ASTM E982-94(2021)
(Specification)Standard Specification for Laboratory Glass Test Tubes
Standard Specification for Laboratory Glass Test Tubes
ABSTRACT
This specification covers glass test tubes suitable for laboratory use. The test tubes shall be of the following classification according to size: Type I, Type II, Type III, Type IV (Class A, Class B, and Class C), Type V, Type VI, and Type VII. Test tubes shall be made of the prescribed borosilicate glass (Type I glass) or of soda-lime glass (Type II glass) and shall be tubular, with one end having a rounded closure. The following are specified: (1) material requirements for screw caps, liners, and annealing and (2) design requirements for the test tube capacity, dimensions (OD, length, and wall thickness), and area for marking. Other dimensional requirements such as subdivision, stopper, and GPI thread finish are also specified.
SCOPE
1.1 This specification covers glass test tubes suitable for laboratory use.
1.2 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.
General Information
- Status
- Published
- Publication Date
- 31-Dec-2020
- Technical Committee
- E41 - Laboratory Apparatus
- Drafting Committee
- E41.01 - Laboratory Ware and Supplies
Relations
- Effective Date
- 01-Feb-2024
- Effective Date
- 01-Oct-2023
- Effective Date
- 01-Jul-2019
- Refers
ASTM E675-02(2019) - Standard Specification for Interchangeable Taper-Ground Stopcocks And Stoppers - Effective Date
- 01-Jan-2019
- Effective Date
- 01-Jan-2018
- Effective Date
- 01-Nov-2015
- Effective Date
- 01-Nov-2013
- Refers
ASTM E675-02(2012) - Standard Specification for Interchangeable Taper-Ground Stopcocks And Stoppers - Effective Date
- 01-Nov-2012
- Effective Date
- 01-Dec-2011
- Effective Date
- 01-Dec-2011
- Effective Date
- 01-Dec-2011
- Effective Date
- 01-Sep-2010
- Effective Date
- 15-May-2010
- Effective Date
- 01-Apr-2010
- Effective Date
- 01-Nov-2008
Overview
ASTM E982-94(2021) - Standard Specification for Laboratory Glass Test Tubes defines the essential requirements for glass test tubes intended for laboratory uses. Developed and maintained by ASTM International, this specification ensures that glass test tubes meet rigorous criteria for material quality, design, dimensions, and performance, promoting safety and consistency across laboratories worldwide. The standard classifies test tubes by type and size and specifies requirements for materials such as borosilicate glass and soda-lime glass, as well as details for caps and liners, annealing, and marking areas.
Key Topics
Classification of Test Tubes
The standard categorizes test tubes into multiple types (Type I through VIII), based on size, features, and intended application, covering beaded rim, graduated, stopper, culture, screw-cap, sidearm, and heavy-wall varieties.Material Requirements
- Glass: Test tubes must be made of prescribed borosilicate glass (Type I) or soda-lime glass (Type II), ensuring chemical durability and resistance to thermal shock.
- Screw Caps and Liners: Specifies the use of phenol-formaldehyde or polypropylene resins for caps and white rubber or PTFE for liners, ensuring compatibility for autoclaving and appropriate sealing for various laboratory processes.
- Annealing: Tubes must meet thermal stress limits for laboratory glassware.
Design and Dimensional Requirements
- Dimensions: Test tubes must conform to prescribed outer diameter, length, wall thickness, and capacity ranges for each size and type.
- Shape: All tubes should be tubular with a rounded closure at one end; open ends may be beaded, rimless, fitted for screw caps, or have a sidearm.
- Marking Area: Laboratories benefit from durable, markable areas for labeling.
Other Requirements
The standard addresses stopper compatibility, thread finish (GPI), and subdivision markings for graduated tubes, supporting a wide range of experimental applications.
Applications
This specification is fundamental for users and manufacturers who require consistent, high-quality laboratory glassware. Key applications include:
- Clinical and Research Laboratories: Ensures reliability and safety for sample collection, chemical reactions, heating, and storage.
- Manufacturers of Laboratory Supplies: Enables the production and certification of laboratory test tubes that meet internationally recognized standards.
- Procurement and Quality Assurance: Assists purchasing departments in selecting appropriate products and performing quality checks according to ASTM guidelines.
- Calibration and Testing: Provides a reference for capacity and dimensional accuracy required for analytical and experimental protocols.
Following ASTM E982-94(2021) helps laboratories maintain compliance, improve compatibility across equipment, and minimize risks associated with substandard glassware.
Related Standards
To ensure a comprehensive approach to laboratory glassware quality, users should also refer to the following ASTM and industry standards:
- ASTM E438 - Specification for Glasses in Laboratory Apparatus
- ASTM E1157 - Specification for Sampling and Testing of Reusable Laboratory Glassware
- ASTM E920 - Specification for Commercially Packaged Laboratory Apparatus
- ASTM E921 - Specification for Export Packaged Laboratory Apparatus
- ASTM E671 - Specification for Maximum Permissible Thermal Residual Stress in Annealed Glass Laboratory Apparatus
- Glass Packaging Institute (GPI) Standards - For thread finish and packaging
- ASTM E542 - Practice for Calibration of Laboratory Volumetric Apparatus
By adhering to ASTM E982 and related standards, laboratories, academic institutions, and manufacturers support the highest levels of safety, precision, and quality in laboratory operations and glassware supply.
Keywords: laboratory glass test tubes, ASTM E982, laboratory glassware standards, borosilicate glass, soda-lime glass, screw caps, test tube specifications, laboratory equipment.
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Frequently Asked Questions
ASTM E982-94(2021) is a technical specification published by ASTM International. Its full title is "Standard Specification for Laboratory Glass Test Tubes". This standard covers: ABSTRACT This specification covers glass test tubes suitable for laboratory use. The test tubes shall be of the following classification according to size: Type I, Type II, Type III, Type IV (Class A, Class B, and Class C), Type V, Type VI, and Type VII. Test tubes shall be made of the prescribed borosilicate glass (Type I glass) or of soda-lime glass (Type II glass) and shall be tubular, with one end having a rounded closure. The following are specified: (1) material requirements for screw caps, liners, and annealing and (2) design requirements for the test tube capacity, dimensions (OD, length, and wall thickness), and area for marking. Other dimensional requirements such as subdivision, stopper, and GPI thread finish are also specified. SCOPE 1.1 This specification covers glass test tubes suitable for laboratory use. 1.2 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.
ABSTRACT This specification covers glass test tubes suitable for laboratory use. The test tubes shall be of the following classification according to size: Type I, Type II, Type III, Type IV (Class A, Class B, and Class C), Type V, Type VI, and Type VII. Test tubes shall be made of the prescribed borosilicate glass (Type I glass) or of soda-lime glass (Type II glass) and shall be tubular, with one end having a rounded closure. The following are specified: (1) material requirements for screw caps, liners, and annealing and (2) design requirements for the test tube capacity, dimensions (OD, length, and wall thickness), and area for marking. Other dimensional requirements such as subdivision, stopper, and GPI thread finish are also specified. SCOPE 1.1 This specification covers glass test tubes suitable for laboratory use. 1.2 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.
ASTM E982-94(2021) is classified under the following ICS (International Classification for Standards) categories: 71.040.20 - Laboratory ware and related apparatus. The ICS classification helps identify the subject area and facilitates finding related standards.
ASTM E982-94(2021) has the following relationships with other standards: It is inter standard links to ASTM E438-92(2024), ASTM C162-23, ASTM E920-97(2019), ASTM E675-02(2019), ASTM E438-92(2018), ASTM C162-05(2015), ASTM E920-97(2013), ASTM E675-02(2012), ASTM E1157-87(2011), ASTM E438-92(2011), ASTM E1157-87(2018)e1, ASTM E1133-86(2010), ASTM E921-97(2010), ASTM E671-98(2010), ASTM E920-97(2008). Understanding these relationships helps ensure you are using the most current and applicable version of the standard.
ASTM E982-94(2021) is available in PDF format for immediate download after purchase. The document can be added to your cart and obtained through the secure checkout process. Digital delivery ensures instant access to the complete standard document.
Standards Content (Sample)
This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the
Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.
Designation: E982 −94 (Reapproved 2021)
Standard Specification for
Laboratory Glass Test Tubes
This standard is issued under the fixed designation E982; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision. A number in parentheses indicates the year of last reapproval. A
superscript epsilon (´) indicates an editorial change since the last revision or reapproval.
1. Scope E1157 Specification for Sampling and Testing of Reusable
Laboratory Glassware
1.1 This specification covers glass test tubes suitable for
2.2 Glass Packaging Institute Standard:
laboratory use.
GPI Tall Continuous Thread Concealed Bead Finish, Glass
1.2 This international standard was developed in accor-
Finish Drawing Nos. 410, 415, and 430
dance with internationally recognized principles on standard-
ization established in the Decision on Principles for the
3. Classification
Development of International Standards, Guides and Recom-
3.1 Test tubes shall be of the following sizes:
mendations issued by the World Trade Organization Technical
Size Nominal OD × Length, mm
Barriers to Trade (TBT) Committee.
1 6×50
2 10×70
2. Referenced Documents
3 10×75
4 12×75
2.1 ASTM Standards:
5 12 × 100
C162 Terminology of Glass and Glass Products
6 13 × 100
D700 Specification for Phenolic Molding Compounds
7 14 × 100
8 14 × 150
(Withdrawn 1990)
9 15 × 125
D2146 Specification for Propylene Plastic Molding and
10 16 × 100
Extrusion Materials (Withdrawn 1986)
11 16 × 125
12 16 × 150
E438 Specification for Glasses in Laboratory Apparatus
13 18 × 150
E542 Practice for Calibration of Laboratory Volumetric
14 19 × 150
Apparatus
15 20 × 125
16 20 × 150
E671 Specification for Maximum Permissible Thermal Re-
17 21 × 150
sidual Stress in Annealed Glass Laboratory Apparatus
18 22 × 175
E675 Specification for Interchangeable Taper-Ground Stop- 19 25 × 150
20 25 × 200
cocks And Stoppers
21 25 × 250
E920 Specification for Commercially Packaged Laboratory
22 38 × 200
Apparatus
3.1.1 Type I—General-Purpose, Beaded Rim:
E921 Specification for Export Packaged Laboratory Appa-
Size Nominal OD × Length, mm
ratus
3 10×75
E1133 Practice for Performance Testing of Packaged Labo-
4 12×75
ratory Apparatus for United States Government Procure-
6 13 × 100
9 15 × 125
ments
11 16 × 125
12 16 × 150
13 18 × 150
This specification is under the jurisdiction of ASTM Committee E41 on
14 19 × 150
Laboratory Apparatus and is the direct responsibility of Subcommittee E41.01 on
16 20 × 150
Laboratory Ware and Supplies.
19 25 × 150
Current edition approved Jan. 1, 2021. Published February 2021. Originally
20 25 × 200
approvedin1984.Lastpreviouseditionapprovedin2015asE982 – 94(2015).DOI:
10.1520/E0982-94R21.
3.1.2 Type II—Beaded Rim, Graduated:
For referenced ASTM standards, visit the ASTM website, www.astm.org, or
Size Nominal OD × Length, mm
contact ASTM Customer Service at service@astm.org. For Annual Book of ASTM
Standards volume information, refer to the standard’s Document Summary page on
the ASTM website.
3 4
The last approved version of this historical standard is referenced on Available from Glass Packaging Institute, 6845 Elm Street, Suite 209, McLean,
www.astm.org. VA 22101.
Copyright © ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959. United States
E982 − 94 (2021)
accordance with the International System of Units (SI).
5 12 × 100
8 14 × 150
11 16 × 125
4. Material
12 16 × 150
16 20 × 150
4.1 Test Tubes—Test tubes shall be made of borosilicate
17 21 × 150
glass conforming to the requirements of Type I or of soda-lime
20 25 × 200
glass conforming to the requirements of Type II glass of
3.1.3 Type III—Standard Taper, Glass-Stoppered:
Specification E438.
Size Nominal OD × Length, mm
4.2 Screw Caps—ClassAand B screw caps shall be molded
6 13 × 100
12 16 × 150
of phenol-formaldehyde type resin, which is free of cellulose
14 19 × 150
acetate and urea, in accordance with Specification D700. Class
18 22 × 175
C screw cap shall be molded of polypropylene resin in
20 25 × 200
accordance with Specification D2146. Screw caps shall not
3.1.4 Type IV—Culture Tubes, Rimless:
deform, crack, turn color, or become tacky; and liners shall not
Size Nominal OD × Length, mm
detach or become tacky when autoclaved the first time.
1 6×50
3 10×75
4.3 Liners—Liners for Class A screw caps shall be fabri-
4 12×75
cated of resilient white rubber. Class B caps shall have a
6 13 × 100
polytetrafluoroethylene liner interface firmly bonded to the
9 15 × 125
10 16 × 100
glued-in white rubber liner as described for Class A caps.
11 16 × 125
12 16 × 150
4.4 Annealing—Maximum thermal residual stress shall be
13 18 × 150
in accordance with Specification E671.
14 19 × 150
16 20 × 150
18 22 × 125
5. Design
19 25 × 150
20 25 × 200
5.1 Shape—Test tubes covered by this specification shall be
21 25 × 250
tubular, with one end having a rounded closure.
3.1.5 Type V—Culture Tubes, Filtered for Wave Lengths in
5.2 Capacity—Capacities on ungraduated tubes are only
the 3000 to 5000-Å Range:
approximate, but any graduation onType II tubes shall indicate
Size Nominal OD × Length, mm
the contained capacity within 65 % of nominal tube capacity.
13 18 × 150
19 25 × 150
5.3 TypesIandII—TheopenendofTypesIandIItesttubes
3.1.6 Type VI—Culture Tubes, Screw Caps:
shall have a beaded rim; dimensions shall be as specified in
Table 1.
Size Nominal OD × Length, mm
6 13 × 100
5.4 Type III—The open end of Type III test tub
...




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