ASTM D2574-06

Designation: D2574 − 06
Standard Test Method for
Resistance of Emulsion Paints in the Container to Attack by
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Microorganisms
This standard is issued under the fixed designation D2574; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision. A number in parentheses indicates the year of last reapproval. A
superscript epsilon (´) indicates an editorial change since the last revision or reapproval.
1. Scope* evaluatetheperformanceinemulsionpaintsofcompetitiveand
developmental preservatives.
1.1 This test method covers the determination of the relative
resistance of emulsion paints to attack in the container by
4.2 This test method should be used preferably by persons
microorganisms.
who have had basic microbiological training.
1.2 The values stated in SI units are to be regarded as the
NOTE 1—The reliability of the results obtained from this test method is
standard. The values given in parentheses are for information
extremely dependent on the techniques employed. Improper techniques
can result in a sterile sample appearing to be contaminated, and even
only.
worse, a contaminated sample appearing to be sterile (see also Note 2). It
1.3 This standard does not purport to address all of the
is recommended that you consult with your biocide supplier, raw material
safety concerns, if any, associated with its use. It is the
supplier, or an independent testing laboratory to confirm questionable
responsibility of the user of this standard to establish appro- results. Formulation and raw materials’ quality may also vary and thereby
affect the test results.
priate safety and health practices and determine the applica-
bility of regulatory limitations prior to use.
5. Apparatus and Materials
2. Referenced Documents
5.1 Balance, capable of weighing to 0.10 g.
2
2.1 ASTM Standards:
5.2 Incubator, or other device capable of maintaining a
D5588 Test Method for Determination of the Microbial
constant temperature between 28 and 32°C.
Condition of Paint, Paint Raw Materials, and Plant Areas
5.3 Refrigerator, maintained at 10 to 13°C.
3. Summary of Test Method
5.4 Screwcap Borosilicate Test Tubes, 125 by 15-mm.
3.1 Thistestmethodisdesignedtochallengesamplesofone
5.5 Borosilicate Flasks, 1-L.
or more paints containing various levels of one or more
5.6 Screwcap Bottles, 150-mL.
biocides with a known amount of bacteria and rate the ability
of the test paint(s) to control the “contamination.”
5.7 Autoclave, capable of producing 103 kPa (15 psi) of
steam pressure at 121°C and maintaining it for a minimum of
4. Significance and Use
15 min. An autoclave is not necessary if prepared agar slants
4.1 Spoilage of paint in the container can result in putrefac- are used.
tion,loweredpH,gasformation,anddecreaseinviscosity.This
5.8 Pipettes or an Automatic Pipettor , sterile, 1-mL, with
test method provides a standard procedure for the evaluation of
sterile disposable pipette tips for 1 mL.
the resistance of emulsion paints to microbial deterioration.
5.9 Petri Dishes, sterile.
The results should enable: (1) the paint manufacturer to select
aneffectivepreservativeand(2)thesupplierofpreservativesto
5.10 Dehydrated Tryptic Soy Agar (TSA), medium, or pre-
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prepared slants, plates, and broth tubes.
5.11 Swabs, sterile cotton.
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This test method is under the jurisdiction of ASTM Committee D01 on Paint
and Related Coatings, Materials, andApplications and is the direct responsibility of
Subcommittee D01.28 on Biodeterioration.
Current edition approved June 1, 2006. Published June 2006. Originally
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approved in 1967. Last previous edition approved in 2000 as D2574 – 00. DOI: Available from microbiological supply companies. Media with TTC indicator
10.1520/D2574-06. dye may be used. In general, the TTC helps visualize contamination, but it has been
2
For referenced ASTM standards, visit the ASTM website, www.astm.org, or reported on occasion to inhibit the growth of some bacteria. Interferences from
contact ASTM Customer Service at service@astm.org. For Annual Book of ASTM pigments in materials being tested may make the color change difficult to see. If
Standards volume information, refer to the standard’s Document Summary page on self-prepared plates are used with the TTC indicator, 0.01 % TTC indicator should
the ASTM website. be used and it must be added after autoclaving.
*A Summary of Changes section appears at the end of this standard
Copyright © ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959. United States
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D2574 − 06
5.12 Laminar Flow Hood, Sterile Room, or at Least a off with a sterile inoculating loop. This material is inoculated
Laboratory Testing Area, relatively clean, free of blowing dust into a tube ofTSB each and incubated in a 30 6 2°C incubator
and dirt, etc., which can be used for streaking plates. overnight.
6.3.2 The overnight cultures are used t
...