 99 %4.2.12 Sodium hydroxide solution, c(NaOH) = 0,5 mol/l4.2.13 Phosphorous pentoxide, w(P2O5) = 98 %4.2.14 Dephosphorylating enzyme,
with the ability to hydrolyse bound riboflavin from food1)4.2.15 HPLC Mobile phaseExamples of appropriate mixtures of e. g. 10 % to 50 % methanol (4.2.1) in water or using phosphate oracetate buffer are given in annex A1 and C; possibility of using ion-pairing agents is also given.
1)e.g. Taka-Diastase Nr. T00040, Pfalz & Bauer, Waterbury, CT 06708, USA was used for the collaborative study. Thisinformation is given for the convenience of users of this standard and does not constitute endorsement by CEN of theproduct named. Equivalent products may be used if they can be shown to lead to the same results [7, 10, 11].SIST EN 14152:2003
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